Dynamic NF-κB and E2F interactions control the priority and timing of inflammatory signalling and cell proliferation

Autor: John M Ankers, Raheela Awais, Nicholas A Jones, James Boyd, Sheila Ryan, Antony D Adamson, Claire V Harper, Lloyd Bridge, David G Spiller, Dean A Jackson, Pawel Paszek, Violaine Sée, Michael RH White

Publikováno v: eLife, Vol 5 (2016)

Dynamic cellular systems reprogram gene expression to ensure appropriate cellular fate responses to specific extracellular cues. Here we demonstrate that the dynamics of Nuclear Factor kappa B (NF-κB) signalling and the cell cycle are prioritised di

Externí odkaz: https://doaj.org/article/c93dc22ec0694d76b838a26b6974d89e

Dynamic analysis of stochastic transcription cycles.

Autor: Claire V Harper, Bärbel Finkenstädt, Dan J Woodcock, Sönke Friedrichsen, Sabrina Semprini, Louise Ashall, David G Spiller, John J Mullins, David A Rand, Julian R E Davis, Michael R H White

Publikováno v: PLoS Biology, Vol 9, Iss 4, p e1000607 (2011)

In individual mammalian cells the expression of some genes such as prolactin is highly variable over time and has been suggested to occur in stochastic pulses. To investigate the origins of this behavior and to understand its functional relevance, we

Externí odkaz: https://doaj.org/article/188cf6c3240644bba2dec08fe24b29a3

Transcription factor Pit-1 affects transcriptional timing in the dual-promoter human prolactin gene

Autor: Michael R. H. White, Sabrina Semprini, Claire V. Harper, Nicholas Jones, David G. Spiller, Karen Featherstone, Anne V McNamara, Antony A Adamson, Hiroshi Momiji, Bärbel Finkenstädt, John J. Mullins, Lee M. Dunham, David A. Rand, Raheela Awais, Julian R. E. Davis

Publikováno v: Endocrinology
McNamara, A V, Awais, R, Momiji, H, Dunham, L, Featherstone, K, Harper, C V, Adamson, A, Semprini, S, Jones, N A, Spiller, D G, Mullins, J J, Finkenstädt, B F, Rand, D, White, M R H & Davis, J R E 2021, ' Transcription factor Pit-1 affects transcriptional timing in the dual-promoter human prolactin gene ', Endocrinology, vol. 162, no. 4, bqaa249 . https://doi.org/10.1210/endocr/bqaa249
Mcnamara, A V, Awais, R, Momiji, H, Dunham, L, Featherstone, K, Harper, C V, Adamson, A A, Semprini, S, Jones, N A, Spiller, D G, Mullins, J J, Finkenstädt, B F, Rand, D, White, M R H & Davis, J R E 2021, ' Transcription factor Pit-1 affects transcriptional timing in the dual-promoter human prolactin gene ', Endocrinology . https://doi.org/10.1210/endocr/bqaa249

Gene transcription occurs in short bursts interspersed with silent periods, and these kinetics can be altered by promoter structure. The effect of alternate promoter architecture on transcription bursting is not known. We studied the human prolactin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d3d33d6a6a1eeac0e7aa4fe834e169c
http://wrap.warwick.ac.uk/146862/7/WRAP-Transcription-factor-Pit-1-affects-transcriptional-timing-dual-promoter-human-prolactin-gene-Rand-2021.pdf

Multiplexing information flow through dynamic signalling systems

Autor: Claire V. Harper, Louise Ashall, Giorgos Minas, Michael R. H. White, Dan J. Woodcock, David A. Rand

Publikováno v: PLoS Computational Biology, Vol 16, Iss 8, p e1008076 (2020)
PLoS Computational Biology

We consider how a signalling system can act as an information hub by multiplexing information arising from multiple signals. We formally define multiplexing, mathematically characterise which systems can multiplex and how well they can do it. While t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78fe48285c1eafb8d1eaedc1df1d252d
http://wrap.warwick.ac.uk/139682/7/WRAP-Multiplexing-information-flow-through-dynamic-signalling-systems-Rand-2020.pdf

Signal transduction controls heterogeneous NF-κB dynamics and target gene expression through cytokine-specific refractory states

Autor: Pawel Paszek, Polly Downton, David A. Rand, Dean A. Jackson, Claire V. Harper, Michael R. H. White, Lorraine Schmidt, William Rowe, Antony Adamson, Apolinar Maya-Mendoza, James Bagnall, David G. Spiller, Connie Lam, Christopher Boddington

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-14 (2016)
Adamson, A, Boddington, C, Downton, P, Rowe, W, Bagnall, J, Lam, C, Maya-Mendoza, A, Schmidt, L, Harper, C V, Spiller, D, Rand, D A, Jackson, D, White, M R H & Paszek, P 2016, ' Signal transduction controls heterogeneous NF-κB dynamics and target gene expression through cytokine-specific refractory states ', Nature Communications, vol. 7, 12057 . https://doi.org/10.1038/ncomms12057
Nature Communications

Cells respond dynamically to pulsatile cytokine stimulation. Here we report that single, or well-spaced pulses of TNFα (>100 min apart) give a high probability of NF-κB activation. However, fewer cells respond to shorter pulse intervals (
In b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78c03441fa90fdd82e87df576c5fa7d2
https://doi.org/10.1038/ncomms12057