IQCELL: A platform for predicting the effect of gene perturbations on developmental trajectories using single-cell RNA-seq data.

Autor: Tiam Heydari, Matthew A Langley, Cynthia L Fisher, Daniel Aguilar-Hidalgo, Shreya Shukla, Ayako Yachie-Kinoshita, Michael Hughes, Kelly M McNagny, Peter W Zandstra

Publikováno v: PLoS Computational Biology, Vol 18, Iss 2, p e1009907 (2022)

The increasing availability of single-cell RNA-sequencing (scRNA-seq) data from various developmental systems provides the opportunity to infer gene regulatory networks (GRNs) directly from data. Herein we describe IQCELL, a platform to infer, simula

Externí odkaz: https://doaj.org/article/ac7a7750987c4461be0be648a96584c1

A microfluidic device for characterizing nuclear deformations

Autor: Andrew C Hodgson, Kevin J. Chalut, Christophe M. Verstreken, Stefano Pagliara, Cynthia L. Fisher, Ulrich F. Keyser

Cell nuclei experience and respond to a wide range of forces, both in vivo and in vitro. In order to characterize the nuclear response to physical stress, we developed a microfluidic chip and used it to apply mechanical stress to live cells and measu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7b75a416c441a447bd466ee109781b1

The interaction of PRC2 with RNA or chromatin is mutually antagonistic

Autor: Cynthia L. Fisher, Keijo Viiri, Marcus Dawson, Richard G. Jenner, Jernej Ule, Benjamin M. Foster, Christopher R. Sibley, Till Bartke, Christopher M. Yates, Lenka Skalska, Manuel Beltran, Filipa P. Reis

Publikováno v: Beltran, M, Yates, C M, Skalska, L, Dawson, M, Reis, F P, Viiri, K, Fisher, C L, Sibley, C R, Foster, B M, Bartke, T, Ule, J & Jenner, R G 2016, ' The interaction of PRC2 with RNA or chromatin is mutually antagonistic ', Genome Research, vol. 26, no. 7, pp. 896-907 . https://doi.org/10.1101/gr.197632.115

Polycomb repressive complex 2 (PRC2) modifies chromatin to maintain genes in a repressed state during development. PRC2 is primarily associated with CpG islands at repressed genes and also possesses RNA binding activity. However, the RNAs that bind P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf6af2b84141bd603b63bc8aaf2de55d
https://www.pure.ed.ac.uk/ws/files/110240556/Genome_Res._2016_Beltran_896_907.pdf

Additional sex combs-like 1 belongs to the enhancer of trithorax and polycomb group and genetically interacts with Cbx2 in mice

Autor: J. Chevalier, R.K. Humphries, C. Bodner, Cynthia L. Fisher, Jay L. Hess, A. Dahl, Cheryl D. Helgason, I. Lee, Hugh W. Brock, S. Bozza, S. Bloyer

Publikováno v: Developmental Biology. 337:9-15

The Additional sex combs (Asx) gene of Drosophila behaves genetically as an enhancer of trithorax and polycomb (ETP) in displaying bidirectional homeotic phenotypes, suggesting that is required for maintenance of both activation and silencing of Hox

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6daf6174bcbf780cd6bf78498f82a94c
https://doi.org/10.1016/j.ydbio.2009.10.004

Characterization of ABCB9, an ATP Binding Cassette Protein Associated with Lysosomes

Autor: Sarah J. Childs, Lin Liu, Cynthia L. Fisher, Victor Ling, Katerina Dorovini-Zis, Wandong Zhang, David Hui, Fang Zhang

Publikováno v: Journal of Biological Chemistry. 275:23287-23294

We have cloned full-length human and mouse cDNAs of ABCB9, which encodes a predicted multiple-spanning transmembrane domain and a nucleotide-binding domain with Walker motifs. It is therefore designated as a "half" ATP binding cassette (ABC) transpor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8615461e1473e7571cb60cd7db5b1a2c
https://doi.org/10.1074/jbc.m001819200