Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Colin D. Whitmore"'
Autor:
Lynn A. Gennaro, Colin D. Whitmore
Publikováno v:
ELECTROPHORESIS. 33:1550-1556
Tryptic peptide mapping is routinely used in the biotech industry to confirm primary sequence, cell line stability, and to analyze posttranslational modifications. Peptide analysis is generally done by reverse phase liquid chromatography with UV or m
Autor:
Ole Hindsgaul, Norman J. Dovichi, John U. White, Monica M. Palcic, David Essaka, Pradip Rathod, Colin D. Whitmore
Publikováno v:
Analytical Chemistry. 82:9955-9958
The metabolism of glycosphingolipids by the malaria-causing parasite Plasmodium falciparum plays an important role in the progression of the disease. We report a new and highly sensitive method to monitor the uptake of glycosphingolipids in infected
Publikováno v:
Talanta. 80:744-748
An ultrasensitive laser-induced fluorescence detector was used with capillary electrophoresis for the study of 5-carboxy-tetramethylrhodamine. The raw signal from the detector provided roughly three orders of magnitude dynamic range. The signal satur
Publikováno v:
ELECTROPHORESIS. 30:4071-4074
An ink jet printer valve and nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate. The system was evaluated by separation of a set of standard peptides. That separation generated up to 40,000 theoretical
Autor:
Norman J. Dovichi, Colin D. Whitmore, Mary E. Lidstrom, Sarah C. McQuaide, Cuiru Zhu, Anna K. Boardman
Publikováno v:
Analytical Chemistry. 80:7631-7634
We report a system that allows the simultaneous aspiration of one or more cells into each of five capillaries for electrophoresis analysis. A glass wafer was etched to create an array of 1 nL wells. The glass was treated with poly(2-hydroxyethyl meth
Autor:
Daniella Cohen, Norman J. Dovichi, Jane A. Dickerson, Emily H. Turner, Ole Hindsgaul, Colin D. Whitmore, Monica M. Palcic
Publikováno v:
Annual Review of Analytical Chemistry. 1:165-190
Cytometry deals with the analysis of the composition of single cells. Flow and image cytometry employ antibody-based stains to characterize a handful of components in single cells. Chemical cytometry, in contrast, employs a suite of powerful analytic
Autor:
Colin D. Whitmore, Emily H. Turner, Daniella Cohen, Haley R. Pugsley, Norman J. Dovichi, Cuiru Zhu, David G. Gomez
Publikováno v:
Analytical and Bioanalytical Chemistry. 390:223-226
The long-term goal of systems biology is to predict the response of an organism to a genetic or environmental change. However, such predictions are extremely challenging for whole organisms, even for the simplest organism. Instead, it is common to co
Publikováno v:
Analytical Chemistry. 79:5139-5142
Glycosphingolipids are found on all vertebrate cells and constitute major cell surface determinants on all nerve cells, where they contribute to cellular diversity and function. We report a method for the analysis of glycosphingolipid metabolism in s
Autor:
Rita Martins, Norman J. Dovichi, Colin D. Whitmore, Monica M. Palcic, Guido Tettamanti, Ole Hindsgaul, E. Andreas Larsson, Ulf Olsson, Ronald L. Schnaar
Publikováno v:
Carbohydrate Research. 342:482-489
Ganglioside GM1 and its seven potential catabolic products: asialo-GM1, GM2, asialo-GM2, GM3, Lac-Cer, Glc-Cer and Cer, were labeled with tetramethylrhodamine (TMR) to permit ultra-sensitive analysis using laser-induced fluorescence (LIF) detection.
Autor:
David G. Gomez, Megan R. Jones, Wes W. C. Quigley, Michael Eggertson, James R. Kraly, Xinya He, Anna K. Boardman, Norman J. Dovichi, Cuiru Zhu, Colin D. Whitmore
Publikováno v:
Analytical Chemistry. 79:765-768
In two-dimensional capillary electrophoresis, a sample undergoes separation in the first dimension capillary by sieving electrophoresis. Fractions are periodically transferred across an interface into a second dimension capillary, where components ar