Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Cristina Parola"'
Autor:
Theresa Pesch, Lucia Bonati, William Kelton, Cristina Parola, Roy A. Ehling, Lucia Csepregi, Daisuke Kitamura, Sai T. Reddy
Publikováno v:
Frontiers in Immunology, Vol 10 (2019)
Immune cell therapies based on the integration of synthetic antigen receptors comprise a powerful strategy for the treatment of diverse diseases, most notably T cells engineered to express chimeric antigen receptors (CAR) for targeted cancer therapy.
Externí odkaz:
https://doaj.org/article/596ae68be7814c0483eaad7d542fb955
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
Hybridomas are widely used for antibody screening and production due to their genetic stability and rapid proliferation. Here the authors demonstrate the rapid reprogramming of antibody specificity in hybridomas using CRISPR-Cas9.
Externí odkaz:
https://doaj.org/article/6925af93eca14d3ca5ad753540c1ac3f
Autor:
Sai T. Reddy, Lucia Csepregi, Tarik A. Khan, Lena Erlach, Cristina Parola, Arthur R. Gorter de Vries, Derek M Mason, Simon Friedensohn, Daniel Neumeier
SUMMARYAdaptive immunity is driven by the ability of lymphocytes to undergo V(D)J recombination and generate a highly diverse set of immune receptors (B cell receptors/secreted antibodies and T cell receptors) and their subsequent clonal selection an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::322e7d43bd00b3801cbc83a6042f98f9
Publikováno v:
Immunology
Monoclonal antibody discovery and engineering is a field that has traditionally been dominated by high‐throughput screening platforms (e.g. hybridomas and surface display). In recent years the emergence of high‐throughput sequencing has made it p
Autor:
Lucia Bonati, Cristina Parola, William Kelton, Theresa Pesch, Roy A. Ehling, Sai T. Reddy, Lucia Csepregi, Daisuke Kitamura
Publikováno v:
Frontiers in Immunology
Frontiers in Immunology, 10
Frontiers in Immunology, 10
Immune cell therapies based on the integration of synthetic antigen receptors comprise a powerful strategy for the treatment of diverse diseases, most notably T cells engineered to express chimeric antigen receptors (CAR) for targeted cancer therapy.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a6b715f9b2ba99d113b1994cc491f37
Autor:
Sai T. Reddy, Lucia Csepregi, Cristina Parola, Daniel Neumeier, Derek M Mason, Simon Friedensohn, Mariangela Di Tacchio
Publikováno v:
mAbs, 11 (8)
mAbs
mAbs
Antibody engineering in mammalian cells offers the important advantage of expression and screening of libraries in their native conformation, increasing the likelihood of generating candidates with more favorable molecular properties. Major advances
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5434e7b60f5be8e327f84ce19d9c2d5
https://hdl.handle.net/20.500.11850/367967
https://hdl.handle.net/20.500.11850/367967
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1850
From the perspective of academic and small research laboratories, the most common and practical strategy for recombinant expression of full-length monoclonal antibodies is to perform transient plasmid transfection of mammalian cells, resulting in sma
Autor:
Victor Greiff, Simon M Meng, Sai T. Reddy, Derek M Mason, William Kelton, Cristina Parola, Cédric R. Weber
Publikováno v:
Nucleic Acids Research, 46 (14)
Nucleic Acids Research
Nucleic Acids Research
Antibody engineering is performed to improve therapeutic properties by directed evolution, usually by high-throughput screening of phage or yeast display libraries. Engineering antibodies in mammalian cells offers advantages associated with expressio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a6f1ffb3cc86347a857950a738b4e89
Publikováno v:
Methods in Molecular Biology ISBN: 9781493987290
From the perspective of academic and small research laboratories, the most common and practical strategy for recombinant expression of full-length monoclonal antibodies is to perform transient plasmid transfection of mammalian cells, resulting in sma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b536e883b7455811beed00f08da960ea
https://doi.org/10.1007/978-1-4939-8730-6_7
https://doi.org/10.1007/978-1-4939-8730-6_7
Autor:
Cristina Parola, William Kelton, Sai T. Reddy, Kyle Ford, Mark Pogson, Theresa Pesch, Ann Cathrin Waindok
Publikováno v:
Scientific Reports
Scientific Reports, 7
Scientific Reports, 7
The development of programmable nucleases has enabled the application of new genome engineering strategies for cellular immunotherapy. While targeted nucleases have mostly been used to knock-out or knock-in genes in immune cells, the scarless exchang