Výsledky vyhledávání

Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger

Autor: D. J. Jeenes, D. B. Archer, C. Ngiam

Publikováno v: Current genetics. 31(2)

Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fcf6a4f6707e6e37a2ccd1c442ad239
https://pubmed.ncbi.nlm.nih.gov/9021130

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A study of D52S hen lysozyme-GlcNAc oligosaccharide complexes by NMR spectroscopy and electrospray mass spectrometry

Autor: K J, Lumb, R T, Aplin, S E, Radford, D B, Archer, D J, Jeenes, N, Lambert, D A, MacKenzie, C M, Dobson, G, Lowe

Publikováno v: FEBS letters. 296(2)

The production of a mutant hen lysozyme is described in which Asp-52, one of the catalytically important residues, is replaced by Ser. The mutant enzyme has very low catalytic activity but NMR studies show that its structure is closely similar to tha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9eaed3854f3c937e2ed0cf2a10e8a71a
https://pubmed.ncbi.nlm.nih.gov/1733771

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Heterologous protein production by filamentous fungi

Autor: D J, Jeenes, D A, Mackenzie, I N, Roberts, D B, Archer

Publikováno v: Biotechnologygenetic engineering reviews. 9

There are clearly many facets to successful production of heterologous proteins from filamentous fungi. The objectives are to exploit the natural ability of some species to secrete high levels of protein. The heterologous target proteins produced in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::107f357aa70f32da438a3f412acc8374
https://pubmed.ncbi.nlm.nih.gov/1839127

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TOL plasmid pWW0 in constructed halobenzoate-degrading Pseudomonas strains: prevention of meta pathway

Autor: P A Williams, H J Knackmuss, W Reineke, D J Jeenes

Publikováno v: Journal of bacteriology. 150(1)

The hybrid pathway for chlorobenzoate metabolism was studied in WR211 and WR216, which were derived from Pseudomonas sp. B13 by acquisition of TOL plasmid pWW0 from Pseudomonas putida mt-2. Chlorobenzoates are utilized readily by these strains when m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::863249e1dfde47174df5343b44afe656
https://pubmed.ncbi.nlm.nih.gov/7061393

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TOL plasmid pWW0 in constructed halobenzoate-degrading Pseudomonas strains: enzyme regulation and DNA structure

Autor: W Reineke, P A Williams, D J Jeenes, H J Knackmuss

WR211 and WR216 are derivatives of halobenzoate-degrading Pseudomonas sp. strain B13 into which the 117-kilobase TOL degradative plasmid pWW0 has been transferred from Pseudomonas putida mt-2. WR211 has lost the ability to grow on the TOL-specific su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::258c0f3f17b1bd3357809eb1619e7b78
https://europepmc.org/articles/PMC220097/

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Excision and integration of degradative pathway genes from TOL plasmid pWW0

Autor: Peter A. Williams, D J Jeenes

WR211 is a transconjugant resulting from transfer of the 117-kilobase (kb) TOL degradative plasmid pWW0 into Pseudomonas sp. strain B13. The plasmid of this strain, pWW01211, is 78 kb long, having suffered a deletion of 39 kb. We show that WR211 cont

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::572089ec272f8a850c469222be0ae1db
https://europepmc.org/articles/PMC220098/

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