Zobrazeno 1 - 10
of 84
pro vyhledávání: '"Clara L. Kielkopf"'
Autor:
Anant A. Agrawal, Enea Salsi, Rakesh Chatrikhi, Steven Henderson, Jermaine L. Jenkins, Michael R. Green, Dmitri N. Ermolenko, Clara L. Kielkopf
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-14 (2016)
The pre-mRNA splicing factor U2AF65 recognizes 3′ splice sites in human gene transcripts, but the details are not fully understood. Here, the authors report U2AF65 structures and single molecule FRET that reveal mechanistic insights into splice sit
Externí odkaz:
https://doaj.org/article/c1b792d28aba471e81c175eb71e73c9e
Autor:
Eliezra Glasser, Debanjana Maji, Giulia Biancon, Anees Mohammed Keedakkatt Puthenpeedikakkal, Chapin E Cavender, Toma Tebaldi, Jermaine L Jenkins, David H Mathews, Stephanie Halene, Clara L Kielkopf
Publikováno v:
Nucleic acids research. 50(9)
The essential pre-mRNA splicing factor U2AF2 (also called U2AF65) identifies polypyrimidine (Py) tract signals of nascent transcripts, despite length and sequence variations. Previous studies have shown that the U2AF2 RNA recognition motifs (RRM1 and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::650d69018f920925266f5e33b0615612
https://pubmed.ncbi.nlm.nih.gov/35524551
https://pubmed.ncbi.nlm.nih.gov/35524551
Autor:
Kholiswa M. Laird, Dmitri N. Ermolenko, Chandani Warnasooriya, Callen F. Feeney, Clara L. Kielkopf
Publikováno v:
Nucleic Acids Research
An essential heterodimer of the U2AF1 and U2AF2 pre-mRNA splicing factors nucleates spliceosome assembly at polypyrimidine (Py) signals preceding the major class of 3′ splice sites. U2AF1 frequently acquires an S34F-encoding mutation among patients
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::710bfe1305d01e58a957e81db9a4e59e
https://doi.org/10.1093/nar/gkaa293
https://doi.org/10.1093/nar/gkaa293
Autor:
Justin Galardi, Victoria N. Bela, Nazish Jeffery, Xueyang He, Eliezra Glasser, Sarah Loerch, Jermaine L. Jenkins, Mary J. Pulvino, Paul L. Boutz, Clara L. Kielkopf
In the early stages of spliceosome assembly, the 3’ splice site is recognized by sequential complexes of U2AF2 with SF1 followed by the SF3B1 subunit of the U2 small nuclear ribonucleoprotein particle. The U2AF2 – SF1 interface comprises a U2AF h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ce9d2eb5b562859ca34ba7cf7135c5a4
https://doi.org/10.1101/2022.01.09.475535
https://doi.org/10.1101/2022.01.09.475535
Autor:
Justin W. Galardi, Victoria N. Bela, Nazish Jeffery, Xueyang He, Eliezra Glasser, Sarah Loerch, Jermaine L. Jenkins, Mary J. Pulvino, Paul L. Boutz, Clara L. Kielkopf
Publikováno v:
The Journal of biological chemistry. 298(8)
During spliceosome assembly, the 3' splice site is recognized by sequential U2AF2 complexes, first with Splicing Factor 1 (SF1) and second by the SF3B1 subunit of the U2 small nuclear ribonuclear protein particle. The U2AF2-SF1 interface is well char
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c69d3058259b72514ea915059bd48c3d
https://pubmed.ncbi.nlm.nih.gov/35780835
https://pubmed.ncbi.nlm.nih.gov/35780835
Publikováno v:
Cold Spring Harbor protocols. 2021(12)
In immunoblotting (western blotting), proteins are first separated by SDS-PAGE and then transferred electrophoretically from the gel onto a support membrane that binds proteins tightly. After the unreacted binding sites of the membrane are blocked to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::989fc2b8e564d0673559f2c458150b3b
https://pubmed.ncbi.nlm.nih.gov/34853123
https://pubmed.ncbi.nlm.nih.gov/34853123
Publikováno v:
Cold Spring Harbor protocols. 2021(12)
Many variations of the original Coomassie Brilliant Blue staining procedure are in use. This protocol describes some selected variations on the standard procedure that give comparable and consistent staining results for proteins in the 20- to 200-kDa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b0fa5d51d426a2a48f25d4fe7201222
https://pubmed.ncbi.nlm.nih.gov/34853121
https://pubmed.ncbi.nlm.nih.gov/34853121
Autor:
Clara L. Kielkopf, Jermaine L. Jenkins, Stephanie Halene, Eliezra Glasser, Mary J. Pulvino, Giulia Biancon, Debanjana Maji, Chapin E. Cavender, Anees Mohammed Keedakkatt Puthenpeedikakkal, Toma Tebaldi, David H. Mathews
The essential pre-mRNA splicing factor U2AF2 (also called U2AF65) identifies polypyrimidine (Py) tract signals of nascent transcripts, despite length and sequence variations. Previous studies have shown that the U2AF2 RNA recognition motifs (RRM1 and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::114708a8a54b760a77eaf7db4e27a94d
https://doi.org/10.1101/2021.09.27.462007
https://doi.org/10.1101/2021.09.27.462007
Autor:
Mary J. Pulvino, Zackary Falls, Matthew J. Walter, Rakesh Chatrikhi, Andrew J. MacRae, Jermaine L. Jenkins, Callen F. Feeney, Clara L. Kielkopf, Melissa S. Jurica, William W. Brennessel, Sumit Rai, Georgios Alachouzos, Alison J. Frontier, Timothy A. Graubert, Ram Samudrala
Publikováno v:
Cell chemical biology, vol 28, iss 8
Cell Chem Biol
Cell Chem Biol
Dysregulated pre-mRNA splicing is an emerging Achilles heel of cancers and myelodysplasias. To expand the currently limited portfolio of small molecule drug leads, we screened for chemical modulators of the U2AF complex, which nucleates spliceosome a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::895a2cf142ec23caeae71ba14975f65e
https://escholarship.org/uc/item/2sk8s5gr
https://escholarship.org/uc/item/2sk8s5gr
Autor:
Justin R. Leach, Clara L. Kielkopf, Manuel Ares, Jermaine L. Jenkins, Nazish N. Jeffery, Jason Talkish, Steven W. Horner, Oarteze Hunter, Haller Igel
Publikováno v:
RNA (New York, N.Y.), vol 25, iss 8
Stable recognition of the intron branchpoint by the U2 snRNP to form the pre-spliceosome is the first ATP-dependent step of splicing. Genetic and biochemical data from yeast indicate that Cus2 aids U2 snRNA folding into the stem IIa conformation prio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::170b87f45406b98a50709f73b4db05b2
https://doi.org/10.1261/rna.070649.119
https://doi.org/10.1261/rna.070649.119