Apical–basal polarity and the control of epithelial form and function

Autor: Clare E. Buckley, Daniel St Johnston

Publikováno v: Nature Reviews Molecular Cell Biology. 23:559-577

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6870680359c19364a7c4ca8da57d9f6c
https://doi.org/10.1038/s41580-022-00465-y

Using optogenetics to investigate the shared mechanisms of apical-basal polarity and mitosis

Autor: Helena A. Crellin, Clare E. Buckley

Publikováno v: Cells, tissues, organs.

The initiation of apical-basal (AB) polarity and the process of mitotic cell division are both characterised by the generation of specialised plasma membrane and cortical domains. These are generated using shared mechanisms, such as asymmetric protei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17c9fc14ff00449688f510507f0a36ef
https://pubmed.ncbi.nlm.nih.gov/36599311

Coordinated assembly and release of adhesions builds apical junctional belts during de novo polarisation of an epithelial tube

Autor: Charlotte A. Williams, Jonathan D.W. Clarke, Andrew C. Symonds, Clare E. Buckley

Using the zebrafish neural tube as a model, we uncover thein vivomechanisms allowing the generation of two opposing apical epithelial surfaces within the centre of an initially unpolarised, solid organ. We show that NOK/Pals1/Mpp5a and Rab11a play a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f51140dbc87d77df808b467b8786a414
https://www.repository.cam.ac.uk/handle/1810/313573

Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos v1

Autor: Clare E. Buckley

This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an “anchor” protein and a “bait” protein. Proteins can therefore

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::955092b8fc291c75629d15926160db06
https://doi.org/10.17504/protocols.io.bp3jmqkn

Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo

Autor: Rachel E. Moore, Anna Reade, Orion D. Weiner, Jonathan D.W. Clarke, Anna R. Goldberg, Clare E. Buckley

Publikováno v: Developmental Cell
Developmental cell, vol 36, iss 1
Buckley, C, Moore, R, Reade, A, Goldberg, A R, Weiner, O D & Clarke, J 2016, ' Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo ', Developmental Cell, vol. 36, no. 1, pp. 117–126 . https://doi.org/10.1016/j.devcel.2015.12.011
Buckley, CE; Moore, RE; Reade, A; Goldberg, AR; Weiner, OD; & Clarke, JDW. (2016). Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Developmental Cell, 36(1), 117-126. doi: 10.1016/j.devcel.2015.12.011. UCSF: Retrieved from: http://www.escholarship.org/uc/item/1qw221pt

Summary We demonstrate the utility of the phytochrome system to rapidly and reversibly recruit proteins to specific subcellular regions within specific cells in a living vertebrate embryo. Light-induced heterodimerization using the phytochrome system

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::204e7f21adb26cf9fef03207822aac19
http://europepmc.org/articles/PMC4712025