Redirecting substrate regioselectivity using engineered ΔN123-GBD-CD2 branching sucrases for the production of pentasaccharide repeating units of S. flexneri 3a, 4a and 4b haptens

Autor: Mounir Benkoulouche, Akli Ben Imeddourene, Louis-Antoine Barel, Guillaume Le Heiget, Sandra Pizzut, Hanna Kulyk, Floriant Bellvert, Sophie Bozonnet, Laurence A. Mulard, Magali Remaud-Siméon, Claire Moulis, Isabelle André

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Abstract The (chemo-)enzymatic synthesis of oligosaccharides has been hampered by the lack of appropriate enzymatic tools with requisite regio- and stereo-specificities. Engineering of carbohydrate-active enzymes, in particular targeting the enzyme a

Externí odkaz: https://doaj.org/article/ef2c88e7c7fc466baca624798a644eb8

The role of the C domain in the thermostability of GH70 enzymes investigated by domain swapping

Autor: Manon Molina, Thomas Prévitali, Claire Moulis, Gianluca Cioci, Magali Remaud-Siméon

Publikováno v: Amylase. 6:11-19

Sucrose-active enzymes belonging to the glycoside hydrolase (GH) family 70 are attractive tools for the synthesis of oligosaccharides, polysaccharides or glycoconjugates. However, their thermostability is an important issue for the development of rob

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d9839971c5d8f4141d2b2397080ea0f3
https://doi.org/10.1515/amylase-2022-0002

Exopolysaccharide (EPS) synthesis by Oenococcus oeni: from genes to phenotypes.

Autor: Maria Dimopoulou, Marlène Vuillemin, Hugo Campbell-Sills, Patrick M Lucas, Patricia Ballestra, Cécile Miot-Sertier, Marion Favier, Joana Coulon, Virginie Moine, Thierry Doco, Maryline Roques, Pascale Williams, Melina Petrel, Etienne Gontier, Claire Moulis, Magali Remaud-Simeon, Marguerite Dols-Lafargue

Publikováno v: PLoS ONE, Vol 9, Iss 6, p e98898 (2014)

Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available and 36 newly sequenced ones) provided an inventory of the genes potentially involved in exopo

Externí odkaz: https://doaj.org/article/c52a69d9a22c4ca2a3c26c5769edb0d3

A convergent chemoenzymatic strategy to deliver a diversity of Shigella flexneri serotype-specific O-antigen segments from a unique lightly protected tetrasaccharide core

Autor: Akli Ben Imeddourene, Guillaume Le Heiget, Claire Moulis, Isabelle André, Zhaoyu Hu, Nelly Monties, Laurence A. Mulard, Magali Remaud-Simeon, Mounir Benkoulouche, Louis-Antoine Barel, Catherine Guerreiro, Yann Le Guen

Publikováno v: Journal of Organic Chemistry
Journal of Organic Chemistry, 2021, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩
Journal of Organic Chemistry, American Chemical Society, 2020, 86 (3), pp.2058-2075. ⟨10.1021/acs.joc.0c00777⟩

International audience; Progress in glycoscience is strongly dependent on the availability of broadly diverse tailored-made, well-defined and often complex oligosaccharides. Herein, going beyond natural resources and aiming to circumvent chemical bou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9424a376996bb908628ac2d5c4f976e
https://hal.insa-toulouse.fr/hal-03020947/document

Convergent Chemoenzymatic Strategy to Deliver a Diversity of

Autor: Zhaoyu, Hu, Mounir, Benkoulouche, Louis-Antoine, Barel, Guillaume, Le Heiget, Akli, Ben Imeddourene, Yann, Le Guen, Nelly, Monties, Catherine, Guerreiro, Magali, Remaud-Siméon, Claire, Moulis, Isabelle, André, Laurence A, Mulard

Publikováno v: The Journal of organic chemistry. 86(3)

Progress in glycoscience is strongly dependent on the availability of broadly diverse tailor-made, well-defined, and often complex oligosaccharides. Herein, going beyond natural resources and aiming to circumvent chemical boundaries in glycochemistry

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fe7e8c5c8624fd9471ff197e5ae0344a
https://pubmed.ncbi.nlm.nih.gov/32700907