Corona Detective: a simple, scalable, and robust SARS-CoV-2 detection method based on reverse transcription loop-mediated isothermal amplification.

Autor: Aidelberg, Guy, Aronoff, Rachel, Eliseeva, Tatiana, Quero, Francisco Javier, Vielfaure, Hortense, Codyre, Martin, Hadasch, Kathrin, Lindner, Ariel B.

Publikováno v: Journal of Biomolecular Techniques; Sep2021, Vol. 32 Issue 3, p1-9, 9p

Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.

Autor: Moore KJM; School of Science and Engineering, Ateneo de Manila University, Quezon City 1108, Philippines., Cahill J; Metamer Labs, Boston, MA 02111-1929, USA., Aidelberg G; Université de Paris, INSERM U1284, Center for Research and Interdisciplinarity (CRI), 75006 Paris, France.; Just One Giant Lab, Centre de Recherches Interdisciplinaires (CRI), 75004 Paris, France., Aronoff R; Just One Giant Lab, Centre de Recherches Interdisciplinaires (CRI), 75004 Paris, France.; Action for Genomic Integrity Through Research! (AGiR!), Lausanne, Switzerland.; Association Hackuarium, Lausanne, Switzerland., Bektaş A; Oakland Genomics Center, Oakland, CA 94609, USA., Bezdan D; Institute of Medical Genetics and Applied Genomics, University of Tübingen, 72076 Tübingen, Germany.; NGS Competence Center Tübingen (NCCT), University of Tübingen, 72076 Tübingen, Germany.; Poppy Health, Inc, San Francisco, CA 94158, USA.; Institute of Medical Virology and Epidemiology of Viral Diseases, University Hospital, 72076 Tübingen, Germany., Butler DJ; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA., Chittur SV; Center for Functional Genomics, Department of Biomedical Sciences, School of Public Health, University at Albany, State University of New York, Rensselaer, 12222, USA., Codyre M; GiantLeap Biotechnology Ltd, Wicklow A63 Kv91, Ireland., Federici F; ANID, Millennium Science Initiative Program, Millennium Institute for Integrative Biology (iBio), Institute for Biological and Medical Engineering, Schools of Engineering, Biology and Medicine, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Tanner NA; New England Biolabs, Ipswich, MA 01938, USA., Tighe SW; University of Vermont, Burlington, 05405, USA., True R; FloodLAMP Biotechnologies, San Carlos, CA 94070, USA., Ware SB; Just One Giant Lab, Centre de Recherches Interdisciplinaires (CRI), 75004 Paris, France.; BioBlaze Community Bio Lab, 1800 W Hawthorne Ln, Ste J-1, West Chicago, IL 60185, USA.; Blossom Bio Lab, 1800 W Hawthorne Ln, Ste K-2, West Chicago, IL 60185, USA., Wyllie AL; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Afshin EE; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA.; The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY 10065, USA., Bendesky A; Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, NY 10027, USA.; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA., Chang CB; Department of Chemical and Biological Engineering, Montana State University, Bozeman, 59717, USA.; Center for Biofilm Engineering, Montana State University, Bozeman, 59717, USA., Dela Rosa R 2nd; School of Science and Engineering, Ateneo de Manila University, Quezon City 1108, Philippines., Elhaik E; Department of Biology, Lund University, Sölvegatan 35, Lund, Sweden., Erickson D; Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14850, USA., Goldsborough AS; RNAssist Ltd, Cambridge CB1 1EE, England., Grills G; Department of Microbiology, University of Pennsylvania, Philadelphia, 19104, USA., Hadasch K; Université de Paris, INSERM U1284, Center for Research and Interdisciplinarity (CRI), 75006 Paris, France.; Department of Biology, Membrane Biophysics, Technische Universität Darmstadt, 64289 Darmstadt, Germany.; Lab3 eV, Labspace Darmstadt, 64295 Darmstadt, Germany.; IANUS Verein für Friedensorientierte Technikgestaltung eV, 64289 Darmstadt, Germany., Hayden A; Center for Functional Genomics, Department of Biomedical Sciences, School of Public Health, University at Albany, State University of New York, Rensselaer, 12222, USA., Her SY; Metamer Labs, Boston, MA 02111-1929, USA., Karl JA; Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Madison 53705, USA., Kim CH; GoDx, Inc, Madison, WI 53719, USA., Kriegel AJ; Medical College of Wisconsin, Milwaukee, 53226, USA., Kunstman T; University of Colorado, Boulder, CO 80309, USA., Landau Z; Department of Computer Science, University of California, Berkeley, Berkeley, 94720, USA., Land K; Mologic, Centre for Advanced Rapid Diagnostics, (CARD), Bedford Technology Park, Thurleigh MK44 2YA, England.; Department of Electrical, Electronic and Computer Engineering, University of Pretoria, 0028 Pretoria, South Africa., Langhorst BW; New England Biolabs, Ipswich, MA 01938, USA., Lindner AB; Université de Paris, INSERM U1284, Center for Research and Interdisciplinarity (CRI), 75006 Paris, France., Mayer BE; Department of Biology, Membrane Biophysics, Technische Universität Darmstadt, 64289 Darmstadt, Germany.; Lab3 eV, Labspace Darmstadt, 64295 Darmstadt, Germany., McLaughlin LA; IC Mobile Lab Ltd, SpacePortx, M1 1DZ Manchester, England., McLaughlin MT; Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Madison 53705, USA., Molloy J; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge CB3 0AS, England., Mozsary C; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA., Nadler JL; Department of Pharmacology, New York Medical College, Valhalla, 10595, USA., D'Silva M; Department of Pharmacology, New York Medical College, Valhalla, 10595, USA., Ng D; Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA., O'Connor DH; Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Madison 53705, USA., Ongerth JE; University of Wollongong, Environmental Engineering, Wollongong NSW 2522, Australia., Osuolale O; Applied Environmental Metagenomics and Infectious Diseases Research (AEMIDR), Department of Biological Sciences, Elizade University, Ilara Mokin, Nigeria., Pinharanda A; Department of Biological Sciences, Columbia University, New York, NY 10027, USA., Plenker D; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA., Ranjan R; Genomics Resource Laboratory, Institute for Applied Life Sciences, University of Massachusetts, Amherst, 01003, USA., Rosbash M; Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, MA 02453, USA., Rotem A; Metamer Labs, Boston, MA 02111-1929, USA., Segarra J; Metamer Labs, Boston, MA 02111-1929, USA., Schürer S; University of Florida, Miami, 33146, USA., Sherrill-Mix S; Department of Microbiology, University of Pennsylvania, Philadelphia, 19104, USA., Solo-Gabriele H; University of Florida, Miami, 33146, USA., To S; School of Science and Engineering, Ateneo de Manila University, Quezon City 1108, Philippines., Vogt MC; Department of Biological Sciences, Columbia University, New York, NY 10027, USA., Yu AD; Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, MA 02453, USA., Mason CE; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10065, USA.; The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY 10065, USA.; The Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY 10065, USA.

Publikováno v: Journal of biomolecular techniques : JBT [J Biomol Tech] 2021 Sep; Vol. 32 (3), pp. 228-275.