Activation of cytochrome c to a peroxidase compound I-type intermediate by H2O2: relevance to redox signalling in apoptosis

Autor: Peter Wardman, Clare M. Jones, Andrew Lawrence, Mark J. Burkitt

Publikováno v: Biochemical Society Symposia. 71:97-106

The release of cytochrome c from mitochondria during apoptosis results in the enhanced production of superoxide radicals, which are converted to H2O2 by Mn-superoxide dismutase. We have been concerned with the role of cytochrome c/H2O2 in the inducti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c9c3b8e61023e87dc1e2be89addcf08
https://doi.org/10.1042/bss0710097

EPR detection of the unstable tert-butylperoxyl radical adduct of the spin trap 5,5-dimethyl-1-pyrroline N-oxide: a combined spin-trapping and continuous-flow investigation

Autor: Mark J. Burkitt, Clare M. Jones

Publikováno v: Journal of the Chemical Society, Perkin Transactions 2. :2044-2051

The EPR spin-trapping technique has been applied extensively to the detection of organic peroxyl radicals in biological systems. The most widely used spin trap is 5,5-dimethyl-1-pyrroline N-oxide (DMPO), of which adducts displaying EPR signals with a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2920e0b71db4003cf0c8b6ab367a1ae3
https://doi.org/10.1039/b207709b

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Autor: Peter Wardman, Mark J. Burkitt, Borivoj Vojnovic, Steven A. Everett, Kantilal B. Patel, Andrew Lawrence, Clare M. Jones

Publikováno v: Journal of Fluorescence. 12:65-68

Lucigenin (LC 2+ , bis-N-methylacridinium) and 28,78 -dichlorofluorescin (DCFH2) are widely used as chemiluminescent or fluorescent probes for cellular oxidative stress, to reflect levels of superoxide (O2 ?2 ) and hydrogen peroxide, respectively. We

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::763381a3e8192880912c0f00f3b1f5f7
https://doi.org/10.1023/a:1015363220266

Mechanism of the antioxidant to pro-oxidant switch in the behavior of dehydroascorbate during LDL oxidation by copper(II) ions

Autor: Clare M. Jones, Jessica D. del Rio, Rebecca A. Patterson, Lynda K. Harris, Elizabeth T.M. Horsley, Mark J. Burkitt, David S. Leake, Nicola J. Moss

Publikováno v: Horsley, E T M, Burkitt, M J, Jones, C M, Patterson, R A, Harris, L K, Moss, N J, del Rio, J D & Leake, D S 2007, ' Mechanism of the antioxidant to pro-oxidant switch in the behavior of dehydroascorbate during LDL oxidation by copper(II) ions ', Archives of Biochemistry and Biophysics, vol. 465, no. 2, pp. 303-314 . https://doi.org/10.1016/j.abb.2007.07.005

Oxidised low density lipoprotein (LDL) may be involved in the pathogenesis of atherosclerosis. We have therefore investigated the mechanisms underlying the antioxidant/pro-oxidant behavior of dehydroascorbate, the oxidation product of ascorbic acid,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ec45dbc890a02300e06bea53e1b07eb
https://pubmed.ncbi.nlm.nih.gov/17689484

EPR spin-trapping evidence for the direct, one-electron reduction of tert-butylhydroperoxide to the tert-butoxyl radical by copper(II): paradigm for a previously overlooked reaction in the initiation of lipid peroxidation

Autor: Mark J. Burkitt, Clare M. Jones

Publikováno v: Journal of the American Chemical Society. 125(23)

Lipid peroxidation is often initiated using Cu(II) ions. It is widely assumed that Cu(II) oxidizes preformed lipid hydroperoxides to peroxyl radicals, which propagate oxidation of the parent fatty acid via hydrogen atom abstraction. However, the oxid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9fbaca36b9fc69d6fbc135f00ab8ecb
https://pubmed.ncbi.nlm.nih.gov/12783547