Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

Autor: Nikolaj L. Hansen, Karel Miettinen, Yong Zhao, Codruta Ignea, Aggeliki Andreadelli, Morten H. Raadam, Antonios M. Makris, Birger L. Møller, Dan Stærk, Søren Bak, Sotirios C. Kampranis

Publikováno v: Microbial Cell Factories, Vol 19, Iss 1, Pp 1-17 (2020)

Abstract Background Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is l

Externí odkaz: https://doaj.org/article/4893ec1389c84ef0a83e0925cf991ed9

Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast

Autor: Codruta Ignea, Anastasia Athanasakoglou, Aggeliki Andreadelli, Maria Apostolaki, Minas Iakovides, Euripides G. Stephanou, Antonios M. Makris, Sotirios C. Kampranis

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)

Abstract Plants synthesize numerous specialized metabolites (also termed natural products) to mediate dynamic interactions with their surroundings. The complexity of plant specialized metabolism is the result of an inherent biosynthetic plasticity ro

Externí odkaz: https://doaj.org/article/b63b2a195d8b415fa01df1947fdecad9

Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate

Autor: Sotirios C. Kampranis, Codruta Ignea, Antonios M. Makris, Mohammed Saddik Motawia, Claudia E. Vickers, Morten H. Raadam

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
Ignea, C, Raadam, M H, Motawia, M S, Makris, A M, Vickers, C E & Kampranis, S C 2019, ' Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate ', Nature Communications, vol. 10 . https://doi.org/10.1038/s41467-019-11290-x
Nature Communications

Synthetic biology efforts for the production of valuable chemicals are frequently hindered by the structure and regulation of the native metabolic pathways of the chassis. This is particularly evident in the case of monoterpenoid production in Saccha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::767e7c929a742d546482f4a48e7e31c5
https://doaj.org/article/5de8de4ce8a04318b1738fc8284a2b44

Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol

Autor: Søren Bak, Yong Zhao, Sotirios C. Kampranis, Karel Miettinen, Antonios M. Makris, Aggeliki Andreadelli, Nikolaj Lervad Hansen, Dan Staerk, Morten H. Raadam, Codruta Ignea, Birger Lindberg Møller

Publikováno v: Microbial Cell Factories
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-17 (2020)
Hansen, N L, Miettinen, K, Zhao, Y, Ignea, C, Andreadeli, A, Raadam, M H, Makris, A M, Møller, B L, Stærk, D, Bak, S & Kampranis, S 2020, ' Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol ', Microbial Cell Factories, vol. 19, 15 . https://doi.org/10.1186/s12934-020-1284-9

Background Celastrol is a promising anti-obesity agent that acts as a sensitizer of the protein hormone leptin. Despite its potent activity, a sustainable source of celastrol and celastrol derivatives for further pharmacological studies is lacking. R

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff0c08dc94bbd9d27557e642fdc2fc03
http://europepmc.org/articles/PMC6988343

Synthesis of 11-carbon terpenoids in yeast using protein and metabolic engineering

Autor: Codruta, Ignea, Marianna, Pontini, Mohammed S, Motawia, Massimo E, Maffei, Antonios M, Makris, Sotirios C, Kampranis

Publikováno v: Nature chemical biology. 14(12)

One application of synthetic biology is the redesign of existing biological systems to acquire new functions. In this context, expanding the chemical code underlying key biosynthetic pathways will lead to the synthesis of compounds with new structure

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f21e2aba0a552e424ae0cefccb7897b4
https://pubmed.ncbi.nlm.nih.gov/30429603