Development of a Novel Label-Free Subunit HILIC-MS Method for Domain-Specific Free Thiol Identification and Quantitation in Therapeutic Monoclonal Antibodies.

Autor: Huang X; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Wang X; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Cotham VC; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Bramhall D; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Zhong J; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Zhao Y; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Qiu H; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Wang S; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States., Li N; Analytical Chemistry, Regeneron Pharmaceuticals Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707, United States.

Publikováno v: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2024 Oct 30. Date of Electronic Publication: 2024 Oct 30.

An Online Native Mass Spectrometry Approach for Fast, Sensitive, and Quantitative Assessment of Adeno-Associated Virus Capsid Content Ratios.

Autor: Cotham VC; Analytical Chemistry Group, Regeneron Pharmaceuticals Inc., Tarrytown, New York 10591, United States., Wang S; Analytical Chemistry Group, Regeneron Pharmaceuticals Inc., Tarrytown, New York 10591, United States., Li N; Analytical Chemistry Group, Regeneron Pharmaceuticals Inc., Tarrytown, New York 10591, United States.

Publikováno v: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2024 Jul 03; Vol. 35 (7), pp. 1567-1575. Date of Electronic Publication: 2024 Jun 18.

Clonally expanded B cells in multiple sclerosis bind EBV EBNA1 and GlialCAM.

Autor: Lanz TV; Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.; Department of Neurology, Mannheim Center for Translational Neurosciences (MCTN), Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.; Department of Neurology and National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany.; The Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, CA, USA., Brewer RC; Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.; The Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, CA, USA., Ho PP; Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Beckman Center for Molecular Medicine, Stanford, CA, USA., Moon JS; Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.; The Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, CA, USA., Jude KM; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Beckman Center for Molecular Medicine, Stanford, CA, USA., Fernandez D; Macromolecular Structure Knowledge Center, Stanford ChEM-H Institute, Stanford, CA, USA., Fernandes RA; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Beckman Center for Molecular Medicine, Stanford, CA, USA., Gomez AM; Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.; The Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, CA, USA., Nadj GS; Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.; The Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, CA, USA., Bartley CM; Weill Institute for Neurosciences, Department of Psychiatry and Behavioral Sciences, University of California San Francisco, San Francisco, CA, USA., Schubert RD; Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, San Francisco, CA, USA., Hawes IA; Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, San Francisco, CA, USA., Vazquez SE; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA., Iyer M; Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA., Zuchero JB; Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA., Teegen B; Institute of Experimental Immunology, Euroimmun AG, Lubeck, Germany., Dunn JE; Division of Neuroimmunology, Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA., Lock CB; Division of Neuroimmunology, Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA., Kipp LB; Division of Neuroimmunology, Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA., Cotham VC; Department of Biochemistry and Molecular Pharmacology, NYU Perlmutter Cancer Center, NYU School of Medicine, New York, NY, USA.; NYU Langone Health Proteomics Laboratory, Division of Advanced Research Technologies, NYU School of Medicine, New York, NY, USA., Ueberheide BM; Department of Biochemistry and Molecular Pharmacology, NYU Perlmutter Cancer Center, NYU School of Medicine, New York, NY, USA.; NYU Langone Health Proteomics Laboratory, Division of Advanced Research Technologies, NYU School of Medicine, New York, NY, USA., Aftab BT; Preclinical Science and Translational Medicine, Atara Biotherapeutics, South San Francisco, CA, USA., Anderson MS; Department of Medicine, Diabetes Center, University of California San Francisco, San Francisco, CA, USA., DeRisi JL; Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA, USA.; Chan Zuckerberg Biohub, University of California San Francisco, San Francisco, CA, USA., Wilson MR; Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, San Francisco, CA, USA., Bashford-Rogers RJM; Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK., Platten M; Department of Neurology, Mannheim Center for Translational Neurosciences (MCTN), Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.; Department of Neurology and National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany.; DKTK Clinical Cooperation Unit Neuroimmunology and Brain Tumor Immunology, German Cancer Research Center (DKFZ), Heidelberg, Germany., Garcia KC; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Beckman Center for Molecular Medicine, Stanford, CA, USA., Steinman L; Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Beckman Center for Molecular Medicine, Stanford, CA, USA., Robinson WH; Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. wrobins@stanford.edu.; The Geriatric Research, Education, and Clinical Center (GRECC), VA Palo Alto Health Care System, Palo Alto, CA, USA. wrobins@stanford.edu.

Publikováno v: Nature [Nature] 2022 Mar; Vol. 603 (7900), pp. 321-327. Date of Electronic Publication: 2022 Jan 24.

Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains.

Autor: Thyer R; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA., Shroff R; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA., Klein DR; Department of Chemistry, University of Texas at Austin, Austin, Texas, USA., d'Oelsnitz S; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA., Cotham VC; Department of Chemistry, University of Texas at Austin, Austin, Texas, USA., Byrom M; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA., Brodbelt JS; Department of Chemistry, University of Texas at Austin, Austin, Texas, USA., Ellington AD; Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, Texas, USA.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2018 Aug; Vol. 36 (7), pp. 624-631. Date of Electronic Publication: 2018 Jun 04.

Middle-Down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis.

Autor: Cotham VC; Department of Chemistry, ‡Center for Systems and Synthetic Biology, §Department of Biomedical Engineering, ∥Department of Chemical Engineering, ⊥Institute for Cellular and Molecular Biology, #Department of Molecular Biosciences, The University of Texas at Austin , Austin, Texas 78712, United States., Horton AP; Department of Chemistry, ‡Center for Systems and Synthetic Biology, §Department of Biomedical Engineering, ∥Department of Chemical Engineering, ⊥Institute for Cellular and Molecular Biology, #Department of Molecular Biosciences, The University of Texas at Austin , Austin, Texas 78712, United States., Lee J; Department of Chemistry, ‡Center for Systems and Synthetic Biology, §Department of Biomedical Engineering, ∥Department of Chemical Engineering, ⊥Institute for Cellular and Molecular Biology, #Department of Molecular Biosciences, The University of Texas at Austin , Austin, Texas 78712, United States., Georgiou G; Department of Chemistry, ‡Center for Systems and Synthetic Biology, §Department of Biomedical Engineering, ∥Department of Chemical Engineering, ⊥Institute for Cellular and Molecular Biology, #Department of Molecular Biosciences, The University of Texas at Austin , Austin, Texas 78712, United States., Brodbelt JS; Department of Chemistry, ‡Center for Systems and Synthetic Biology, §Department of Biomedical Engineering, ∥Department of Chemical Engineering, ⊥Institute for Cellular and Molecular Biology, #Department of Molecular Biosciences, The University of Texas at Austin , Austin, Texas 78712, United States.

Publikováno v: Analytical chemistry [Anal Chem] 2017 Jun 20; Vol. 89 (12), pp. 6498-6504. Date of Electronic Publication: 2017 May 26.