Exportins can inhibit major mitotic assembly events in vitro: membrane fusion, nuclear pore formation, and spindle assembly

Autor: Anna Travesa, Sarah Carmona, Dennis C. Garland, Cyril Bernis, Matthew S Nord, Douglass J. Forbes

Publikováno v: Nucleus, Vol 11, Iss 1, Pp 178-193 (2020)
Nucleus
article-version (VoR) Version of Record

Xenopus egg extracts are a powerful in vitro tool for studying complex biological processes, including nuclear reconstitution, nuclear membrane and pore assembly, and spindle assembly. Extracts have been further used to demonstrate a moonlighting reg

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c58c6507ea8dffca89ff299818fb7ee9
https://doi.org/10.1080/19491034.2020.1798093

Transportin acts to regulate mitotic assembly events by target binding rather than Ran sequestration

Autor: Yuh Min Chook, Douglass J. Forbes, Sarah Carmona, Beth Swift-Taylor, Cyril Bernis, Matthew S Nord

Publikováno v: Molecular Biology of the Cell

Transportin-specific molecular tools are used to show that the mitotic cell contains importin β and transportin “global positioning system” pathways that are mechanistically parallel. Transportin works to control where the spindle, nuclear membr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e492bb53df703e5e8e92a2d0104f4c6
http://europepmc.org/articles/PMC3967982

Nuclear transport factors: global regulation of mitosis

Autor: Cyril Bernis, Anna Travesa, Matthew S Nord, Douglass J. Forbes

The unexpected repurposing of nuclear transport proteins from their function in interphase to an equally vital and very different set of functions in mitosis was very surprising. The multi-talented cast when first revealed included the import recepto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db2dcf74d1a110a8b811a584b966d430
https://escholarship.org/uc/item/0b44d3r6

Kinetochore Localization of Spindle Checkpoint Proteins: Who Controls Whom?

Autor: Thierry Lorca, Cyril Bernis, Anna Castro, Susana Prieto, Jean-Claude Labbé, Suzanne Vigneron

Publikováno v: Molecular Biology of the Cell
Molecular Biology of the Cell, American Society for Cell Biology, In press, 15 (10), pp.4584-4596. ⟨10.1091/mbc.e04-01-0051⟩

International audience; The spindle checkpoint prevents anaphase onset until all the chromosomes have successfully attached to the spindle microtubules. The mechanisms by which unattached kinetochores trigger and transmit a primary signal are poorly

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::047266130e761d2f52f3bb0ee5567287
https://doi.org/10.1091/mbc.e04-01-0051

Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly

Autor: Douglass J. Forbes, Quang Phung, Corine K. Lau, Beth A. Rasala, Boris Fichtman, Valerie A. Delmar, Rene C. Chan, Cyril Bernis

Publikováno v: Molecular biology of the cell. 20(18)

Mitosis in higher eukaryotes is marked by the sequential assembly of two massive structures: the mitotic spindle and the nucleus. Nuclear assembly itself requires the precise formation of both nuclear membranes and nuclear pore complexes. Previously,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e686de8d93c4d777c6ad53a9759559d8
https://pubmed.ncbi.nlm.nih.gov/19641022