Zobrazeno 1 - 10
of 13
pro vyhledávání: '"D K Apps"'
Publikováno v:
Endocrine research. 26(4)
Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates sh
Publikováno v:
The Biochemical journal. 351(Pt 1)
We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse tran
Publikováno v:
European journal of biochemistry. 232(2)
The kinetics of nucleoside-triphosphate-dependent proton translocation by a vacuolar-type adenosine-triphosphatase have been studied, using the enzyme from bovine chromaffin-granule membranes, purified and reconstituted into proteoliposomes. The reac
Publikováno v:
European biophysics journal : EBJ. 23(4)
A K+ channel was incorporated into voltage-clamped planar lipid bilayers from bovine chromaffin granules and resealed granule membranes ("ghosts"). It was not incorporated from plasma membrane-rich fractions from the adrenal medulla. The channel had
Publikováno v:
Biochemical Journal. 239:77-81
The proton-translocating adenosine triphosphatase (ATPase) of bovine chromaffin granules contains up to five different polypeptides. Its activity is inhibited by N-ethylmaleimide, and ATP protects the enzyme from inhibition. After treatment of membra
Autor:
D K Apps, Angus C. Nairn
Publikováno v:
Biochemical Journal. 167:87-93
The reversibility of the NAD+ kinase reaction was established, and the kinetic parameters of the rate equation in the reverse direction were determined. The equilibrium constant of the reaction was determined by using the purified pigeon liver enzyme
Autor:
D. K. Apps
Publikováno v:
European Journal of Biochemistry. 5:444-450
A procedure for the purification of NAD kinase from pigeon liver acetone powder is described. Although not homogeneous, the preparation is largely free of enzymes which interfere with the assay of NAD kinase. The kinetics with magnesium as the activa
The association of adenosine triphosphatase and ADP/ATP isotope-exchange activities with chromaffin-granule membranes was shown by sucrose-density-gradient centrifugation. The two activities were solubilized, and separated by differential sedimentati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a16dff3b01221b3d7205f7a5dfd406e0
https://europepmc.org/articles/PMC1183652/
https://europepmc.org/articles/PMC1183652/
Autor:
P V Sundaram, D K Apps
Publikováno v:
The Biochemical journal. 161(2)
Pigeon liver NAD kinase was covalently coupled to the inside surfaces of nylon tubes, both directly to the nylon and via polyamine specers Km values and the inactivation energy of the reaction changed upon immobilization, but the pH-dependence remain
Autor:
J H Phillips, D K Apps
Publikováno v:
The Biochemical journal. 192(1)
Catecholamines are accumulated by bovine chromaffin-granule “ghosts” in the presence of MgATP at 25 degrees C. With low concentrations of catecholamine, ratios of internal to external amine concentration of up to 20 000 were obtained. These value