Zobrazeno 1 - 10
of 33
pro vyhledávání: '"D E, Hruby"'
Autor:
D E Hruby
Publikováno v:
Clinical Microbiology Reviews. 3:153-170
The development and continued refinement of techniques for the efficient insertion and expression of heterologous DNA sequences from within the genomic context of infectious vaccinia virus recombinants are among the most promising current approaches
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8b02f9d2f7e31578ed8d3265979f57b
https://doi.org/10.1128/cmr.3.2.153
https://doi.org/10.1128/cmr.3.2.153
Publikováno v:
Journal of molecular microbiology and biotechnology. 3(4)
The methods currently employed to produce recombinant Streptococcus gordonii strains for use as vaccines and/or protein expression vectors result in the insertion of foreign genes into an unknown integration site with no information on the transcript
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5923e7b10ebbf3257b810bca922c11c7
https://pubmed.ncbi.nlm.nih.gov/11545273
https://pubmed.ncbi.nlm.nih.gov/11545273
Publikováno v:
Applied and environmental microbiology. 63(6)
This paper describes an immunochemical method which uses radioactive protein A for the detection and analysis of streptococcal M6 protein epitopes on the surface of recombinant Streptococcus gordonii. With this assay, recombinant S. gordonii cells ex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d3032e37334bed396e06872e25ad8a8
https://pubmed.ncbi.nlm.nih.gov/9172373
https://pubmed.ncbi.nlm.nih.gov/9172373
Publikováno v:
The Journal of biological chemistry. 272(3)
Computer-assisted alignment of known palmitylproteins was used to identify a potential peptide motif, TMDX1-12AAC(C)A (TMD, transmembrane domain; X, any amino acid; C, cysteine acceptor residues; A, aliphatic residue) responsible for directing intern
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cf8023924aba042d04539af9b0cd7d1f
https://pubmed.ncbi.nlm.nih.gov/8999886
https://pubmed.ncbi.nlm.nih.gov/8999886
Autor:
P, Lee, D E, Hruby
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 63
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::879fc9ded73384993a6f8ce737485152
https://pubmed.ncbi.nlm.nih.gov/9113638
https://pubmed.ncbi.nlm.nih.gov/9113638
Publikováno v:
Journal of virology. 70(6)
Gel analysis of vaccinia virus particles purified by buoyant [correction of bouyant] density demonstrates a protein with an estimated molecular mass of 59 kDa, which is apparently restricted to the intracellular mature virion (IMV) form. Western blot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a0994dbcf80e2820f336ffab9cc4be5
https://pubmed.ncbi.nlm.nih.gov/8648667
https://pubmed.ncbi.nlm.nih.gov/8648667
Autor:
P, Lee, D E, Hruby
Publikováno v:
The Journal of biological chemistry. 269(11)
Previous studies have suggested that cleavage of vaccinia virus core protein precursors occurs within the consensus tripeptide motif -A-G decreases X-. As an approach to delineate the sequence and structural features of the precursor polypeptides tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e58154f00afb8a6be6322762b5285bc4
https://pubmed.ncbi.nlm.nih.gov/8132587
https://pubmed.ncbi.nlm.nih.gov/8132587
Autor:
D E, Hruby
Publikováno v:
Seminars in hematology. 30(4 Suppl 4)
One of the more promising new approaches for producing prophylactic vaccines involves the genetic engineering of vaccinia virus (VV) strains to express a single protein, a portion of a protein, or a single epitope from a heterologous pathogen. As an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ff1393674f5d149003c7dc46259c0aae
https://pubmed.ncbi.nlm.nih.gov/8303309
https://pubmed.ncbi.nlm.nih.gov/8303309
Publikováno v:
The Journal of biological chemistry. 268(10)
The vaccinia virus L1R gene product is a late protein destined for insertion into the envelope of intracellular virus particles. Because this protein is co-translationally modified by the addition of myristic acid to the penultimate NH2-terminal glyc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c1a819a743f90a190b6fb84fabcc6a2d
https://pubmed.ncbi.nlm.nih.gov/8463289
https://pubmed.ncbi.nlm.nih.gov/8463289
Autor:
M E, Black, D E, Hruby
Publikováno v:
The Journal of biological chemistry. 267(10)
Alignment of prokaryotic and vertebrate type II thymidine kinases (TK) (EC 2.7.1.21), such as that encoded by vaccinia virus (VVTK), reveals three conserved regions: designated domains I, III, and VII. Domains I and III of VVTK contain residues which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ff8f1f7c98195c6f6f7a043c770c1388
https://pubmed.ncbi.nlm.nih.gov/1551887
https://pubmed.ncbi.nlm.nih.gov/1551887