The structural basis for monoclonal antibody 5D2 binding to the tryptophan-rich loop of lipoprotein lipase

Autor: John G. Luz, Anne P. Beigneux, DeeAnn K. Asamoto, Cuiwen He, Wenxin Song, Christopher M. Allan, Jazmin Morales, Yiping Tu, Adam Kwok, Thomas Cottle, Muthuraman Meiyappan, Loren G. Fong, Judy E. Kim, Michael Ploug, Stephen G. Young, Gabriel Birrane

Publikováno v: Journal of Lipid Research, Vol 61, Iss 10, Pp 1347-1359 (2020)

For three decades, the LPL–specific monoclonal antibody 5D2 has been used to investigate LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL levels, block the triglyceride hydrolase activity of LPL, and prevent the

Externí odkaz: https://doaj.org/article/a080c819825b4d84b63608de28505ec2

Monoclonal antibodies that bind to the Ly6 domain of GPIHBP1 abolish the binding of LPL

Autor: Xuchen Hu, Mark W. Sleeman, Kazuya Miyashita, MacRae F. Linton, Christopher M. Allan, Cuiwen He, Mikael Larsson, Yiping Tu, Norma P. Sandoval, Rachel S. Jung, Alaleh Mapar, Tetsuo Machida, Masami Murakami, Katsuyuki Nakajima, Michael Ploug, Loren G. Fong, Stephen G. Young, Anne P. Beigneux

Publikováno v: Journal of Lipid Research, Vol 58, Iss 1, Pp 208-215 (2017)

GPIHBP1, an endothelial cell protein, binds LPL in the interstitial spaces and shuttles it to its site of action inside blood vessels. For years, studies of human GPIHBP1 have been hampered by an absence of useful antibodies. We reasoned that monoclo

Externí odkaz: https://doaj.org/article/2cd466d9dd7b46b5858c18eb7905f80f

GPIHBP1 expression in gliomas promotes utilization of lipoprotein-derived nutrients

Autor: Xuchen Hu, Ken Matsumoto, Rachel S Jung, Thomas A Weston, Patrick J Heizer, Cuiwen He, Norma P Sandoval, Christopher M Allan, Yiping Tu, Harry V Vinters, Linda M Liau, Rochelle M Ellison, Jazmin E Morales, Lynn J Baufeld, Nicholas A Bayley, Liqun He, Christer Betsholtz, Anne P Beigneux, David A Nathanson, Holger Gerhardt, Stephen G Young, Loren G Fong, Haibo Jiang

Publikováno v: eLife, Vol 8 (2019)

GPIHBP1, a GPI-anchored protein of capillary endothelial cells, binds lipoprotein lipase (LPL) within the subendothelial spaces and shuttles it to the capillary lumen. GPIHBP1-bound LPL is essential for the margination of triglyceride-rich lipoprotei

Externí odkaz: https://doaj.org/article/c9e3de5bd1f74f16b0b5035d702b97d3

High-resolution visualization and quantification of nucleic acid–based therapeutics in cells and tissues using Nanoscale secondary ion mass spectrometry (NanoSIMS)

Autor: Punit P. Seth, Haibo Jiang, Stephen G. Young, K. Swaminathan Iyer, Paul Guagliardo, Cuiwen He, Kai Chen, Thomas A. Weston, Loren G. Fong, Michael T. Migawa, C. Frank Bennett, Wenxin Song, Michael Tanowitz

Publikováno v: Nucleic Acids Research

Nucleic acid therapeutics (NATs) have proven useful in promoting the degradation of specific transcripts, modifying gene expression, and regulating mRNA splicing. In each situation, efficient delivery of nucleic acids to cells, tissues and intracellu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::397731af11f124b15e1a8b566724f089
https://doi.org/10.1093/nar/gkaa1112

The structural basis for monoclonal antibody 5D2 binding to the tryptophan-rich loop of lipoprotein lipase

Autor: Stephen G. Young, Michael Ploug, Thomas Cottle, Christopher M. Allan, Yiping Tu, DeeAnn K. Asamoto, Judy E. Kim, Wenxin Song, Muthuraman Meiyappan, Loren G. Fong, Cuiwen He, Jazmin E. Morales, Gabriel Birrane, Adam Kwok, J.G. Luz, Anne P. Beigneux

Publikováno v: Journal of lipid research, vol 61, iss 10
Journal of Lipid Research, Vol 61, Iss 10, Pp 1347-1359 (2020)
Luz, J, Beigneux, A P, Asamoto, D K, He, C, Song, W, Allan, C M, Morales, J E, Tu, Y, Kwok, A, Cottle, T, Meiyappan, M, Fong, L G, Kim, J, Ploug, M, Young, S G & Birrane, G 2020, ' The structural basis for monoclonal antibody 5D2 binding to the tryptophan-rich loop of lipoprotein lipase ', Journal of Lipid Research, vol. 61, no. 10, pp. 1347-1359 . https://doi.org/10.1194/jlr.RA120000993
J Lipid Res

For three decades, the lipoprotein lipase (LPL)-specific monoclonal antibody 5D2 has been used to investigate LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL levels, block the triglyceride hydrolase activity of LP

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3ee1923dc4ca97066b417156ef0ae5b
https://escholarship.org/uc/item/03p650j9