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Intraluminal proteolytic activation plays an important role in replication of type 1 reovirus in the intestines of neonatal mice

Autor: B N Fields, Rina Dambrauskas, D K Bodkin, D M Bass, Jerry S. Trier, Jacqueline L. Wolf

Publikováno v: Journal of virology. 64(4)

Oral inoculation of suckling mice with reovirus serotype 1 (strain Lang) results in the conversion of intact virions to intermediate subviral particles (ISVPs) in the intestinal lumen. Digestion of virus in vitro with chymotrypsin or trypsin reveals

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dd8b9a4c2a20e94550d71fe5e3b24a5
https://pubmed.ncbi.nlm.nih.gov/2157065

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Autor: D. L. Knudson, D. K. Bodkin

Publikováno v: Journal of General Virology. 67:683-691

Cognate genes of members of the Palyam serogroup of orbiviruses have been identified previously, and their relatedness to the prototype virus was determined by blot hybridization of the genome segments of members of the serogroup using Palyam genomic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::232f9851288980c218667ecdd3711ab1
https://doi.org/10.1099/0022-1317-67-4-683

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Proteolytic digestion of reovirus in the intestinal lumens of neonatal mice

Autor: Max L. Nibert, D K Bodkin, B N Fields

Publikováno v: Journal of Virology. 63:4676-4681

Two approaches were used to demonstrate proteolysis of reovirus in the intestine of the neonatal mouse. The first approach utilized peroral inoculation of radiolabeled virus into neonatal mice; the intestinal washings were harvested at 0 to 30 min po

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d949df9c02aabe3a87661b887d2760e
https://doi.org/10.1128/jvi.63.11.4676-4681.1989

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Autor: D. K. Bodkin, D. L. Knudson

Publikováno v: The Journal of general virology. 68

The sequence relatedness of ten isolates of the Colorado tick fever (CTF) serogroup of orbiviruses was examined by RNA-RNA blot hybridization. The 12 dsRNA genome segments of each of the isolates were electrophoresed in a 10% polyacrylamide gel, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fabe2c5c5069207438a36661d7de9f9
https://pubmed.ncbi.nlm.nih.gov/3572358

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Biological and antigenic characterization of Netivot virus, an unusual new Orbivirus recovered from mosquitoes in Israel

Autor: Robert B. Tesh, J. Peleg, J. Margalit, D.L. Knudson, I. Samina, D. K. Bodkin, R. E. Shope

Publikováno v: The American journal of tropical medicine and hygiene. 35(2)

The antigenic and biological characteristics of a new Orbivirus, designated Netivot virus, are described. This agent was originally recovered in cultures of the C6/36 clone of Aedes albopictus cells from a pool of Culex pipiens captured in Israel. Ne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10c35b9e3e9b141710ad205b9a4c7d15
https://pubmed.ncbi.nlm.nih.gov/2869707

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Growth and survival of reovirus in intestinal tissue: role of the L2 and S1 genes

Autor: B N Fields, D K Bodkin

Publikováno v: Journal of virology. 63(3)

Reovirus serotype 1 Lang can be recovered in high titer from the intestines of neonatal mice up to day 8 after peroral inoculation. By contrast, reovirus serotype 3 Dearing cannot be recovered from intestinal tissue past day 4 after peroral inoculati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a077b7e4e946fbc80a0fd5d26abc452
https://pubmed.ncbi.nlm.nih.gov/2915380

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