AXL-Receptor Targeted 14FN3 Based Single Domain Proteins (Pronectins™) from 3 Synthetic Human Libraries as Components for Exploring Novel Bispecific Constructs against Solid Tumors

Autor: Craig A. Hokanson, Emanuela Zacco, Guido Cappuccilli, Tatjana Odineca, Roberto Crea

Publikováno v: Biomedicines, Vol 10, Iss 12, p 3184 (2022)

A highly specific AXL-receptor targeted family of non-immunoglobulin, single domain protein binders (Pronectins™) have been isolated from three (3) synthetic libraries that employ the human scaffold of the 14th domain of Fibronectin III (14FN3) and

Externí odkaz: https://doaj.org/article/f9beccc674ee4ecda081e0285a352de3

The First-In-Class Anti-AXL×CD3ε Pronectin™-Based Bispecific T-Cell Engager Is Active in Preclinical Models of Human Soft Tissue and Bone Sarcomas

Autor: Nicoletta Polerà, Antonia Mancuso, Caterina Riillo, Daniele Caracciolo, Stefania Signorelli, Katia Grillone, Serena Ascrizzi, Craig A. Hokanson, Francesco Conforti, Nicoletta Staropoli, Luigia Gervasi, Maria Teresa Di Martino, Mariamena Arbitrio, Giuseppe Nisticò, Roberto Crea, Pierosandro Tagliaferri, Giada Juli, Pierfrancesco Tassone

Publikováno v: Cancers. 15:1647

Sarcomas are heterogeneous malignancies with limited therapeutic options and a poor prognosis. We developed an innovative immunotherapeutic agent, a first-in-class Pronectin™-based Bispecific T-Cell Engager (pAXL×CD3ε), for the targeting of AXL,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e570facbe524e0b22382fcca7e68205c
https://doi.org/10.3390/cancers15061647

Hybrid Yeast–Bacteria Cloning System Used to Capture and Modify Adenoviral and Nonviral Genomes

Autor: Brian A. Donahue, Michael Mendez, Emery G. Dora, Craig A. Hokanson, Mitchell H. Finer, Marianne Rivkin

Publikováno v: Human Gene Therapy. 14:329-339

Adenoviral vectors are widely used to express transgenes in vitro and in vivo. A major obstacle to the generation of adenoviral vectors is the manipulation of the large (35 kb) adenoviral genome. We developed a hybrid yeast-bacteria cloning system fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f9b6644157631f22ac04f6777006f27
https://doi.org/10.1089/104303403321208934

Engineering highly thermostable xylanase variants using an enhanced combinatorial library method

Autor: Roberto Crea, Craig A. Behnke, William J. Coleman, Guido Cappuccilli, Craig A. Hokanson, Michael Mendez, Tatjana Odineca, Marino Bozic

Publikováno v: Protein engineering, designselection : PEDS. 24(8)

A new directed evolution method was used to enhance the thermostability of the wild-type GH11 xylanase 2 (known as BD-11) from Hypocrea jecorina (Trichoderma reesei). Both Look-Through Mutagenesis (LTM™), which is a method for rapidly screening sel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55a2cb9ec88e2d68946a843d9bd1f1e1
https://pubmed.ncbi.nlm.nih.gov/21708791

The First-In-Class Anti-AXL×CD3ε Pronectin™-Based Bispecific T-Cell Engager Is Active in Preclinical Models of Human Soft Tissue and Bone Sarcomas.

Autor: Polerà, Nicoletta¹ (AUTHOR), Mancuso, Antonia¹ (AUTHOR), Riillo, Caterina¹ (AUTHOR), Caracciolo, Daniele¹ (AUTHOR), Signorelli, Stefania¹ (AUTHOR), Grillone, Katia¹ (AUTHOR), Ascrizzi, Serena¹ (AUTHOR), Hokanson, Craig A.² (AUTHOR), Conforti, Francesco³ (AUTHOR), Staropoli, Nicoletta¹ (AUTHOR), Gervasi, Luigia¹ (AUTHOR), Di Martino, Maria Teresa¹ (AUTHOR), Arbitrio, Mariamena⁴ (AUTHOR), Nisticò, Giuseppe⁵ (AUTHOR), Crea, Roberto^2,5 (AUTHOR), Tagliaferri, Pierosandro¹ (AUTHOR), Juli, Giada¹ (AUTHOR) giadajuli@libero.it, Tassone, Pierfrancesco^1,6 (AUTHOR) giadajuli@libero.it

Publikováno v: Cancers. Mar2023, Vol. 15 Issue 6, p1647. 17p.