Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Conrad A. Vink"'
Autor:
Helin Sertkaya, Laura Hidalgo, Mattia Ficarelli, Dorota Kmiec, Adrian W. Signell, Sadfer Ali, Hannah Parker, Harry Wilson, Stuart J.D. Neil, Michael H. Malim, Conrad A. Vink, Chad M. Swanson
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss , Pp 147-157 (2021)
The antiviral protein ZAP binds CpG dinucleotides in viral RNA to inhibit replication. This has likely led to the CpG suppression observed in many RNA viruses, including retroviruses. Sequences added to retroviral vector genomes, such as internal pro
Externí odkaz:
https://doaj.org/article/f3e41db9b0604b7a84a4efc502459f51
Autor:
Nathan P. Sweeney, Conrad A. Vink
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss , Pp 574-584 (2021)
Lentiviral vectors are showing success in the clinic, but producing enough vector to meet the growing demand is a major challenge. Furthermore, next-generation gene therapy vectors encode multiple genes resulting in larger genome sizes, which is repo
Externí odkaz:
https://doaj.org/article/0d3209f99aca4bab821a70721ae1337a
Autor:
Helin Sertkaya, Mattia Ficarelli, Nathan P. Sweeney, Hannah Parker, Conrad A. Vink, Chad M. Swanson
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Abstract Many lentiviral vectors used for gene therapy are derived from HIV-1. An optimal vector genome would include only the viral sequences required for transduction efficiency and gene expression to minimize the amount of foreign sequence inserte
Externí odkaz:
https://doaj.org/article/9e4cd1425eb842c698f1446a5655b4b4
Autor:
Yu Hua Chen, Celeste Pallant, Christopher J. Sampson, Alessia Boiti, Sabine Johnson, Pijus Brazauskas, Philip Hardwicke, Michela Marongiu, Vanesa M. Marinova, Marlene Carmo, Nathan P. Sweeney, Ashkenaz Richard, Anthony Shillings, Peter Archibald, Eva Puschmann, Bernadette Mouzon, David Grose, Miriam Mendez-Tavio, Mao Xiang Chen, Stephen R.C. Warr, Tarik Senussi, Paul S. Carter, Sean Baker, Cindy Jung, Martijn H. Brugman, Steven J. Howe, Conrad A. Vink
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss , Pp 47-57 (2020)
Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient transf
Externí odkaz:
https://doaj.org/article/ac91c5a727084eccae608b8576a4179f
Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss , Pp 307-319 (2020)
Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging prope
Externí odkaz:
https://doaj.org/article/361803ffb87746f49f87ccee3b8b8885
Autor:
John R. Counsell, Zeinab Asgarian, Jinhong Meng, Veronica Ferrer, Conrad A. Vink, Steven J. Howe, Simon N. Waddington, Adrian J. Thrasher, Francesco Muntoni, Jennifer E. Morgan, Olivier Danos
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Abstract Duchenne Muscular Dystrophy (DMD) is caused by a lack of dystrophin expression in patient muscle fibres. Current DMD gene therapy strategies rely on the expression of internally deleted forms of dystrophin, missing important functional domai
Externí odkaz:
https://doaj.org/article/a4e4bc5af0674a76abc106a806bbacbb
Publikováno v:
Nucleic Acids Research
Clinical usage of lentiviral vectors is now established and increasing but remains constrained by vector titer with RNA packaging being a limiting factor. Lentiviral vector RNA is packaged through specific recognition of the packaging signal on the R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d3fe067d2cc2cf29277d01d0edd9a1c
https://doi.org/10.1093/nar/gkab1206
https://doi.org/10.1093/nar/gkab1206
Autor:
Hannah Parker, Sadfer Ali, Chad M. Swanson, Dorota Kmiec, Conrad A. Vink, Michael H. Malim, Laura Hidalgo, Harry Wilson, Mattia Ficarelli, Stuart J. D. Neil, Adrian W. Signell, Helin Sertkaya
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 23, Iss, Pp 147-157 (2021)
Molecular Therapy. Methods & Clinical Development
Molecular Therapy. Methods & Clinical Development
The antiviral protein ZAP binds CpG dinucleotides in viral RNA to inhibit replication. This has likely led to the CpG suppression observed in many RNA viruses, including retroviruses. Sequences added to retroviral vector genomes, such as internal pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::371e288c4ea5f5d7a8619a9f4d672c34
http://www.sciencedirect.com/science/article/pii/S2329050121001340
http://www.sciencedirect.com/science/article/pii/S2329050121001340
Autor:
P. Hardwicke, Christopher J. Sampson, Martijn H. Brugman, Cindy Jung, Yu Hua Chen, Bernadette Mouzon, Eva Puschmann, Conrad A. Vink, Stephen R.C. Warr, Paul S. Carter, Ashkenaz Richard, Steven J. Howe, Alessia Boiti, Peter Archibald, Vanesa M. Marinova, Michela Marongiu, Sean Baker, Mao Xiang Chen, Sabine Johnson, Tarik Senussi, Miriam Mendez-Tavio, Nathan P. Sweeney, Celeste Pallant, Marlene Carmo, David Grose, Pijus Brazauskas, Anthony Shillings
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss, Pp 47-57 (2020)
Molecular Therapy. Methods & Clinical Development
Molecular Therapy. Methods & Clinical Development
Stable suspension producer cell lines for the production of vesicular stomatitis virus envelope glycoprotein (VSVg)-pseudotyped lentiviral vectors represent an attractive alternative to current widely used production methods based on transient transf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e6603ce57bb9f5dfdfffc4d0f96421e
http://www.sciencedirect.com/science/article/pii/S2329050120301777
http://www.sciencedirect.com/science/article/pii/S2329050120301777
Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss, Pp 307-319 (2020)
Molecular Therapy. Methods & Clinical Development
Molecular Therapy. Methods & Clinical Development
Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging prope
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99c8060d947df235329de9845d471543
https://doi.org/10.1016/j.omtm.2020.09.010
https://doi.org/10.1016/j.omtm.2020.09.010