Zobrazeno 1 - 10
of 1 195
pro vyhledávání: '"CryoEM"'
Publikováno v:
IUCrJ, Vol 11, Iss 4, Pp 494-501 (2024)
In the folded state, biomolecules exchange between multiple conformational states crucial for their function. However, most structural models derived from experiments and computational predictions only encode a single state. To represent biomolecules
Externí odkaz:
https://doaj.org/article/0b2b761acf384d09b28475fb4cdafe9d
Publikováno v:
BioChem, Vol 4, Iss 2, Pp 62-89 (2024)
Heat Shock Protein 90 (Hsp90) acts as a crucial molecular chaperone, playing an essential role in activating numerous signaling proteins. The intricate mechanism of Hsp90 involving ATPase-coupled conformational changes and interactions with cochapero
Externí odkaz:
https://doaj.org/article/384216e9d1224df39d4dcef17847d5d1
Autor:
Gerard J. Kleywegt, Paul D. Adams, Sarah J. Butcher, Catherine L. Lawson, Alexis Rohou, Peter B. Rosenthal, Sriram Subramaniam, Maya Topf, Sanja Abbott, Philip R. Baldwin, John M. Berrisford, Gérard Bricogne, Preeti Choudhary, Tristan I. Croll, Radostin Danev, Sai J. Ganesan, Timothy Grant, Aleksandras Gutmanas, Richard Henderson, J. Bernard Heymann, Juha T. Huiskonen, Andrei Istrate, Takayuki Kato, Gabriel C. Lander, Shee-Mei Lok, Steven J. Ludtke, Garib N. Murshudov, Ryan Pye, Grigore D. Pintilie, Jane S. Richardson, Carsten Sachse, Osman Salih, Sjors H. W. Scheres, Gunnar F. Schroeder, Carlos Oscar S. Sorzano, Scott M. Stagg, Zhe Wang, Rangana Warshamanage, John D. Westbrook, Martyn D. Winn, Jasmine Y. Young, Stephen K. Burley, Jeffrey C. Hoch, Genji Kurisu, Kyle Morris, Ardan Patwardhan, Sameer Velankar
Publikováno v:
IUCrJ, Vol 11, Iss 2, Pp 140-151 (2024)
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, m
Externí odkaz:
https://doaj.org/article/0e43f020fe4a4894b6f5aa6a5fd3dc2f
Autor:
P. Zambon
Publikováno v:
Frontiers in Physics, Vol 12 (2024)
Coincidence loss can have detrimental effects on the image quality provided by pixelated counting detectors, especially in dose-sensitive applications like cryoEM where the information extracted from the recorded signal needs to be maximized. In this
Externí odkaz:
https://doaj.org/article/b9104774ba0548c5ba5c23129ec74237
Publikováno v:
Advanced Science, Vol 11, Iss 23, Pp n/a-n/a (2024)
Abstract Quantitative analysis of complex mixtures, including compounds having similar chemical properties, is demonstrated using an automatic and high throughput approach to microcrystal electron diffraction (MicroED). Compositional analysis of orga
Externí odkaz:
https://doaj.org/article/81f681c703b346feb17bb71886282940
Autor:
Jesús G. Galaz-Montoya
Publikováno v:
Frontiers in Molecular Biosciences, Vol 11 (2024)
Advances in cryogenic electron microscopy (cryoEM) single particle analysis have revolutionized structural biology by facilitating the in vitro determination of atomic- and near-atomic-resolution structures for fully hydrated macromolecular complexes
Externí odkaz:
https://doaj.org/article/eec0ec8adc14446eaee49ffc74d3466d
Autor:
Bing Liang Alvin Chew, AN Qi Ngoh, Wint Wint Phoo, Kitti Wing Ki Chan, Zheng Ser, Nikhil K Tulsian, Shiao See Lim, Mei Jie Grace Weng, Satoru Watanabe, Milly M Choy, Jenny Low, Eng Eong Ooi, Christiane Ruedl, Radoslaw M Sobota, Subhash G Vasudevan, Dahai Luo
Publikováno v:
eLife, Vol 12 (2024)
Severe dengue infections are characterized by endothelial dysfunction shown to be associated with the secreted nonstructural protein 1 (sNS1), making it an attractive vaccine antigen and biotherapeutic target. To uncover the biologically relevant str
Externí odkaz:
https://doaj.org/article/7d2b25b34b2d403fb3d814958e7d8555
Publikováno v:
Frontiers in Molecular Biosciences, Vol 11 (2024)
The application of machine learning to cryogenic electron microscopy (cryoEM) data analysis has added a valuable set of tools to the cryoEM data processing pipeline. As these tools become more accessible and widely available, the implications of thei
Externí odkaz:
https://doaj.org/article/023944c32f744eef934ce3d2dc1f4d59
Publikováno v:
eLife, Vol 12 (2024)
The dimeric two-pore OSCA/TMEM63 family has recently been identified as mechanically activated ion channels. Previously, based on the unique features of the structure of OSCA1.2, we postulated the potential involvement of several structural elements
Externí odkaz:
https://doaj.org/article/6b02f796555742be94f58f6e2a2a0086
Autor:
J. M. Short, C. M. Palmer, T. Burnley, M. D. Winn, Q. Zhang, B. V. Venkataram Prasad, S. Chen, R. A. Crowther, P. N. T. Unwin, R. Henderson
Publikováno v:
IUCrJ, Vol 10, Iss 5, Pp 579-583 (2023)
The great success of single-particle electron cryo-microscopy (cryoEM) during the last decade has involved the development of powerful new computer programs and packages that guide the user along a recommended processing workflow, in which the wisdom
Externí odkaz:
https://doaj.org/article/d5360841ca404cadb24876836bdeaa22