Výsledky vyhledávání - "Constance A. Sobsey"

Akademický článek

Measurement of CYP1A2 and CYP3A4 activity by a simplified Geneva cocktail approach in a cohort of free-living individuals: a pilot study

Autor: Constance A. Sobsey, Noor Mady, Vincent R. Richard, Andre LeBlanc, Thomas Zakharov, Christoph H. Borchers, R. Thomas Jagoe

Publikováno v: Frontiers in Pharmacology, Vol 15 (2024)

Introduction: The cytochrome P450 enzyme subfamilies, including CYP3A4 and CYP1A2, have a major role in metabolism of a range of drugs including several anti-cancer treatments. Many factors including environmental exposures, diet, diseaserelated syst

Externí odkaz: https://doaj.org/article/d4fe7fa4049f4675a002f17e3f4a7be1

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Immuno-MALDI-MS for Accurate Quantitation of Targeted Peptides from Volume-Restricted Samples

Autor: Constance A, Sobsey, Bjoern, Froehlich, Gerald, Batist, Christoph H, Borchers

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2515

The immuno-MALDI-MS method can be used to quantify low-abundance proteins from clinical samples that offer only a limited amount of material for analysis. An internal standard, in the form of a stable isotope-labeled peptide, is used to ensure reprod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::97102e052554c26f59ffa9a94b2be868
https://pubmed.ncbi.nlm.nih.gov/35776354

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Abstract P4-10-20: Protein quantitation assays for AKT and PTEN to better understand sensitivity and resistance of breast cancer patients to treatment with AKT inhibitor capivasertib

Autor: Constance A. Sobsey, Gerald Batist, René P. Zahedi, Sahar Ibrahim, Robert Popp, Christoph H. Borchers

Publikováno v: Cancer Research. 80:P4-10

Context: The PI3K/AKT/PTEN pathway is frequently aberrantly activated in breast cancer (BC) and involved in resistance to hormonal therapy. Multiple drugs targeting this pathway are approved or in development, including the pan-AKT inhibitor capivase

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e20807ff18b7fb86abc79a9d81e4ae36
https://doi.org/10.1158/1538-7445.sabcs19-p4-10-20

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A multiplexed, automated immuno-matrix assisted laser desorption/ionization mass spectrometry assay for simultaneous and precise quantitation of PTEN and p110 alpha in cell lines and tumor tissues

Autor: Oliver Pötz, Gerald Batist, René P. Zahedi, Marguerite Buchanan, Constance A. Sobsey, André LeBlanc, Robert Popp, Bjoern C. Froehlich, Mark Basik, Yassene Mohammed, Christoph H. Borchers, Adriana Aguilar-Mahecha, Alan Spatz, Sahar Ibrahim, Michael X. Chen

Publikováno v: Analyst, 146(21), 6566-6575. ROYAL SOC CHEMISTRY

The PI3-kinase/AKT/mTOR pathway plays a central role in cancer signaling. While p110 alpha is the catalytic alpha-subunit of PI3-kinase and a major drug target, PTEN is the main negative regulator of the PI3-kinase/AKT/mTOR pathway. PTEN is often dow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0abb6fd2f23e4bc02f4a42a6ad29be31
https://hdl.handle.net/1887/3250583

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Direct and Precise Measurement of Bevacizumab Levels in Human Plasma Based on Controlled Methionine Oxidation and Multiple Reaction Monitoring

Autor: Constance A. Sobsey, René P. Zahedi, Vincent R. Richard, Vanessa P. Gaspar, Alan Spatz, Christoph H. Borchers, Sahar Ibrahim

Publikováno v: ACS Pharmacol Transl Sci
ACS Pharmacology & Translational Science

[Image: see text] Bevacizumab is a monoclonal antibody which targets vascular endothelial growth factor A (VEGF-A) and is used to treat various cancers and recently COVID-19. The dosage recommendations for bevacizumab are determined on the basis of b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d521d820179eed64332c893f4812e86
https://pubmed.ncbi.nlm.nih.gov/33344903

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Systematic optimization of the iMALDI workflow for the robust and straightforward quantification of signaling proteins in cancer cells

Autor: André LeBlanc, Gerald Batist, Michael X. Chen, Constance A. Sobsey, Mark Basik, Alan Spatz, René P. Zahedi, Robert Popp, Christoph H. Borchers, Oliver Poetz, Adriana Aguilar-Mahecha, Bjoern C. Froehlich, Sahar Ibrahim, Yassene Mohammed

Publikováno v: Proteomics. Clinical Applications
Proteomics: Clinical Applications, 14(5). WILEY-V C H VERLAG GMBH

Purpose Immuno-MALDI (iMALDI) combines immuno-enrichment of biomarkers with MALDI-MS for fast, precise, and specific quantitation, making it a valuable tool for developing clinical assays. iMALDI assays are optimized for the PI3-kinase signaling path

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e21b141cb7fd9d5eebf6967d37cf9ea1
https://doi.org/10.1002/prca.202000034

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Development and evaluation of a liquid chromatography–mass spectrometry method for rapid, accurate quantitation of malondialdehyde in human plasma

Autor: Karen Lin, Anthony J. Levitt, Jun Han, Christoph H. Borchers, Walter Swardfager, Constance A. Sobsey

Publikováno v: Journal of Chromatography B. :205-212

Malondialdhyde (MDA) is a commonly used marker of lipid peroxidation in oxidative stress. To provide a sensitive analytical method that is compatible with high throughput, we developed a multiple reaction monitoring-mass spectrometry (MRM-MS) approac

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::810cdc755d1c5d5bba3fdbcf969f1dcf
https://doi.org/10.1016/j.jchromb.2016.07.013

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Abstract B21: Protein quantitation assays for Akt, PI3K p110α, and PTEN to assess PI3K pathway activity in tumor tissue

Autor: Mark Basik, Bjoern C. Froehlich, Sahar Ibrahim, Gerald Batist, Robert Popp, Adriana Aguilar-Mahecha, Christoph H. Borchers, Constance A. Sobsey

Publikováno v: Molecular Cancer Research. 18:B21-B21

Introduction: Many clinical trials for inhibitors targeting the PI3K pathway now include genetic screening of tumors to try to select probable treatment responders. Genetic testing identifies some relevant mutations, but this approach does not addres

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b0ae9e84944bbf8f6d0f5b23d035b509
https://doi.org/10.1158/1557-3125.pi3k-mtor18-b21

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