An integrated protocol for targeted mutagenesis with CRISPR-Cas9 system in the pea aphid

Autor: Florence Gleonnec, Craig G. Webster, G. Le Trionnaire, Maryline Uzest, Gautier Richard, D. Tagu, Amaury Herpin, Baptiste Monsion, Bastien Cayrol, Sylvie Hudaverdian, E. Pichon, Maëlle Deshoux, Sylvie Tanguy

Publikováno v: Insect Biochemistry and Molecular Biology
Insect Biochemistry and Molecular Biology, Elsevier, 2019, 110, pp.34-44. ⟨10.1016/j.ibmb.2019.04.016⟩
Insect Biochemistry and Molecular Biology, 2019, 110, pp.34-44. ⟨10.1016/j.ibmb.2019.04.016⟩
Insect Biochemistry and Molecular Biology (110), 34-44. (2019)

International audience; CRISPR-Cas9 technology is a very efficient functional analysis tool and has been developed in several insects to edit their genome through injection of eggs with guideRNAs targeting coding sequences of genes of interest. Howev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78883322ea87ba800db7380a3117345e
https://doi.org/10.1016/j.ibmb.2019.04.016

Cuticular Structure Proteomics in the Pea Aphid

Autor: Maëlle, Deshoux, Victor, Masson, Karim, Arafah, Sébastien, Voisin, Natalia, Guschinskaya, Manuella, van Munster, Bastien, Cayrol, Craig G, Webster, Yvan, Rahbé, Stéphane, Blanc, Philippe, Bulet, Marilyne, Uzest

Publikováno v: Journal of Proteome Research

Aphids are phloem-feeding insects known as major pests in agriculture that are able to transmit hundreds of plant viruses. The majority of these viruses, classified as noncirculative, are retained and transported on the inner surface of the cuticle o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::76317bbab6b983fdc0595756c8de7cee
https://pubmed.ncbi.nlm.nih.gov/31991085

Development and Evaluation of ELISA and qRT-PCR for Identification of Squash vein yellowing virus in Cucurbits

Autor: William W. Turechek, Craig G. Webster, Scott Adkins, Chandrasekar S. Kousik, Weimin Li

Publikováno v: Plant Disease. 101:178-185

Squash vein yellowing virus (SqVYV) causes viral watermelon vine decline. To facilitate detection of SqVYV, enzyme linked-immunosorbent assay (ELISA) and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) diagnostic methods were d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c51f396aac5e8562d1b53e47ced1d235
https://doi.org/10.1094/pdis-06-16-0872-re

Widespread Occurrence and Low Genetic Diversity of Colombian datura virus in Brugmansia Suggest an Anthropogenic Role in Virus Selection and Spread

Autor: Carlye A. Baker, Craig G. Webster, Dan O. Chellemi, Mani Annamalai, Diann Achor, Scott Adkins

Publikováno v: Plant disease. 95(6)

Chellemi, D. O., Webster, C. G., Baker, C. A., Annamalai, M., Achor, D., and Adkins, S. 2011. Widespread occurrence and low genetic diversity of Colombian datura virus in Brugmansia suggest an anthropogenic role in virus selection and spread. Plant D

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a523ffb991ee1a3de3f0a6a8befbf4ac
https://pubmed.ncbi.nlm.nih.gov/30731909

Physiological Effects of Squash vein yellowing virus Infection on Watermelon

Autor: Carlye A. Baker, William W. Turechek, T. Greg McCollum, Susan E. Webb, Scott Adkins, Joseph P. Albano, Craig G. Webster, Pamela D. Roberts, Chandrasekar S. Kousik

Publikováno v: Plant disease. 97(9)

Squash vein yellowing virus (SqVYV) is the cause of viral watermelon vine decline. The virus is whitefly-transmitted, induces a systemic wilt of watermelon plants, and causes necrosis and discoloration of the fruit rind. In the field, SqVYV is often

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa8f8395c00437f0f7d38a7af5ff3cf4
https://pubmed.ncbi.nlm.nih.gov/30722421