Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique

Autor: Daniel Ladant, Marilyne Davi, Gouzel Karimova, Scot P. Ouellette

Publikováno v: Current protocols in molecular biology
Current protocols in molecular biology, Wiley, 2017, 118, pp.20.12.1-20.12.24. ⟨10.1002/cpmb.36⟩
Current Protocols in Molecular Biology
Current Protocols in Molecular Biology, 2017, 118, pp.20.12.1-20.12.24. ⟨10.1002/cpmb.36⟩

The bacterial two-hybrid (BACTH, for "Bacterial Adenylate Cyclase-based Two-Hybrid") technique is a simple and fast genetic approach to analyze protein-protein interactions in vivo. In this system, the proteins of interest are genetically fused to tw

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27c585240cadd70c79abdbcc7c66e044
https://hal-pasteur.archives-ouvertes.fr/pasteur-02879386

α7 Neuronal Nicotinic Acetylcholine Receptors Are Negatively Regulated by Tyrosine Phosphorylation and Src-Family Kinases

Autor: Christian Fuhrer, Jean-Charles Hoda, R. Ogier, Andreas Wiesner, Mario Raggenbass, Geraldine Allaman, Eric Charpantier, Dominik Feuerbach, Daniel Bertrand, Kyung-Hye Huh

Publikováno v: Journal of Neuroscience, Vol. 25, No 43 (2005) pp. 9836-9849

Nicotine, a component of tobacco, is highly addictive but possesses beneficial properties such as cognitive improvements and memory maintenance. Involved in these processes is the neuronal nicotinic acetylcholine receptor (nAChR) α7, whose activatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4537d4e37a7df3b06ff3f56ecf5cbbe2
https://doi.org/10.1523/jneurosci.3497-05.2005

Positional cloning of the APECED gene

Autor: Stylianos E. Antonarakis, Kazuhiko Kawasaki, Maarit Heino, Hamish S. Scott, Nobuyoshi Shimizu, Shuichi Asakawa, Maria D. Lalioti, Kentaro Nagamine, Primus E. Mullis, Shinsei Minoshima, Fumiaki Ito, Pärt Peterson, Kai Krohn, Jun Kudoh

Publikováno v: Scopus-Elsevier
Nature Genetics, Vol. 17, No 4 (1997) pp. 393-398

Autoimmune polyglandular syndrome type I (APS 1, also called APECED) is an autosomal-recessive disorder that maps to human chromosome 21q22.3 between markers D21S49 and D21S171 by linkage studies. We have isolated a novel gene from this region, AIRE

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd8a6c8a8e5b14253107096e4d48c14b
https://doi.org/10.1038/ng1297-393

Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination

Autor: Pierre Cosson, Steve J. Charette, Sophie Cornillon

Publikováno v: Journal of Biotechnology, Vol. 122, No 1 (2006) pp. 1-4

Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31a59a78e072523a683bf9580ce8c67d
https://archive-ouverte.unige.ch/unige:39540

Production of reagents and optimization of methods for studying calmodulin-binding proteins

Autor: Thierry Soldati, Bettina Ulbricht

Publikováno v: Protein Expression and Purification, Vol. 15, No 1 (1999) pp. 24-33
Protein Expression and Purification

Owing to subtle but potentially crucial structural and functional differences between calmodulin (CaM) of different species, the biochemical study of low-affinity CaM-binding proteins from Dictyostelium discoideum likely necessitates the use of CaM f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4493ec77afc6168650500fe46a3b6b3
https://archive-ouverte.unige.ch/unige:18927