Výsledky vyhledávání

Automated Molecular Pathology : One Hour In Situ DNA Hybridization

Autor: Lenora J. Manahan, Chang Soo Park, D. J. Brigati

Publikováno v: Journal of Histotechnology. 14:219-229

We describe a rapid automated technique for colorimetric in situ DNA hybridization using the Code-On Immuno-DNA Slide Stainer. We report recent advances in positively charged glass slide technology, rapid limolene/xylene dewaxing, stable ribonuclease

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fb73584f08c965b8bb9d963c81336da0
https://doi.org/10.1179/his.1991.14.4.219

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Analysis of Epstein-Barr virus in Hodgkin's disease: experience of a single university hospital in Korea

Autor: D. J. Brigati, Chang Soo Park, Kathleen T. Montone, Sang-Woo Juhng

Publikováno v: Journal of clinical laboratory analysis. 8(6)

Hodgkin's disease is known to be associated with Epstein-Barr virus (EBV) infection in Western countries, and viral nucleic acids and proteins have been identified within Reed-Sternberg (RS) cells, which are the histopathologic hallmark of the diseas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81466fa9e882ad73d77cada67a16375e
https://pubmed.ncbi.nlm.nih.gov/7869181

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Immunomycology: rapid and specific immunocytochemical identification of fungi in formalin-fixed, paraffin-embedded material

Autor: B A Hemann, J A Reed, J L Alexander, D J Brigati

Publikováno v: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 41(8)

We report the rapid (less than 1 hr), immunocytochemical identification of various fungi in formalin-fixed, paraffin-embedded tissues using antisera originally developed for use in immunodiffusion assays. Primary antisera directed towards fungal gene

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::677c417963d6bca2f7ead396a28f7a85
https://pubmed.ncbi.nlm.nih.gov/8331285

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A rapid colorimetric in situ messenger RNA hybridization technique for analysis of epidermal growth factor receptor in paraffin-embedded surgical specimens of human colon carcinomas

Autor: R, Radinsky, C D, Bucana, L M, Ellis, R, Sanchez, K R, Cleary, D J, Brigati, I J, Fidler

Publikováno v: Cancer research. 53(5)

We have developed a rapid colorimetric in situ mRNA hybridization procedure to analyze epidermal growth factor receptor (EGF-R) transcripts in paraffin-embedded surgical specimens of human colon carcinomas. This technique is based on the use of 24-ba

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::145e217a9121d05b416ea2f57fb2f540
https://pubmed.ncbi.nlm.nih.gov/8439966

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Complete one-hour immunocytochemistry based on capillary action

Autor: J A, Reed, L J, Manahan, C S, Park, D J, Brigati

Publikováno v: BioTechniques. 13(3)

We describe a less than one-hour manual method for immunocytochemical analyses of B5 or formalin-fixed, paraffin-embedded tissue sections. The method employs capillary action to sequentially apply, incubate and remove liquid reagents from apposed pai

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::adcde2b14524f0766b593cf1a58bb70f
https://pubmed.ncbi.nlm.nih.gov/1389176

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Immunocytochemical identification of Rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia

Autor: L N Slater, J A Reed, D F Welch, N S McNutt, Kyung-Whan Min, D J Brigati, S D Flynn

Publikováno v: The American journal of surgical pathology. 16(7)

We report the immunocytochemical identification of Rochalimaea henselae, a newly recognized fastidious, Gram-negative, Warthin-Starry-positive organism, as the common pathogen in bacillary angiomatosis (BA), bacillary peliosis (BP) of the liver and s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6ce2a8983d7981edb3d0c3efe230f2c
https://pubmed.ncbi.nlm.nih.gov/1530106

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Colorimetric detection of herpes simplex virus by DNA in situ sandwich hybridization: a rapid, formamide-free, random oligomer-enhanced method

Autor: Jin-Han Kang, J. C. Iezzoni, D. J. Brigati, Kathleen T. Montone, J. A. Reed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d2fd955c7f6ca6029d9b7e74f14e1a7
https://europepmc.org/articles/PMC312113/

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Detection of Epstein-Barr virus genomes by in situ DNA hybridization with a terminally biotin-labeled synthetic oligonucleotide probe from the EBV Not I and Pst I tandem repeat regions

Autor: K T, Montone, L R, Budgeon, D J, Brigati

Publikováno v: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 3(1)

We describe a case of acute, disseminated Epstein-Barr virus (EBV) infection which was analyzed for the cellular distribution of viral replication by automated, colorimetric in situ DNA hybridization using a single, synthetic, terminally biotin-label

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::79cd8f72234b51e1c1a411052fc67391
https://pubmed.ncbi.nlm.nih.gov/2155420

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