Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients.

Autor: Ramírez JC; Laboratory of Molecular Biology of Chagas Disease (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina., Cura CI; Laboratory of Molecular Biology of Chagas Disease (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina., da Cruz Moreira O; Laboratory of Molecular Biology of Endemic Diseases, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Brazil., Lages-Silva E; Laboratory of Discipline of Parasitology, Universidade Federal do Triângulo Mineiro, Uberaba, Brazil., Juiz N; Laboratory of Molecular Biology of Chagas Disease (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina., Velázquez E; National Institute of Parasitology 'Dr. Mario Fatala Chabén', Buenos Aires, Argentina., Ramírez JD; Center for Research in Tropical Microbiology and Parasitology, Universidad de los Andes, Bogota, Colombia., Alberti A; Institute of Experimental Pathology, CONICET-Universidad Nacional de Salta, Salta, Argentina., Pavia P; Laboratory of Molecular Parasitology, Pontificia Universidad Javeriana, Bogota, Colombia., Flores-Chávez MD; National Center for Microbiology, Instituto de Salud Carlos III, Madrid, Spain., Muñoz-Calderón A; Institute of Tropical Medicine, Universidad Central de Venezuela, Caracas, Venezuela., Pérez-Morales D; Biomedical Research Institute, Universidad Nacional Autónoma de México, Mexico DF, Mexico., Santalla J; Laboratory of Parasitology and Molecular Biology, Instituto Nacional de Laboratorios en Salud, La Paz, Bolivia., Marcos da Matta Guedes P; Department of Microbiology and Parasitology, Universidade Federal do Rio Grande do Norte, Natal, Brazil., Peneau J; Hospital and University Laboratory-CH Andrée Rosemon, Cayenne, French Guiana., Marcet P; Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia., Padilla C; National Center for Public Health, Instituto Nacional de Salud, Lima, Peru., Cruz-Robles D; Laboratory of Genomics, Instituto Nacional de Cardiología 'Ignacio Chávez', Mexico DF, Mexico., Valencia E; Laboratory for Research in Infectious Diseases, Universidad Peruana Cayetano Heredia, Lima, Peru., Crisante GE; Department of Biology, Universidad de los Andes, Merida, Venezuela., Greif G; Molecular Biology Unit, Instituto Pasteur de Montevideo, Montevideo, Uruguay., Zulantay I; Basic Clinical Parasitology Laboratory, Universidad de Chile, Santiago, Chile., Costales JA; Research Center for Infectious Diseases, Pontificia Universidad Católica de Ecuador, Quito, Ecuador., Alvarez-Martínez M; Microbiology Department, Hospital Clinic and Barcelona Centre for International Health Research (CRESIB), Barcelona, Spain., Martínez NE; State Laboratory of Public Health, Acapulco, Mexico., Villarroel R; Biomedical Department, Instituto de Salud Pública, Santiago, Chile., Villarroel S; Laboratory of Molecular Biology, Universidad Mayor de San Simón, Cochabamba, Bolivia., Sánchez Z; Research Institute for Health Sciences, Universidad Nacional de Asunción, Asuncion, Paraguay., Bisio M; Laboratory of Molecular Biology of Chagas Disease (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina., Parrado R; Laboratory of Molecular Biology, Universidad Mayor de San Simón, Cochabamba, Bolivia., Maria da Cunha Galvão L; Department of Microbiology and Parasitology, Universidade Federal do Rio Grande do Norte, Natal, Brazil., Jácome da Câmara AC; Department of Microbiology and Parasitology, Universidade Federal do Rio Grande do Norte, Natal, Brazil., Espinoza B; Biomedical Research Institute, Universidad Nacional Autónoma de México, Mexico DF, Mexico., Alarcón de Noya B; Institute of Tropical Medicine, Universidad Central de Venezuela, Caracas, Venezuela., Puerta C; Laboratory of Molecular Parasitology, Pontificia Universidad Javeriana, Bogota, Colombia., Riarte A; National Institute of Parasitology 'Dr. Mario Fatala Chabén', Buenos Aires, Argentina., Diosque P; Institute of Experimental Pathology, CONICET-Universidad Nacional de Salta, Salta, Argentina., Sosa-Estani S; National Institute of Parasitology 'Dr. Mario Fatala Chabén', Buenos Aires, Argentina., Guhl F; Center for Research in Tropical Microbiology and Parasitology, Universidad de los Andes, Bogota, Colombia., Ribeiro I; Drugs for Neglected Diseases Initiative (DNDi), Geneva, Switzerland., Aznar C; Hospital and University Laboratory-CH Andrée Rosemon, Cayenne, French Guiana., Britto C; Laboratory of Molecular Biology of Endemic Diseases, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Brazil., Yadón ZE; Communicable Diseases and Health Analysis Department, Pan American Health Organization/World Health Organization (PAHO/WHO), Rio de Janeiro, Brazil., Schijman AG; Laboratory of Molecular Biology of Chagas Disease (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina. Electronic address: schijman@dna.uba.ar.

Publikováno v: The Journal of molecular diagnostics : JMD [J Mol Diagn] 2015 Sep; Vol. 17 (5), pp. 605-15.