Zobrazeno 1 - 10
of 103
pro vyhledávání: '"Clifford J, Unkefer"'
Autor:
Julian C.-H. Chen, Clifford J. Unkefer
Publikováno v:
IUCrJ, Vol 4, Iss 1, Pp 72-86 (2017)
The Protein Crystallography Station (PCS), located at the Los Alamos Neutron Scattering Center (LANSCE), was the first macromolecular crystallography beamline to be built at a spallation neutron source. Following testing and commissioning, the PCS us
Externí odkaz:
https://doaj.org/article/b5767f08f84342ea97660b09b033b27a
Publikováno v:
Acta Crystallographica Section F Structural Biology Communications. 78:177-184
A structure–function characterization of Synechococcus elongatus enolase (SeEN) is presented, representing the first structural report on a cyanobacterial enolase. X-ray crystal structures of SeEN in its apoenzyme form and in complex with phosphoen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4e54df60b53d4809d2b9865ca0aeb99
https://doi.org/10.1107/s2053230x22003612
https://doi.org/10.1107/s2053230x22003612
Publikováno v:
PLoS Computational Biology, Vol 14, Iss 2, p e1005972 (2018)
Mycolactone is the exotoxin produced by Mycobacterium ulcerans and is the virulence factor behind the neglected tropical disease Buruli ulcer. The toxin has a broad spectrum of biological effects within the host organism, stemming from its interactio
Externí odkaz:
https://doaj.org/article/9343a1d15f8e4a7a897ba8afa8e2e86f
Autor:
Naresh Pandey, Steffi A. Davison, Malathy Krishnamurthy, Daniel S. Trettel, Chien-Chi Lo, Shawn Starkenburg, Katherine L. Wozniak, Theresa L. Kern, Sean D. Reardon, Clifford J. Unkefer, Scott P. Hennelly, Taraka Dale
Publikováno v:
ACS synthetic biology. 11(10)
Engineered microbes can be used for producing value-added chemicals from renewable feedstocks, relieving the dependency on nonrenewable resources such as petroleum. These microbes often are composed of synthetic metabolic pathways; however, one major
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e76748d6c16d70d22106556b8dd28af
https://pubmed.ncbi.nlm.nih.gov/36130255
https://pubmed.ncbi.nlm.nih.gov/36130255
Autor:
Clifford J. Unkefer
Pyrroloquinoline quinone (PQQ) biosynthesis is complicated by the fact that it is synthesized in the cytoplasm and must then find its way to the periplasm where the active enzyme/cofactor complex is assembled. Clearly the a-nitrogen of tyrosine was n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::82d7cb992805a80e4bf789b0e91bbca2
https://doi.org/10.1201/9781003067009-14
https://doi.org/10.1201/9781003067009-14
Autor:
Brian Broom-Peltz, Javier M. González, Ricardo Marti-Arbona, Julian C.-H. Chen, Clifford J. Unkefer
Publikováno v:
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Three high-resolution X-ray crystal structures of malate dehydrogenase (MDH; EC 1.1.1.37) from the methylotroph Methylobacterium extorquens AM1 are presented. By comparing the structures of apo MDH, a binary complex of MDH and NAD+, and a ternary com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b51fd2e666d19cb26300ca9b9628db3f
https://doi.org/10.1107/s2053230x18011809
https://doi.org/10.1107/s2053230x18011809
Autor:
Clifford J. Unkefer, Julian C.-H. Chen
Publikováno v:
IUCrJ
IUCrJ, Vol 4, Iss 1, Pp 72-86 (2017)
IUCrJ, Vol 4, Iss 1, Pp 72-86 (2017)
This article highlights scientific and technical contributions from the Protein Crystallography Station at Los Alamos, the first purpose-built macromolecular crystallography station at a spallation neutron source.
