Zobrazeno 1 - 10
of 76
pro vyhledávání: '"Clifton K Fagerquist"'
Autor:
Jihyun Park, Clifton K Fagerquist
Publikováno v:
PLoS ONE, Vol 19, Iss 5, p e0299287 (2024)
Matrix-assisted laser desorption/ionization time-of-flight-time-of-flight (MALDI-TOF-TOF) tandem mass spectrometry (MS/MS) is a rapid technique for identifying intact proteins from unfractionated mixtures by top-down proteomic analysis. MS/MS allows
Externí odkaz:
https://doaj.org/article/f7e5b50d3876443691f46923ffc36d38
Autor:
Clifton K Fagerquist, Claire E Dodd
Publikováno v:
PLoS ONE, Vol 16, Iss 11, p e0260650 (2021)
Fourteen proteins produced by three pathogenic Escherichia coli strains were identified using antibiotic induction, MALDI-TOF-TOF tandem mass spectrometry (MS/MS) and top-down proteomic analysis using software developed in-house. Host proteins as wel
Externí odkaz:
https://doaj.org/article/76d62cb3da994dbb9315481023291d9b
Publikováno v:
MethodsX, Vol 6, Iss , Pp 815-826 (2019)
The method describes a step-by-step process for analysis of putative Shiga toxin-producing Escherichia coli (STEC) for expression of Shiga toxin (Stx). The technique utilizes antibiotic induction, mass spectrometry and top-down/middle-down proteomic
Externí odkaz:
https://doaj.org/article/95f420bc7d0c40fa8c8c851bfd018633
Autor:
Clifton K. Fagerquist
Publikováno v:
Microbiological Identification using MALDI‐TOF and Tandem Mass Spectrometry. :71-96
Publikováno v:
Frontiers in Nutrition, Vol 5 (2018)
We performed proteolytic surface-shaving with trypsin on three strains/sevovars of Salmonella enterica enterica (SEE): Newport, Kentucky, and Thompson. Surfaced-exposed proteins of live bacterial cells were digested for 15 min. A separate 20 h re-dig
Externí odkaz:
https://doaj.org/article/d2e1eaa0f091404e88dc9b83a71b0f3e
Publikováno v:
Rapid Communications in Mass Spectrometry. 37
Publikováno v:
International Journal of Mass Spectrometry. 489:117051
Publikováno v:
Microorganisms, Vol 7, Iss 11, p 488 (2019)
Shiga-toxin-producing Escherichia coli (STEC) are a burden on agriculture and a threat to public health. Rapid methods are needed to identify STEC strains and characterize the Shiga toxin (Stx) they produce. We analyzed three STEC strains for Stx exp
Externí odkaz:
https://doaj.org/article/36c1b152acca446398e2e983024660b0
Autor:
Clifton K, Fagerquist, Ernesto, Rojas
Publikováno v:
Journal of visualized experiments : JoVE. (171)
This protocol identifies the immunity proteins of the bactericidal enzymes: colicin E3 and bacteriocin, produced by a pathogenic Escherichia coli strain using antibiotic induction, and identified by MALDI-TOF-TOF tandem mass spectrometry and top-down
Autor:
Beatriz Quiñones, Jaszemyn C. Yambao, Bertram G. Lee, Clifton K. Fagerquist, William J. Zaragoza
Publikováno v:
International Journal of Mass Spectrometry. 438:1-12
A plasmid-borne factor (hypothetical protein) was identified in three Shiga toxin-producing Escherichia coli O113:H21 strains using antibiotic-induction, MALDI-TOF-TOF tandem mass spectrometry (MS/MS), post-source decay (PSD) and top-down proteomic a