Immobilization and characterization of latex cysteine peptidases on different supports and application for cow’s milk protein hydrolysis

Autor: João P.B. Oliveira, Luciana R.B. Gonçalves, Kímberle P.S. Amorim, Bruna B. Pinheiro, Márcio V. Ramos, Pedro F.N. Souza, Jefferson S. Oliveira, Deborah C. Freitas, Cleverson D.T. Freitas

Publikováno v: Process Biochemistry. 117:180-190

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3aa49d199e039858d5fcf6f18288907d
https://doi.org/10.1016/j.procbio.2022.04.005

Serine carboxypeptidases from the carnivorous plant Nepenthes mirabilis: Partial characterization and heterologous expression

Autor: Camila T.M.N. Porfírio, Pedro F.N. Souza, Márcio V. Ramos, Francisco A.P. Campos, Samuel F. Freitas, João P.B. Oliveira, Gilvan P. Furtado, José S.S. Barbosa, Thalia L. Frota, Celso S. Nagano, Rodolpho G.G. Silva, Ghulam Hussain, Cleverson D.T. Freitas

Publikováno v: International Journal of Biological Macromolecules. 198:77-86

This study aimed to partially characterize the three main serine carboxypeptidases (SCP3, SCP20, and SCP47) from Nepenthes mirabilis. Furthermore, one peptidase (SCP3) was chosen for further heterologous expression in Escherichia coli Shuffle®T7. SC

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::889558a63ae6746716b57541b3124f55
https://doi.org/10.1016/j.ijbiomac.2021.12.104

Insecticidal Compound from Himatanthus drasticus Latex against Cowpea Infestation by Callosobruchus maculatus (Coleoptera: Chrysomelidae)

Autor: Alison B. da Silva, José Francisco de Carvalho Gonçalves, Ayrles F.B. Silva, Márcio V. Ramos, Paulo Riceli Vasconcelos Ribeiro, Otília Deusdênia L. Pessoa, Brandon Ferraz E Sousa, Cleverson D.T. Freitas, Francimauro S. Morais, Emanuel Alves Bezerra, Diego P. Souza, Kirley Marques Canuto

Publikováno v: Journal of Agricultural and Food Chemistry. 69:5049-5058

Vigna unguiculata is an important source of proteins and energy for humans and animals. However, postharvest losses caused by Callosobruchus maculatus can reach from 20 to 100% of stored seeds. In this study, the insecticide potential of compounds ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::abb4d4d0951b8ee654cc8aa806896c6f
https://doi.org/10.1021/acs.jafc.1c01177

ACE2-derived peptides interact with the RBD domain of SARS-CoV-2 spike glycoprotein, disrupting the interaction with the human ACE2 receptor

Autor: Leandro P. Bezerra, Pedro F.N. Souza, Jose T.A. Oliveira, Cleverson D.T. Freitas, Jackson L. Amaral, Valder N. Freire, Francisco E.S. Lopes

Publikováno v: Journal of Biomolecular Structure and Dynamics
Journal of Biomolecular Structure & Dynamics
article-version (VoR) Version of Record

Vaccines could be the solution to the current SARS-CoV-2 outbreak. However, some studies have shown that the immunological memory only lasts three months. Thus, it is imperative to develop pharmacological treatments to cope with COVID-19. Here, the i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b987b3b596a12dd9b7a9f029c5f19bd0
https://doi.org/10.1080/07391102.2020.1871415

Standardized production of a homogeneous latex enzyme source overcoming seasonality and microenvironmental variables

Autor: Raphael Alves Coelho, Márcio V. Ramos, Sandro Rios Silveira, Cleverson D.T. Freitas, Brandon Ferraz E Sousa, Diego P. Souza, José Vitor Lima-Filho, Jefferson Soares de Oliveira, Pedro Abílio Vieira Rocha Júnior, Laura Maria Isabel Lopez

Publikováno v: Preparative Biochemistry & Biotechnology. 51:375-385

Calotropis procera produces a milky sap containing proteolytic enzymes. At low concentrations, they induce milk-clotting (60 µg/ml) and to dehair hides (0.05 and 0.1%). A protocol for obtaining the...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1806383f04da2edd303ee175c3517f88
https://doi.org/10.1080/10826068.2020.1818258