Zobrazeno 1 - 10
of 4 791
pro vyhledávání: '"Cre-Lox recombination"'
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 6, Pp 2403-2419 (2021)
Summary The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are guided by transposon mutagenesis studie
Externí odkaz:
https://doaj.org/article/37be770e951f4fe5b809c00854c92a1a
Autor:
Shaw, Daniel1 (AUTHOR), Miravet‐Verde, Samuel1 (AUTHOR), Piñero‐Lambea, Carlos1 (AUTHOR), Serrano, Luis1,2,3 (AUTHOR) luis.serrano@crg.eu, Lluch‐Senar, Maria1,4 (AUTHOR) maria.lluch@pulmobio.com
Publikováno v:
Microbial Biotechnology. Nov2021, Vol. 14 Issue 6, p2403-2419. 17p.
Autor:
Behnam Enghiad, Chunshuai Huang, Fang Guo, Guangde Jiang, Bin Wang, S. Kasra Tabatabaei, Teresa A. Martin, Huimin Zhao
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonst
Externí odkaz:
https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b
Akademický článek
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Autor:
Teresa A. Martin, Behnam Enghiad, Guangde Jiang, Fang Guo, S Kasra Tabatabaei, Huimin Zhao, Bin Wang, Chunshuai Huang
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Nature Communications
Nature Communications
Direct cloning represents the most efficient strategy to access the vast number of uncharacterized natural product biosynthetic gene clusters (BGCs) for the discovery of novel bioactive compounds. However, due to their large size, repetitive nature,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b08bdc38abd8d6a78c5bdde8e77010e
https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b
https://doaj.org/article/42e3dde80df44f6a95cfb8762a0ff62b
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Publikováno v:
Microbial Biotechnology, 14 (6)
Microbial Biotechnology, Vol 14, Iss 6, Pp 2403-2419 (2021)
Microbial Biotechnology
Microbial Biotechnology, Vol 14, Iss 6, Pp 2403-2419 (2021)
Microbial Biotechnology
The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge of which genomic regions are dispensable. Typically, these efforts are guided by transposon mutagenesis studies, coupl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08d85b42c3d328de26ce7f019a2890fa
https://hdl.handle.net/20.500.11850/605644
https://hdl.handle.net/20.500.11850/605644
Publikováno v:
The FASEB Journal. 35
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2d5f8c0f90206562e56f7074fdb55657
https://doi.org/10.1096/fasebj.2021.35.s1.01974
https://doi.org/10.1096/fasebj.2021.35.s1.01974
Publikováno v:
Methods in Molecular Biology ISBN: 9781071607466
Intestinal stem cells are responsible for tissue renewal. The study of stem cell properties has become a major challenge in the field. We describe here a method based on Cre recombinase inducible lentivirus vectors that permits delivery of transgenes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6c3b1cbc8ef2f4dd7b5f4e54dc86206f
https://doi.org/10.1007/978-1-0716-0747-3_16
https://doi.org/10.1007/978-1-0716-0747-3_16
Autor:
Ravneet Kaur
Publikováno v:
Genomic, Proteomics, and Biotechnology ISBN: 9781003220831
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e8549c1ba9b27e79d3dfa8b6baa3a57d
https://doi.org/10.1201/9781003220831-5
https://doi.org/10.1201/9781003220831-5