Zobrazeno 1 - 10
of 23
pro vyhledávání: '"D E, Epps"'
Publikováno v:
Journal of Lipid Research, Vol 26, Iss 1, Pp 135-140 (1985)
A method utilizing aminopropyl bonded phase (Bond Elut) columns has been developed to separate lipid mixtures into individual classes in high yield and purity. Up to ten lipid mixtures can be processed in 1 hr and the columns are reusable after suita
Externí odkaz:
https://doaj.org/article/7d3a0d365c3849598fe7c47714cb6787
Publikováno v:
Journal of Lipid Research, Vol 27, Iss 7, Pp 792-795 (1988)
A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-asso
Externí odkaz:
https://doaj.org/article/e61df251894d4d7b8a738d2fd7b2faad
Publikováno v:
Cytokine. 9:149-156
The authors mutated two key residues in the sequence of the cytokine interleukin 1β, namely the double mutant Phe46 to Trp46 and Trp120 to Phe120 and the single point mutation Lys103 to Leu103 and measured the resulting receptor binding and biologic
Autor:
K.A. Greenlee, E W Thomas, C.K. Marschke, R R Hozak, George W. Melchior, Jed F. Fisher, Christine Castle, D E Epps, Ferenc J. Kezdy, J.S. Harris
Publikováno v:
Biochemistry. 34:12560-12569
The cholesteryl ester transfer protein-catalyzed cholesteryl ester transfer is inhibited by two compounds identified by a large-scale screening of cholesterol backbone-containing molecules. Kinetic analysis shows that U-95,594, an amino steroid, inhi
Publikováno v:
Journal of Biological Chemistry. 267:3129-3135
We have determined the fluorescence properties of two covalently attached acrylodan derivatives of recombinant human interleukin-1 beta, namely the Cys-8 and Lys-103 adducts. The emission and excitation maxima indicated the presence of two operationa
Autor:
D E Epps, N. D. Staite, Martin R. Deibel, W R Mathews, Karen A. Richard, David M. Guido, Anthony W. Yem
Publikováno v:
Journal of Biological Chemistry. 267:3122-3128
Acrylodan, which normally modifies cysteine residues, was employed to derivatize recombinant interleukin-1 beta (rIL-1 beta) under native conditions, using a reagent:protein ratio of 3:1. Two major covalent protein/acrylodan adducts were generated an
Publikováno v:
Journal of protein chemistry. 17(7)
The active site of the catalytic domain of stromelysin-1 (matrix metalloproteinase-3, MMP-3) was probed by fluorescence quenching, lifetime, and polarization of its three intrinsic tryptophans and by the environmentally sensitive fluorescent reporter
Publikováno v:
The Journal of biological chemistry. 267(5)
We have determined the fluorescence properties of two covalently attached acrylodan derivatives of recombinant human interleukin-1 beta, namely the Cys-8 and Lys-103 adducts. The emission and excitation maxima indicated the presence of two operationa
Autor:
Tomi K. Sawyer, Cheney J, Prairie M, William C. Krueger, Heinrich J. Schostarez, D. E. Epps, F. Mandel
Publikováno v:
Journal of medicinal chemistry. 33(8)
The independent subsite model is widely used for the design of peptide inhibitors of enzymes with extended active sites. This model assumes that the subsites are independent of each other and that the free energies of binding contributed by the sever
Publikováno v:
Journal of Lipid Research, Vol 27, Iss 7, Pp 792-795 (1988)
A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-asso