Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions

Autor: Alice M. Banks, Colette J. Whitfield, Steven R. Brown, David A. Fulton, Sarah A. Goodchild, Christopher Grant, John Love, Dennis W. Lendrem, Jonathan E. Fieldsend, Thomas P. Howard

Publikováno v: Computational and Structural Biotechnology Journal, Vol 20, Iss, Pp 218-229 (2022)
Computational and Structural Biotechnology Journal

Graphical abstract
Highlights • Novel cell-free protein synthesis reaction buffer improves performance by 400%. • Enhanced performance is maintained across the synthesis of different proteins. • Protein synthesis performance is robust acro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75d8a1b131f6dc02f2654a6fd7aef7a7
http://www.sciencedirect.com/science/article/pii/S2001037021005213

Multiblock metabolomics: An approach to elucidate whole-body metabolism with multiblock principal component analysis

Autor: Katsuhiko Sasaki, Chihiro Hayashi, Ko Igami, Tomoko Katahira, Kazuhiro Tanabe

Publikováno v: Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 1956-1965 (2021)

Graphical abstract
Highlights • “Multiblock metabolomics” elucidates the global metabolic network in a whole body. • “Multiblock metabolomics” combines LC/MS-based metabolomics with multiblock PCA. • “Multiblock metabolomics” h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81add9a8173efa684398bfcb0e88d68b
http://europepmc.org/articles/PMC8086023

A GX2GX3G motif facilitates acyl chain sequestration by Saccharomyces cerevisiae acyl carrier protein

Autor: Usha Yadav, Garima, Monica Sundd, Rashima Prem

Publikováno v: The Journal of Biological Chemistry

Saccharomyces cerevisiae acyl carrier protein (ScACP) is a component of the large fungal fatty acid synthase I (FAS I) complex. ScACP comprises two subdomains: a conserved ACP domain that shares extensive structural homology with other ACPs, and a un

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1e86f6cfdb5db96f66d94f13f683914
http://europepmc.org/articles/PMC8683515

The Many Faces of Cobalamin (Vitamin B12) Deficiency

Autor: M. Rebecca Heiner-Fokkema, Hanneke J C M Wouters, Melanie M. van der Klauw, Bruce H. R. Wolffenbuttel

Publikováno v: Mayo Clinic Proceedings: Innovations, Quality & Outcomes, Vol 3, Iss 2, Pp 200-214 (2019)
Mayo Clinic Proceedings: Innovations, Quality & Outcomes

Although cobalamin (vitamin B12) deficiency was described over a century ago, it is still difficult to establish the correct diagnosis and prescribe the right treatment. Symptoms related to vitamin B12 deficiency may be diverse and vary from neurolog

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::594da74ece54865937731a5077b3840c
http://www.sciencedirect.com/science/article/pii/S2542454819300335

Metallo-β-lactamase domain-containing protein 2 is S-palmitoylated and exhibits acyl-CoA hydrolase activity

Autor: Martin Ian P. Malgapo, Maurine E. Linder, Jenelle M. Safadi

Publikováno v: The Journal of Biological Chemistry

Members of the metallo-β-lactamase (MBL) superfamily of enzymes harbor a highly conserved αββα MBL-fold domain and were first described as inactivators of common β-lactam antibiotics. In humans, these enzymes have been shown to exhibit diverse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35687f6b81fd6d337a77588dba5035d5
http://europepmc.org/articles/PMC7949124