High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

Autor: Masson, Aurore, Escande, Paul, Frongia, Céline, Clouvel, Grégory, Ducommun, Bernard, Lorenzo, Corinne

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2015, 5 (1), ⟨10.1038/srep16898⟩

Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the

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https://hal.archives-ouvertes.fr/hal-02996834

High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM.

Autor: Masson A; Université de Toulouse, ITAV-USR3505, F-31106 Toulouse, France.; CNRS, ITAV-USR3505, F-31106 Toulouse, France., Escande P; Université de Toulouse, ITAV-USR3505, F-31106 Toulouse, France.; CNRS, ITAV-USR3505, F-31106 Toulouse, France.; ISAE, DISC, F-31106 Toulouse, France., Frongia C; Université de Toulouse, ITAV-USR3505, F-31106 Toulouse, France.; CNRS, ITAV-USR3505, F-31106 Toulouse, France., Clouvel G; Imagine Optic Orsay F-91400, France., Ducommun B; Université de Toulouse, ITAV-USR3505, F-31106 Toulouse, France.; CNRS, ITAV-USR3505, F-31106 Toulouse, France.; CHU de Toulouse, F-31059 Toulouse, France., Lorenzo C; Université de Toulouse, ITAV-USR3505, F-31106 Toulouse, France.; CNRS, ITAV-USR3505, F-31106 Toulouse, France.

Publikováno v: Scientific reports [Sci Rep] 2015 Nov 18; Vol. 5, pp. 16898. Date of Electronic Publication: 2015 Nov 18.