Výsledky vyhledávání

Rapid engineering of bacterial artificial chromosomes using oligonucleotides

Autor: S, Swaminathan, H M, Ellis, L S, Waters, D, Yu, E C, Lee, D L, Court, S K, Sharan

Publikováno v: Genesis (New York, N.Y. : 2000). 29(1)

A rapid method obviating the use of selectable markers to genetically manipulate large DNA inserts cloned into bacterial artificial chromosomes is described. Mutations such as single-base changes, deletions, and insertions can be recombined into a BA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e3e7e5de9e52060452ea8a3fa4f661a6
https://pubmed.ncbi.nlm.nih.gov/11135458

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Purification, characterization and crystallization of ERA, an essential GTPase from Escherichia coli

Autor: X, Chen, S M, Chen, B S, Powell, D L, Court, X, Ji

Publikováno v: FEBS letters. 445(2-3)

ERA is an essential GTPase widely conserved in bacteria. Homologues of ERA are also present in higher eukaryotic cells. ERA is involved in bacterial cell cycle control at a point preceding cell division. In order to aid the functional investigation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4791dfa7022c28033e6f1c9d6ebde13c
https://pubmed.ncbi.nlm.nih.gov/10094501

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A new system to place single copies of genes, sites and lacZ fusions on the Escherichia coli chromosome

Autor: D, Yu, D L, Court

Publikováno v: Gene. 223(1-2)

To place a single-copy lacZ fusion on the E. coli chromosome, a method was developed based on in vivo homologous DNA recombination through P1 transduction. The fusions, initially constructed on plasmids, are crossed to lambdalacZ fusion vectors which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::eec2b7c76b6af79b0a5321080aea88fc
https://pubmed.ncbi.nlm.nih.gov/9858691

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Sequential assignments and secondary structure of the RNA-binding transcriptional regulator NusB

Autor: A S, Altieri, M J, Mazzulla, H, Zhou, N, Costantino, D L, Court, R A, Byrd

Publikováno v: FEBS letters. 415(2)

The NusB protein is involved in transcriptional regulation in bacteriophage lambda. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::541f954174f313d7a1a026aebd7475f5
https://pubmed.ncbi.nlm.nih.gov/9351000

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Transcription Termination Signals in the Nin Region of Bacteriophage Lambda: Identification of Rho-Dependent Termination Regions

Autor: D. I. Friedman, Sheau Wei C. Cheng, D. L. Court

The approximately 3-kb nin region of bacteriophage lambda, located between genes P and Q contains transcription termination signals as well as 10 open reading frames. Deletions in the nin region frees phage growth from dependence on the lambda-encode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2edfa7444f6db809e618e73b59a872c9
https://europepmc.org/articles/PMC1206672/

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Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant

Autor: B S, Powell, D L, Court, T, Inada, Y, Nakamura, V, Michotey, X, Cui, A, Reizer, M H, Saier, J, Reizer

Publikováno v: The Journal of biological chemistry. 270(9)

Two rpoN-linked delta Tn10-kan insertions suppress the conditionally lethal erats allele. One truncates rpoN while the second disrupts another gene (ptsN) in the rpoN operon and does not affect classical nitrogen regulation. Neither alter expression

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6c05b17bb69a8737d47289bc17d263f8
https://pubmed.ncbi.nlm.nih.gov/7876255

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Bacteriophage lambda N-dependent transcription antitermination. Competition for an RNA site may regulate antitermination

Autor: T A, Patterson, Z, Zhang, T, Baker, L L, Johnson, D I, Friedman, D L, Court

Publikováno v: Journal of molecular biology. 236(1)

Bacteriophage lambda controls the expression of its early genes in a temporal manner by a series of transcription termination and antitermination events. This antitermination requires the lambda N protein as well as host proteins called Nus, and cis-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::033eb80301d24211bc5a0e4a100f6058
https://pubmed.ncbi.nlm.nih.gov/8107107

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