Zobrazeno 1 - 10
of 40
pro vyhledávání: '"D F, SILBERT"'
Publikováno v:
Journal of Lipid Research, Vol 23, Iss 3, Pp 405-409 (1982)
A series of LM cell variants resistant to filipin, but not auxotrophic for sterol, was isolated by plating mutagenized, filipin-treated cells on soft agar medium containing no sterol. Cloned variants were assayed for growth in the presence and absenc
Externí odkaz:
https://doaj.org/article/c95da8c7e5c64e25adc1d8c84a0b44aa
Autor:
D F Silbert, Mike A. Leonis
Publikováno v:
Journal of Biological Chemistry. 268:9416-9424
We report here further characterization of the Chinese hamster lung fibroblast mutant D1-9b displaying elevated agonist-induced phosphatidylinositol (PI) turnover responses relative to CCL39, the parental cell line (Rath, H. M., Doyle, G. A. R., and
Publikováno v:
The Journal of biological chemistry. 269(34)
A mutant fibroblast, 2A4b, was isolated from the Chinese hamster lung cell line CCL39 by a previously described selection (Rath, H. M., Doyle, G. A. R., and Silbert, D. F. (1989) J. Biol. Chem. 264, 13387-13390) for cells deficient in thrombin-induce
Publikováno v:
The Journal of biological chemistry. 268(11)
Growth of Chinese hamster ovary and LM cells is inhibited by relatively low concentrations of sphingosine in the culture media. This effect is diminished by an order of magnitude by conversion of this positively charged long-chain amino alcohol to a
Publikováno v:
Biochemistry. 29(17)
Deuterium NMR is used to study the selectively labeled Val1...(2-2H)Ala3...gramicidin A molecule to investigate the structure and dynamics of the C alpha-2H bond in the Ala3 residue of gramicidin. Val1...(2-2H)Ala3...gramicidin A is synthesized, puri
Publikováno v:
Biochemistry. 29(17)
Deuterium NMR is used to study the structure and dynamics of the formyl C-2H bond in selectively deuterated gramicidin molecules. Specifically, the functionally different analogues 2HCO-Val1...gramicidin A and 2HCO-Val1-D-Leu2...gramicidin A are stud
Publikováno v:
The Journal of biological chemistry. 265(6)
In a previous paper (Rath, H. M., Doyle, G. A. R., and Silbert, D. F. (1989) J. Biol. Chem. 264, 13387-13390), we reported a selection for the isolation of Chinese hamster lung fibroblasts (CCL39) defective in thrombin-induced mitogenesis. One mutant
Autor:
D. L. A. Greenway, D. F. Silbert
Publikováno v:
Journal of Biological Chemistry. 258:13034-13042
Growth of a temperature-sensitive general fatty acid synthesis mutant of Escherichia coli K12 at its restrictive temperature in the presence of exogenous palmitate results in lysis of the bacterium. Under these conditions, palmitate is incorporated i
Publikováno v:
Journal of Biological Chemistry. 261:7204-7214
Phospholipid-sterol interactions were investigated using parinaric acid fluorescence spectroscopy. Cholesterol and cholesterol analogues which were modified in the sterol nucleus or side chain were added at 50 mol % to multilamellar vesicles of model
Autor:
Y Saito, D F Silbert
Publikováno v:
Journal of Biological Chemistry. 254:1102-1107
Using a sterol-specific auxotroph of LM cells (mouse fibroblasts), we obtained cells with progressively lower sterol contents. 3-O-Methylglucose and thymidine were shown to enter the cells by saturable as well as by nonsaturable processes and it was