Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS)

Autor: Clement E. Blanchet, Adam Round, Haydyn D. T. Mertens, Kartik Ayyer, Melissa Graewert, Salah Awel, Daniel Franke, Katerina Dörner, Saša Bajt, Richard Bean, Tânia F. Custódio, Raphael de Wijn, E. Juncheng, Alessandra Henkel, Andrey Gruzinov, Cy M. Jeffries, Yoonhee Kim, Henry Kirkwood, Marco Kloos, Juraj Knoška, Jayanath Koliyadu, Romain Letrun, Christian Löw, Jana Makroczyova, Abhishek Mall, Rob Meijers, Gisel Esperanza Pena Murillo, Dominik Oberthür, Ekaterina Round, Carolin Seuring, Marcin Sikorski, Patrik Vagovic, Joana Valerio, Tamme Wollweber, Yulong Zhuang, Joachim Schulz, Heinrich Haas, Henry N. Chapman, Adrian P. Mancuso, Dmitri Svergun

Publikováno v: Communications Biology, Vol 6, Iss 1, Pp 1-11 (2023)

Abstract Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules are not as

Externí odkaz: https://doaj.org/article/bcf514c39af3436883cc2c8e5bf81aab

Controlled modulation of the dynamics of the Deinococcus grandis Dps N ‐terminal tails by divalent metals

Autor: João P. L. Guerra, Clement E. Blanchet, Bruno J. C. Vieira, João C. Waerenborgh, Nykola C. Jones, Søren Vrønning Hoffmann, Alice S. Pereira, Pedro Tavares

Publikováno v: Guerra, J P L, Blanchet, C E, Vieira, B J C, Waerenborgh, J C, Jones, N C, Hoffmann, S V, Pereira, A S & Tavares, P 2023, ' Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals ', Protein Science, vol. 32, no. 2, e4567 . https://doi.org/10.1002/pro.4567
Protein science 32(2), e4567 (2023). doi:10.1002/pro.4567

Protein science 32(2), e4567 (2023). doi:10.1002/pro.4567
DNA-binding proteins from starved cells (Dps) are small multifunctional nanocages expressed by prokaryotes in acute oxidative stress conditions or during the starvation-induced stationary

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::492852b37a4fcbb903006fa42e6492e8
https://doi.org/10.1002/pro.4567

Probing the existence of non-thermal Terahertz radiation induced changes of the protein solution structure

Autor: Manfred Roessle, Till Zickmantel, Andrey Yu. Gruzinov, Young-Hwa Song, Christian Rönnau, Dmitri I. Svergun, Siawosch Schewa, Martin A. Schroer, Clement E. Blanchet, Janine Mia Lahey-Rudolph

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Scientific Reports
Scientific reports 11(1), 22311 (2021). doi:10.1038/s41598-021-01774-6

Scientific reports 11(1), 22311 (2021). doi:10.1038/s41598-021-01774-6
During the last decades discussions were taking place on the existence of global, non-thermal structural changes in biological macromolecules induced by Terahertz (THz) radia

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99d6fc586cb543dd56172ac6c311480c
https://doaj.org/article/668ea2ef468f4b189896403cd4d47cbb

The Conformation of the N-Terminal Tails of

Autor: João P L, Guerra, Clement E, Blanchet, Bruno J C, Vieira, Ana V, Almeida, João C, Waerenborgh, Nykola C, Jones, Søren V, Hoffmann, Pedro, Tavares, Alice S, Pereira

Publikováno v: International journal of molecular sciences. 23(9)

DNA-binding proteins from starved cells (Dps) are homododecameric nanocages, with N- and C-terminal tail extensions of variable length and amino acid composition. They accumulate iron in the form of a ferrihydrite mineral core and are capable of bind

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1e4fe334147ee5e001e5ab790a8f617f
https://pubmed.ncbi.nlm.nih.gov/35563263

Hallmarks of Alpha- And Betacoronavirus non-structural protein 7+8 complexes

Autor: Dmitri I. Svergun, John Ziebuhr, Kristina Lorenzen, Boris Krichel, Renato Zenobi, Martin Koehler, Clement E. Blanchet, Robin Schubert, Charlotte Uetrecht, Ramakanth Madhugiri, L. Brings, Ganesh Bylapudi, C. Schmidt

Publikováno v: Science Advances, 7 (10)
Science Advances
bioRxiv
article-version (status) pre
article-version (number) 2
Science advances 7(10), eabf1004 (1-15) (2021). doi:10.1126/sciadv.abf1004

Coronaviral nsp7+8 complexes follow species- and stoichiometry-specific assembly pathways determined by few amino acid changes.
Coronaviruses infect many different species including humans. The last two decades have seen three zoonotic coronavir

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c368da939228739887e5639c8f871ea8
https://hdl.handle.net/20.500.11850/474238