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pro vyhledávání: '"Clare E Buckley"'
Autor:
Clare E. Buckley, Daniel St Johnston
Publikováno v:
Nature Reviews Molecular Cell Biology. 23:559-577
Individual cells within de novo polarising tubes and cavities must integrate their forming apical domains into a centralised apical membrane initiation site (AMIS). This is necessary to enable organised lumen formation within multi-cellular tissue. D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33437acb436bd93e743f13f5592e5718
https://www.repository.cam.ac.uk/handle/1810/340328
https://www.repository.cam.ac.uk/handle/1810/340328
Autor:
Helena A. Crellin, Clare E. Buckley
Publikováno v:
Cells, tissues, organs.
The initiation of apical-basal (AB) polarity and the process of mitotic cell division are both characterised by the generation of specialised plasma membrane and cortical domains. These are generated using shared mechanisms, such as asymmetric protei
Autor:
Clare E, Buckley, Daniel, St Johnston
Publikováno v:
Nature reviews. Molecular cell biology. 23(8)
Epithelial cells are the most common cell type in all animals, forming the sheets and tubes that compose most organs and tissues. Apical-basal polarity is essential for epithelial cell form and function, as it determines the localization of the adhes
Using the zebrafish neural tube as a model, we uncover thein vivomechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that NOK/Pals1/Mpp5a and Rab11a play a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f51140dbc87d77df808b467b8786a414
https://www.repository.cam.ac.uk/handle/1810/313573
https://www.repository.cam.ac.uk/handle/1810/313573
Publikováno v:
Development. 147
Using the zebrafish neural tube as a model, we uncover the in vivo mechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that Mpp5a and Rab11a play a dual rol
Autor:
Clare E. Buckley
This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an “anchor” protein and a “bait” protein. Proteins can therefore
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::955092b8fc291c75629d15926160db06
https://doi.org/10.17504/protocols.io.bp3jmqkn
https://doi.org/10.17504/protocols.io.bp3jmqkn
Autor:
Clare E, Buckley
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1920
This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an "anchor" protein and a "bait" protein. Proteins can therefore be dire
Autor:
Rachel E. Moore, Anna Reade, Orion D. Weiner, Jonathan D.W. Clarke, Anna R. Goldberg, Clare E. Buckley
Publikováno v:
Developmental Cell
Developmental cell, vol 36, iss 1
Buckley, C, Moore, R, Reade, A, Goldberg, A R, Weiner, O D & Clarke, J 2016, ' Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo ', Developmental Cell, vol. 36, no. 1, pp. 117–126 . https://doi.org/10.1016/j.devcel.2015.12.011
Buckley, CE; Moore, RE; Reade, A; Goldberg, AR; Weiner, OD; & Clarke, JDW. (2016). Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Developmental Cell, 36(1), 117-126. doi: 10.1016/j.devcel.2015.12.011. UCSF: Retrieved from: http://www.escholarship.org/uc/item/1qw221pt
Developmental cell, vol 36, iss 1
Buckley, C, Moore, R, Reade, A, Goldberg, A R, Weiner, O D & Clarke, J 2016, ' Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo ', Developmental Cell, vol. 36, no. 1, pp. 117–126 . https://doi.org/10.1016/j.devcel.2015.12.011
Buckley, CE; Moore, RE; Reade, A; Goldberg, AR; Weiner, OD; & Clarke, JDW. (2016). Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Developmental Cell, 36(1), 117-126. doi: 10.1016/j.devcel.2015.12.011. UCSF: Retrieved from: http://www.escholarship.org/uc/item/1qw221pt
Summary We demonstrate the utility of the phytochrome system to rapidly and reversibly recruit proteins to specific subcellular regions within specific cells in a living vertebrate embryo. Light-induced heterodimerization using the phytochrome system