Spatio-temporal based deep learning for rapid detection and identification of bacterial colonies through lens-free microscopy time-lapses.

Autor: Paul Paquin, Claire Durmort, Caroline Paulus, Thierry Vernet, Pierre R Marcoux, Sophie Morales

Publikováno v: PLOS Digital Health, Vol 1, Iss 10, p e0000122 (2022)

Detection and identification of pathogenic bacteria isolated from biological samples (blood, urine, sputum, etc.) are crucial steps in accelerated clinical diagnosis. However, accurate and rapid identification remain difficult to achieve due to the c

Externí odkaz: https://doaj.org/article/08f47077d4b94b638ce40c6f55479695

Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System

Autor: Claire Durmort, Giuseppe Ercoli, Elisa Ramos-Sevillano, Suneeta Chimalapati, Richard D. Haigh, Megan De Ste Croix, Katherine Gould, Jason Hinds, Yann Guerardel, Thierry Vernet, Marco Oggioni, Jeremy S. Brown

Publikováno v: mBio, Vol 11, Iss 2 (2020)

ABSTRACT The capsule is the dominant Streptococcus pneumoniae virulence factor, yet how variation in capsule thickness is regulated is poorly understood. Here, we describe an unexpected relationship between mutation of adcAII, which encodes a zinc up

Externí odkaz: https://doaj.org/article/3c6b97acfd444f4693bf02410dff2bee

New insights into histidine triad proteins: solution structure of a Streptococcus pneumoniae PhtD domain and zinc transfer to AdcAII.

Autor: Beate Bersch, Catherine Bougault, Laure Roux, Adrien Favier, Thierry Vernet, Claire Durmort

Publikováno v: PLoS ONE, Vol 8, Iss 11, p e81168 (2013)

Zinc (Zn(2+)) homeostasis is critical for pathogen host colonization and invasion. Polyhistidine triad (Pht) proteins, located at the surface of various streptococci, have been proposed to be involved in Zn(2+) homeostasis. The phtD gene, coding for

Externí odkaz: https://doaj.org/article/7e7127325e36445c8227de1e79431605

Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System

Autor: Megan De Ste Croix, Katherine A. Gould, Jeremy S. Brown, Giuseppe Ercoli, Yann Guérardel, Marco R. Oggioni, Richard D. Haigh, Suneeta Chimalapati, Thierry Vernet, Jason Hinds, Claire Durmort, Elisa Ramos-Sevillano

Publikováno v: mBio
mBio, American Society for Microbiology, 2020, 11 (2), ⟨10.1128/mBio.00445-20⟩
mBio, Vol 11, Iss 2, p e00445-20 (2020)
mBio, Vol 11, Iss 2 (2020)
mBio, 2020, 11 (2), ⟨10.1128/mBio.00445-20⟩

The Streptococcus pneumoniae capsule affects multiple interactions with the host including contributing to colonization and immune evasion. During infection, the capsule thickness varies, but the mechanisms regulating this are poorly understood. We h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::900bc029a123ff70114ea943309d7450
https://hal.univ-grenoble-alpes.fr/hal-02864335/document

One-Pot Two-Step Metabolic Labeling of Teichoic Acids and Direct Labeling of Peptidoglycan Reveals Tight Coordination of Both Polymers Inserted into Pneumococcus Cell Wall

Autor: Anne Marie Di Guilmi, Yung-Sing Wong, Thierry Vernet, J. Bonnet, Claire Durmort, André Zapun

Publikováno v: ACS Chemical Biology
ACS Chemical Biology, 2018, 13 (8), pp.2010-2015. ⟨10.1021/acschembio.8b00559⟩
ACS Chemical Biology, American Chemical Society, 2018, 13 (8), pp.2010-2015. ⟨10.1021/acschembio.8b00559⟩

International audience; A method for labeling teichoic acids in the human pathogen Streptococcus pneumoniae has been developed using a one-pot two-step metabolic labeling approach. The essential nutriment choline modified with an azido-group was inco

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d3d88ae753d01218f9a0d799e68ff2b
https://doi.org/10.1021/acschembio.8b00559