GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control

Autor: Hickey, Kelsey L., Dickson, Kimberley, Cogan, J. Zachery, Replogle, Joseph M., Schoof, Michael, D’Orazio, Karole N., Sinha, Niladri K., Hussmann, Jeffrey A., Jost, Marco, Frost, Adam, Green, Rachel, Weissman, Jonathan S., Kostova, Kamena K.

Publikováno v: In Molecular Cell 17 September 2020 79(6):950-962

Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing

Autor: Nuñez, James K, Chen, Jin, Pommier, Greg C, Cogan, J Zachery, Replogle, Joseph M, Adriaens, Carmen, Ramadoss, Gokul N, Shi, Quanming, Hung, King L, Samelson, Avi J, Pogson, Angela N, Kim, James YS, Chung, Amanda, Leonetti, Manuel D, Chang, Howard Y, Kampmann, Martin, Bernstein, Bradley E, Hovestadt, Volker, Gilbert, Luke A, Weissman, Jonathan S

Publikováno v: Cell, vol 184, iss 9

A general approach for heritably altering gene expression has the potential to enable many discovery and therapeutic efforts. Here, we present CRISPRoff-a programmable epigenetic memory writer consisting of a single dead Cas9 fusion protein that esta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::1f1808be79b997f959552b57b33b0093
https://escholarship.org/uc/item/4ph8w0v1

Pervasive functional translation of noncanonical human open reading frames

Autor: Chen, Jin, Brunner, Andreas-David, Cogan, J Zachery, Nuñez, James K, Fields, Alexander P, Adamson, Britt, Itzhak, Daniel N, Li, Jason Y, Mann, Matthias, Leonetti, Manuel D, Weissman, Jonathan S

Publikováno v: Science (New York, N.Y.), vol 367, iss 6482

Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based screening strategy to identify hundreds of noncanonical CDSs that are e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______325::22db0ec6da63a7b49c18bc82a679fcde
https://escholarship.org/uc/item/96m1n5z5

Pervasive functional translation of noncanonical human open reading frames.

Autor: Chen J; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA., Brunner AD; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried 82152, Germany., Cogan JZ; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA., Nuñez JK; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA., Fields AP; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA., Adamson B; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.; Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA., Itzhak DN; Cell Atlas Initiative, Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Li JY; Cell Atlas Initiative, Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Mann M; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried 82152, Germany.; Clinical Proteomics Group, Proteomics Program, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen 2200, Denmark., Leonetti MD; Cell Atlas Initiative, Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Weissman JS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA. jonathan.weissman@ucsf.edu.; Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2020 Mar 06; Vol. 367 (6482), pp. 1140-1146.