Výsledky vyhledávání - "Cristina Auriche"

Genomic Context Vectors and Artificial Chromosomes for Cystic Fibrosis Gene Therapy

Autor: Sante Di Gioia, Cristina Auriche, A. Christopher Boyd, Fiorentina Ascenzioni, Massimo Conese

Publikováno v: Current Gene Therapy. 7:175-187

Cystic fibrosis (CF) is caused by mutations of the CF transmembrane conductance regulator (CFTR) gene, which encodes a cAMP dependent chloride channel whose expression is finely tuned in space and time. Gene therapy approaches to CF lung disease have

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d6b2027e3d47afca2bb5c86752ff572
https://doi.org/10.2174/156652307780859026

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Functional human CFTR produced by a stable minichromosome

Autor: Cristina Auriche, Emanuela Caci, Daniela Carpani, Massimo Conese, Pierluigi Donini, Fiorentina Ascenzioni, Olga Zegarra-Moran

Publikováno v: EMBO reports. 3:862-868

Artificial chromosomes have been claimed to be the ideal vector for gene therapy, but their use has been hampered by an inability to produce stable and well designed molecules. We have used a structurally defined minichromosome to clone the human cys

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41311a17dd32ebf17bb05036aa2c666a
https://doi.org/10.1093/embo-reports/kvf174

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Use of a Human Minichromosome as a Cloning and Expression Vector for Mammalian Cells

Autor: Cristina Auriche, Cristiana Guiducci, Anna Maria Guerrini, Enza Piccolella, Pierluigi Donini, Fiorentina Ascenzioni

Publikováno v: Human Molecular Genetics. 8:1417-1424

A natural human minichromosome (MC1) derived from human chromosome 1 was shown to be linear and to have a size of 5.5 Mb. Human IL-2 cDNA and the neo gene were co-transfected into a MC1-containing human-CHO hybrid cell line. Integration of the foreig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f80472e53d14dfded756f46f316ea9e2
https://doi.org/10.1093/hmg/8.8.1417

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CFTR expression and activity from the human CFTR locus in BAC vectors, with regulatory regions, isolated by a single-step procedure

Autor: Stefano Castellani, Cristina Auriche, Silvia Pierandrei, Olga Zegarra-Moran, R. Melani, Massimo Conese, Marco Lucarelli, E G Di Domenico, Fiorentina Ascenzioni

Publikováno v: Università degli studi di Foggia-IRIS

We have assembled two BAC vectors containing a single fragment spanning the entire CFTR locus and including the upstream and downstream regions. The two vectors differ in size of the upstream region, and were recovered in Escherichia coli, with intac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dff51e7a311341a0a2e0a86b6481844
http://hdl.handle.net/11573/228572

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Burkholderia cenocepacia strains isolated from cystic fibrosis patients are apparently more invasive and more virulent than rhizosphere strains

Autor: Luisa, Pirone, Alessandra, Bragonzi, Alessio, Farcomeni, Moira, Paroni, Cristina, Auriche, Massimo, Conese, Luigi, Chiarini, Claudia, Dalmastri, Annamaria, Bevivino, Fiorentina, Ascenzioni

Publikováno v: Environmental microbiology. 10(10)

Given the widespread presence of Burkholderia cenocepacia in the rhizosphere it is important to determine whether rhizosphere strains are pathogenic for cystic fibrosis patients or not. Eighteen B. cenocepacia strains of rhizosphere and clinical orig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::04dce2c7b88074677c4a7b2114293f4f
https://pubmed.ncbi.nlm.nih.gov/18643926

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INFLUENCE OF PSEUDOMONAS AERUGINOSA ON THE GROWTH RATE AND INTERNALIZATION OF BURKHOLDERIA CENOCEPACIA

Autor: Cristina Auriche, Annamaria Bevivino, Claudia Dalmastri, Fiorentina Ascenzioni, S. Marinelli, Luisa Pirone

Publikováno v: Journal of Cystic Fibrosis. 7

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b56d5089795470b65a58a926a4d9f9c

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The Mec1p and Tel1p checkpoint kinases allow humanized yeast to tolerate chronic telomere dysfunctions by suppressing telomere fusions

Autor: Maria Pia Longhese, Enea Gino Di Domenico, Cristina Auriche, Fiorentina Ascenzioni, Valeria Viscardi, Eric Gilson

Publikováno v: DNA repair. 8(2)

In this work we report that budding yeasts carrying human-type telomeric repeats at their chromosome termini show a chronic activation of the Rad53-dependent DNA damage checkpoint pathway and a G2/M cell cycle delay. Furthermore, in the absence of ei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab9911769c394dca6200d7fb5e552f22
https://pubmed.ncbi.nlm.nih.gov/19007917

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Gene therapy progress and prospects: episomally maintained self-replicating systems

Autor: Cristina Auriche, Fiorentina Ascenzioni, Massimo Conese

Publikováno v: Gene therapy. 11(24)

The use of nonviral gene therapy vectors has been hampered by low level of transfection efficiency and lack of sustained gene expression. Episomal self-replicating systems may overcome these hurdles through their large packaging capacity, stability a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62c36f75b2a4815b5da50cb6f759b75b
https://pubmed.ncbi.nlm.nih.gov/15385951

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