Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Crystal M. Han"'
Autor:
Crystal M. Han, Ruba Khnouf
Publikováno v:
IEEE Nanotechnology Magazine. 14:6-17
Protein detection and quantification is a crucial part of many clinical, research, and preparatory applications [1]. Protein biomarkers, such as prostate-specific antigen (PSA) [2] and carcinoembryonic antigen (CEA), are regularly detected and quanti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cf37646c38763735fc862c157b1c540e
https://doi.org/10.1109/mnano.2020.2966028
https://doi.org/10.1109/mnano.2020.2966028
Simultaneous RNA purification and size selection using on-chip isotachophoresis with an ionic spacer
Autor:
Crystal M. Han, Ruba Khnouf, Sarah A. Munro, Marc L. Salit, Michael Snyder, David Catoe, Juan G. Santiago, Can Cenik
Publikováno v:
Lab Chip
We present an on-chip method for the extraction of RNA within a specific size range from low-abundance samples. We use isotachophoresis (ITP) with an ionic spacer and a sieving matrix to enable size-selection with a high yield of RNA in the target si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5285ab540e0a97cda080dc70b95565d
https://doi.org/10.1039/c9lc00311h
https://doi.org/10.1039/c9lc00311h
Autor:
Crystal M. Han, Anton P. McCaffrey, Sabrina Shore, Ruba Khnouf, Jordana M. Henderson, Hirofumi Shintaku, Juan G. Santiago, Sarah A. Munro
Publikováno v:
Analytical Chemistry
Although single-cell mRNA sequencing has been a powerful tool to explore cellular heterogeneity, the sequencing of small RNA at the single-cell level (sc-sRNA-seq) remains a challenge, as these have no consensus sequence, are relatively low abundant,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3486e494a5c72569225cc53b4842cde
https://doi.org/10.1021/acs.analchem.8b02773
https://doi.org/10.1021/acs.analchem.8b02773
Publikováno v:
Electrophoresis5 References. 40(23-24)
In spite of the growing interest in the roles and applications of small RNAs (sRNAs), sRNA isolation methods are inconsistent, tedious, and dependent on the starting number of cells. In this work, we employ ITP to isolate sRNAs from the cell-lysate o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cf577d34c802258826814997e2cf7b1
https://pubmed.ncbi.nlm.nih.gov/31675123
https://pubmed.ncbi.nlm.nih.gov/31675123
Autor:
Juan G. Santiago, Sergiu P. Paşca, Sergio Leal-Ortiz, Joseph C. Wu, Haodong Chen, Yuhong Cao, Nicholas A. Melosh, Fikri Birey, Crystal M. Han, Martin Hjort
Publikováno v:
Proceedings of the National Academy of Sciences. 114
Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::001869c635325841754d8cac4ea95035
https://doi.org/10.1073/pnas.1615375114
https://doi.org/10.1073/pnas.1615375114
Publikováno v:
Lab Chip. 14:2958-2967
We present an on-chip electrokinetic method to increase the reaction kinetics and sensitivity of DNA microarray hybridization. We use isotachophoresis (ITP) to preconcentrate target molecules in solution and transport them over the immobilized probe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b4d75bace4e43a085c0c859515b04e0
https://doi.org/10.1039/c4lc00374h
https://doi.org/10.1039/c4lc00374h
Publikováno v:
Proceedings of the National Academy of Sciences. 109:11127-11132
We use isotachophoresis (ITP) to control and increase the rate of nucleic acid hybridization reactions in free solution. We present a new physical model, validation experiments, and demonstrations of this assay. We studied the coupled physicochemical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6491cb242b9a881bee5455e514d4e93
https://doi.org/10.1073/pnas.1205004109
https://doi.org/10.1073/pnas.1205004109
Publikováno v:
Analytical Chemistry. 83:9715-9718
We demonstrate a technique for purification of nucleic acids from malaria parasites infecting human erythrocytes using isotachophoresis (ITP). We release nucleic acids from malaria-infected erythrocytes by lysing with heat and proteinase K for 10 min
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0251208a5ddde8b1904c93064cadecfd
https://doi.org/10.1021/ac202567j
https://doi.org/10.1021/ac202567j
Autor:
Kathleen E. Mach, Moran Bercovici, Joseph C. Liao, Juan G. Santiago, Govind V. Kaigala, Crystal M. Han
Publikováno v:
Analytical Chemistry. 83:4110-4117
We present a novel assay for rapid detection and identification of bacterial urinary tract infections using isotachophoresis (ITP) and molecular beacons. We applied on-chip ITP to extract and focus 16S rRNA directly from bacterial lysate and used mol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a54b0d0fde46cbf4fa48446ea7f80da4
https://doi.org/10.1021/ac200253x
https://doi.org/10.1021/ac200253x
Publikováno v:
The Analyst. 138(1)
We present a method for rapid, sequence-specific detection of multiple DNA fragments by integrating isotachophoresis (ITP) based DNA hybridization and capillary zone electrophoresis (CZE) using bidirectional ITP. Our method leverages the high preconc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05bb8334685f5d5b2282f5b83cba7980
https://pubmed.ncbi.nlm.nih.gov/23103998
https://pubmed.ncbi.nlm.nih.gov/23103998