An unbiased cell morphology-based screen for new, biologically active small molecules.

Autor: Masahiro Tanaka, Raynard Bateman, Daniel Rauh, Eugeni Vaisberg, Shyam Ramachandani, Chao Zhang, Kirk C Hansen, Alma L Burlingame, Jay K Trautman, Kevan M Shokat, Cynthia L Adams

Publikováno v: PLoS Biology, Vol 3, Iss 5, p e128 (2005)

We have implemented an unbiased cell morphology-based screen to identify small-molecule modulators of cellular processes using the Cytometrix (TM) automated imaging and analysis system. This assay format provides unbiased analysis of morphological ef

Externí odkaz: https://doaj.org/article/793028f6ead843649a23bfd14fe7747d

Design and Implementation of High Content Imaging Platforms: Lessons Learned from End User – Developer Collaboration

Autor: Cynthia L Adams, Michael D Sjaastad

Publikováno v: Combinatorial Chemistry & High Throughput Screening. 12:877-887

Automated high-content screening and analysis (HCS/HCA) technology solutions have become indispensable in expediting the pace of drug discovery. Because of the complexity involved in designing, building, and validating HCS/HCA platforms, it is import

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::324f5d3eca8268213a63240d0a85690a
https://doi.org/10.2174/138620709789383240

Compound classification using image-based cellular phenotypes

Autor: Cynthia L, Adams, Vadim, Kutsyy, Daniel A, Coleman, Ge, Cong, Anne Moon, Crompton, Kathleen A, Elias, Donald R, Oestreicher, Jay K, Trautman, Eugeni, Vaisberg

Publikováno v: Methods in enzymology. 414

Compounds with similar target specificities and modes of inhibition cause similar cellular phenotypes. Based on this observation, we hypothesized that we could quantitatively classify compounds with diverse mechanisms of action using cellular phenoty

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::eabc13c78e8520fced990475ea434b48
https://pubmed.ncbi.nlm.nih.gov/17110206

Automated Imaging Screen Reveals Promising Drug Candidates

Autor: Daniel Rauh, Eugeni A. Vaisberg, Shyam Ramachandani, Cynthia L. Adams, Raynard L. Bateman, Kirk C. Hansen, Jay Trautman, Chao Zhang, Alma L. Burlingame, Masahiro Tanaka, Kevan M. Shokat

Publikováno v: PLoS Biology, Vol 3, Iss 5, p e128 (2005)
Tanaka, M; Bateman, R; Rauh, D; Vaisberg, E; Ramachandani, S; Zhang, C; et al.(2005). An unbiased cell morphology-based screen for new, biologically active small molecules.. PLoS biology., 3(5), e128. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/5qm1k6cx
PLoS Biology
PLoS biology, vol 3, iss 5

We have implemented an unbiased cell morphology–based screen to identify small-molecule modulators of cellular processes using the Cytometrix (TM) automated imaging and analysis system. This assay format provides unbiased analysis of morphological

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9cdbc9cb93bba09b6743cad15ba513b
http://europepmc.org/articles/PMC1073692?pdf=render

New Methodologies in the Preparation of Peptide Libraries

Autor: Richard B. Mortensen, Lynn M. Kochersperger, Lois A. Aldwin, Cynthia L. Adams, Christopher P. Holmes

Publisher Summary This chapter describes new methodologies in the preparation of peptide libraries. The photochemical synthesis of peptides is facilitated through the use of nitroveratryloxycarbonyl (Nvoc) as the protecting group for the terminal ami

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c9c593f33f72802f1ba18ed28e228277
https://doi.org/10.1016/b978-0-12-194710-1.50064-3