Structural Characterization of Black Widow Spider Dragline Silk Proteins CRP1 and CRP4

Autor: Mikayla Shanafelt, Taylor Rabara, Danielle MacArt, Caroline Williams, Ryan Hekman, Hyun Joo, Jerry Tsai, Craig Vierra

Publikováno v: Molecules, Vol 25, Iss 14, p 3212 (2020)

Spider dragline silk represents a biomaterial with outstanding mechanical properties, possessing high-tensile strength and toughness. In black widows at least eight different proteins have been identified as constituents of dragline silk. These repre

Externí odkaz: https://doaj.org/article/75cfa3bc69b34378831b088056f1841c

Role of BGS13 in the Secretory Mechanism of Pichia pastoris

Autor: Pachai Moua, Craig Vierra, Andreas H. Franz, Christopher A. Naranjo, Aaron Hang, Anita Jivan, Geoff P. Lin-Cereghino, Caroline Chou, Joyce J. Choi, Gina Myers, Christina Uribe, Ryan Hekman, Michelle Fong, Der Thor, Douglas D. Risser, Kai Her, Katherine H. de Sa Campos, Joan Lin-Cereghino, Maria N. Vo, Jared S Deyarmin, Taylor R. Rabara

Publikováno v: Applied and Environmental Microbiology. 85

The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular med

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2af2af66e5a9fdb378224fbba6b4be7f
https://doi.org/10.1128/aem.01615-19

Dragline Silk: A Fiber Assembled with Low-Molecular-Weight Cysteine-Rich Proteins

Autor: Thanh Pham, Taylor Crawford, Caroline Williams, Yang Hsia, Jerry Tsai, Tyler Chuang, Liang Zhao, Hyun Joo, Craig Vierra, Albert Lin

Publikováno v: Biomacromolecules. 15:4073-4081

Dragline silk has been proposed to contain two main protein constituents, MaSp1 and MaSp2. However, the mechanical properties of synthetic spider silks spun from recombinant MaSp1 and MaSp2 proteins have yet to approach natural fibers, implying the n

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bca64afbdca5e987c04b503dc1146dcc
https://doi.org/10.1021/bm5011239

Comprehensive Proteomic Analysis of Spider Dragline Silk from Black Widows: A Recipe to Build Synthetic Silk Fibers

Autor: Alisa Arata, Simmone Dyrness, Ryan Hekman, Craig Vierra, Taylor Crawford, Camille Larracas, Caroline Williams

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 17; Issue 9; Pages: 1537
International Journal of Molecular Sciences, Vol 17, Iss 9, p 1537 (2016)

The outstanding material properties of spider dragline silk fibers have been attributed to two spidroins, major ampullate spidroins 1 and 2 (MaSp1 and MaSp2). Although dragline silk fibers have been treated with different chemical solvents to elucida

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c58ab62ff922bffa7184b86fcaddf3b9
http://europepmc.org/articles/PMC5037812

Conserved C-Terminal Domain of Spider Tubuliform Spidroin 1 Contributes to Extensibility in Synthetic Fibers

Autor: Jeffery L. Yarger, Kimiko Agari, Simon Y. Tang, Warner S. Weber, Eric Gnesa, Yang Hsia, Geoff P. Lin-Cereghino, Joan Lin-Cereghino, Craig Vierra

Publikováno v: Biomacromolecules. 13:304-312

Spider silk is renowned for its extraordinary mechanical properties, having a balance of high tensile strength and extensibility. To date, the majority of studies have focused on the production of dragline silks from synthetic spider silk gene produc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8489973d7d8c04ba904a0e9285ed0401
https://doi.org/10.1021/bm201262n