Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells

Autor: Vivien Grant, Dáire Gannon, Eimear Lagan, Neza Alfazema, Craig Monger, Steven M. Pollard, Hannah K. Neikes, Orla Deevy, Darren J. Fitzpatrick, Raul Bardini Bressan, Evan Healy, Maria-Angeles Marqués-Torrejón, Adrian P. Bracken, Anthony M Doherty, Gerard L. Brien

Publikováno v: Brien, G L, Bressan, R B, Monger, C, Gannon, D, Lagan, E, Doherty, A M, Healy, E, Neikes, H, Fitzpatrick, D J, Deevy, O, Grant, V, Marqués-Torrejón, M-A, Alfazema, N, Pollard, S M & Bracken, A P 2021, ' Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells ', Nature Genetics, vol. 53, no. 8, pp. 1221-1232 . https://doi.org/10.1038/s41588-021-00897-w

Driver mutations in genes encoding histone H3 proteins resulting in p.Lys27Met substitutions (H3-K27M) are frequent in pediatric midline brain tumors. However, the precise mechanisms by which H3-K27M causes tumor initiation remain unclear. Here, we u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e035e9444657c8de908bac924ca715af
https://doi.org/10.1038/s41588-021-00897-w

Canonical PRC1 Recruitment Is Promoted by EZH1-PRC2 Independent of H3K27me3 in Quiescent Cells

Autor: Evan Healy, Rachel McCole, Craig Monger, Gerald L. Brien, Cheng Wang, Hannah K. Neikes, Frances Potjewyd, Michiel Vermeulen, Lindsey Ingerman James, Adrian Bracken

Publikováno v: SSRN Electronic Journal.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::820a2c8b149d078cb7bb017d54ba2a9a
https://doi.org/10.2139/ssrn.4089979

Sub physiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells

Autor: Niall Barron, Jonathan Bones, Paul S. Kelly, Ryan Hagan, Craig Monger, Justine Meiller, Patrick Floris, Krishna Motheramgari, Martin Clynes, Lin Zhang, Colin Clarke, Alan Costello, Clair Gallagher, Ioanna Tzani

RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes, however alternative mRNA splicing, has thus far, received little attention. In this study, we utilis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fcdb8e9e417bbf37b42b3c273917440
https://doi.org/10.1101/863175

A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data

Autor: Craig, Monger, Krishna, Motheramgari, John, McSharry, Niall, Barron, Colin, Clarke

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1603

In recent years, the publication of genome sequences for the Chinese hamster and Chinese hamster ovary (CHO) cell lines has facilitated study of these biopharmaceutical cell factories with unprecedented resolution. Our understanding of the CHO cell t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c0902b3368618fb37a2fc2c77dab6dc4
https://pubmed.ncbi.nlm.nih.gov/28493130

Towards next generation CHO cell biology: Bioinformatics methods for RNA-Seq-based expression profiling

Autor: Clair Gallagher, Paul S. Kelly, Colin Clarke, Craig Monger, Martin Clynes, Niall Barron

Publikováno v: Biotechnology journal. 10(7)

High throughput, cost effective next generation sequencing (NGS) has enabled the publication of genome sequences for Cricetulus griseus and several Chinese hamster ovary (CHO) cell lines. RNA-Seq, the utilization of NGS technology to study the transc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b443abe457855676d41ec990e0b8f107
https://pubmed.ncbi.nlm.nih.gov/26058739