Identification of Specific Inhibitors of Trypanosoma cruzi Malic Enzyme Isoforms by Target-Based HTS

Autor: Shane R. Wilkinson, Cristina Nowicki, Americo T. Ranzani, Artur T. Cordeiro

Publikováno v: SLAS Discovery. 22:1150-1161

Trypanosoma cruzi is the causative agent of Chagas disease. The lack of an efficient and safe treatment supports the research into novel metabolic targets, with the malic enzyme (ME) representing one such potential candidate. T. cruzi expresses a cyt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ede2363f40187b573214aa150926052
https://doi.org/10.1177/2472555217706649

Exploring biochemical and functional features of Leishmania major phosphoenolpyruvate carboxykinase

Autor: Cristina Nowicki, Máximo Hernán Sosa, Lucila Giordana

Publikováno v: Archives of Biochemistry and Biophysics. 583:120-129

This work reports the first functional characterization of leishmanial PEPCK. The recombinant Leishmania major enzyme (Lmj_PEPCK) exhibits equivalent kcat values for the phosphoenolpyruvate (PEP) and oxaloacetate (OAA) forming reactions. The apparent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd218ceb5e40a84c565081c62e4fc09b
https://doi.org/10.1016/j.abb.2015.07.015

Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes

Autor: Letícia Marchese, Cristina Nowicki, Brian S. Mantilla, Elizabeth M. F. Pral, Ariel Mariano Silber, Emilio F. Merino, Ludmila Nakamura Rapado, Maria B. Cassera, María Julia Barisón

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Journal of biological chemistry, 2017, Vol.292(21), pp.8964-8977 [Peer Reviewed Journal]

Trypanosoma cruzi, the etiological agent of Chagas disease, is a protozoan parasite with a complex life cycle involving a triatomine insect and mammals. Throughout its life cycle, the T. cruzi parasite faces several alternating events of cell divisio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6788f33e36a3ba179dcb435ceda21002
https://europepmc.org/articles/PMC5448128/

Cystathionine γ-lyase, an Enzyme Related to the Reverse Transsulfuration Pathway, is Functional in Leishmania spp

Autor: Marianela Santana, Ariel Mariano Silber, Cristina Nowicki, Lucila Giordana, Brian S. Mantilla

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

Leishmania parasites seem capable of producing cysteine by de novo biosynthesis, similarly to bacteria, some pathogenic protists, and plants. In Leishmania spp., cysteine synthase (CS) and cystathionine β-synthase (CBS) are expected to participate i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::862d56235125d4d28ec9a99357f0b8c2
https://doi.org/10.1111/jeu.12100

ANTIBODIES AGAINST HAPTENS IN THE STUDY OF STRUCTURE AND BIOLOGICAL ACTIVITY OF GROWTH HORMONE

Autor: Cristina Nowicki, José A. Santomé

Publikováno v: International Journal of Peptide and Protein Research. 13:379-384

The alpha-amino group of bovine Growth Hormone was selectively modified with TNBS with no detectable changes in growth promoting activity. TNP was used as hapten for the production of antibodies against the end terminal region of bGH. The region near

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ad242e37c31e2f4a27d738047b3464a
https://doi.org/10.1111/j.1399-3011.1979.tb01896.x