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Publikováno v:
Gene. 382:47-56
Exposure of cells to ionizing radiation (IR) produces changes in the expression level of a large number of genes. However, less is known of gene-expression changes caused by local radiation exposure from radionuclides within cells. We studied changes
Autor:
Vladislav A. Malkov, Ronald D. Neumann, R D Camerini-Otero, Igor G. Panyutin, Victor B. Zhurkin
Publikováno v:
Journal of Molecular Biology. 299:629-640
A fundamental problem in homologous recombination is how homology between DNAs is recognized. In all current models, a recombination protein loads onto a single strand of DNA and scans another duplex for homology. When homology is found, a synaptic c
Publikováno v:
Protein Science. 9:2161-2169
The Escherichia coli RecA protein triggers both DNA repair and mutagenesis in a process known as the SOS response. The 81-residue E. coli protein DinI inhibits activity of RecA in vivo. The solution structure of DinI has been determined by multidimen
Publikováno v:
Journal of Molecular Biology. 286:1097-1106
The disordered mobile loop L2 of the Escherichia coli RecA protein is known to play a central role in DNA binding and pairing. To investigate the local chemical environment in relation to function we performed saturation mutagenesis of the loop L2 re
Publikováno v:
Journal of Molecular Biology. 278:317-330
We determined that the incorporation of one mismatch into RecA mediated synaptic complexes between oligonucleotide single-stranded DNAs and target duplex DNAs destabilizes the complex by 0.8 to 1.9 kcal/mol. This finding supports our previous result,
Autor:
R D Camerini-Otero, L J Ferrin
Publikováno v:
Proceedings of the National Academy of Sciences. 95:2152-2157
A method is described that allows the sequence-specific ligation of DNA. The method is based on the ability of RecA protein from Escherichia coli to selectively pair oligonucleotides to their homologous sequences at the ends of fragments of duplex DN
Publikováno v:
Journal of Molecular Biology. 271:168-177
We have developed an in vitro selection procedure to elucidate the specificity of RecA assisted oligonucleotide recognition of double stranded DNA. The procedure was based on formation of a synaptic complex between an oligonucleotide-RecA filament an
Autor:
P Hsieh, R D Camerini-Otero
Publikováno v:
Annual Review of Genetics. 29:509-552
Genetic recombination is common to all forms of life and involves the exchange of DNA sequences between two chromosomes or DNA molecules. Such exchanges contribute to the generation of genetic diversity and the repair of damaged DNA. There are two ma
The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization
Autor:
R D Camerini-Otero, E Angov
Publikováno v:
Journal of Bacteriology. 176:1405-1412
We have cloned, expressed, and purified the RecA analog from the thermophilic eubacterium Thermus aquaticus YT-1. Analysis of the deduced amino acid sequence indicates that the T. aquaticus RecA is structurally similar to the Escherichia coli RecA an