The Protein Crystallography Sta
The Protein Crystallography Sta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c1945229859a96b0bd48292106a8edd
https://doi.org/10.1107/s205225251601664x
https://doi.org/10.1107/s205225251601664x
Autor:
Brian L. Mark, John-Paul Bacik, Timothy A. Whitehead, Justin R. Klesmith, Laura R. Jarboe, Clifford J. Unkefer, Ryszard Michalczyk
Publikováno v:
Journal of Biological Chemistry. 290:26638-26648
The most abundant carbohydrate product of cellulosic biomass pyrolysis is the anhydrosugar levoglucosan (1,6-anhydro-β-d-glucopyranose), which can be converted to glucose 6-phosphate by levoglucosan kinase (LGK). In addition to the canonical kinase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9a418e4cb5d082b366e7c3e46031b786
https://doi.org/10.1074/jbc.m115.674614
https://doi.org/10.1074/jbc.m115.674614
Autor:
Julian C.-H. Chen, Sophanit Mekasha, Vincent G. H. Eijsink, Jay C. Nix, Matthew J. Cuneo, Andrey Kovalevsky, Leighton Coates, Gustav Vaaje-Kolstad, Zarah Forsberg, Clifford J. Unkefer, John-Paul Bacik
Publikováno v:
Biochemistry, vol 56, iss 20
Bacik, J-P; Mekasha, S; Forsberg, Z; Kovalevsky, AY; Vaaje-Kolstad, G; Eijsink, VGH; et al.(2017). Neutron and Atomic Resolution X-ray Structures of a Lytic Polysaccharide Monooxygenase Reveal Copper-Mediated Dioxygen Binding and Evidence for N-Terminal Deprotonation. Biochemistry, 56(20), 2529-2532. doi: 10.1021/acs.biochem.7b00019. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2r94p31d
Bacik, J-P; Mekasha, S; Forsberg, Z; Kovalevsky, AY; Vaaje-Kolstad, G; Eijsink, VGH; et al.(2017). Neutron and Atomic Resolution X-ray Structures of a Lytic Polysaccharide Monooxygenase Reveal Copper-Mediated Dioxygen Binding and Evidence for N-Terminal Deprotonation. Biochemistry, 56(20), 2529-2532. doi: 10.1021/acs.biochem.7b00019. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2r94p31d
A 1.1 Å resolution, room-temperature X-ray structure and a 2.1 Å resolution neutron structure of a chitin-degrading lytic polysaccharide monooxygenase domain from the bacterium Jonesia denitrificans (JdLPMO10A) show a putative dioxygen species equa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9eb25d0f0540ede96a16d5e0496122ac
https://escholarship.org/uc/item/2r94p31d
https://escholarship.org/uc/item/2r94p31d
Autor:
Richard T. Sayre, Matteo Pellegrini, José A. Olivares, Ian K. Blaby, Jan G. Jaworski, Timothy P. Devarenne, Daniel B. Anderson, Shawn R. Starkenburg, James W. Richardson, Judith K. Brown, Martin Sabarsky, Clifford J. Unkefer, Jon K. Magnuson, Jonathan E. Holladay, István Molnár, Pat J. Unkefer, Taraka Dale, C. Meghan Downes, Ellen A. Panisko, John B. Mott, Ivan Baxter, Ursula Goodenough, Margaret McCormick, David T. Fox, Kimberly L. Ogden, Michael Carleton, Scott N. Twary, Andrew T. Koppisch, Gary D. Stormo, Susan K. Dutcher, David Kramer, Munehiro Teshima, Mary S. Lipton, Rose Ann Cattolico, Joshua S. Yuan, Babetta L. Marrone, Juergen E. W. Polle
Publikováno v:
Unkefer, C J, Sayre, R T, Magnuson, J K, Anderson, D B, Baxter, I, Blaby, I K, Brown, J K, Carleton, M, Cattolico, R A, Dale, T, Devarenne, T P, Downes, C M, Dutcher, S K, Fox, D T, Goodenough, U, Jaworski, J, Holladay, J E, Kramer, D M, Koppisch, A T, Lipton, M S, Marrone, B L, McCormick, M, Molnár, I, Mott, J B, Ogden, K L, Panisko, E A, Pellegrini, M, Polle, J, Richardson, J W, Sabarsky, M, Starkenburg, S R, Stormo, G D, Teshima, M, Twary, S N, Unkefer, P J, Yuan, J S & Olivares, J A 2017, ' Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts ', Algal Research, vol. 22, pp. 187-215 . https://doi.org/10.1016/j.algal.2016.06.002
In 2010, when the National Alliance for Advanced Biofuels and Bioproducts (NAABB) consortium began, little was known about the molecular basis of algal biomass or oil production. Very few algal genome sequences were available and efforts to identify
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d4c81271409c53605ec01cf26f1e535
https://escholarship.org/uc/item/9wf7q7md
https://escholarship.org/uc/item/9wf7q7md