Výsledky vyhledávání - "Ciołak, Agata"

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Additional file 3 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Autor: Joachimiak, Paweł, Ciesiołka, Adam, Kozłowska, Emilia, Świtoński, Paweł M., Figura, Grzegorz, Ciołak, Agata, Adamek, Grażyna, Surdyka, Magdalena, Kalinowska-Pośka, Żaneta, Figiel, Maciej, Caron, Nicholas S., Hayden, Michael R., Fiszer, Agnieszka

Additional file 3: Fig. S3. Precise values describing WT/MUT ratio of ATXN3 in selected SCA3 cells. A) Table with mean %WT and %MUT values obtained using four types of SCA3 cell lines and two SNP assays (mean values from three biological replicates).

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Additional file 6 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 6: Fig. S6. Estimation of the total number of endogenous HTT transcripts per diploid genome. Results were obtained using HTT_SNP7 assay and cDNA from HD iPSCs and NSCs. Data were analyzed using unpaired t test. For all experiments pre

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Additional file 2 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 2: Fig. S2. Immunocytochemistry performed on SCA3 and HD neurons. Exemplary images of immunofluorescent staining of SCA3 neurons for MAP 2 and TUJ1, and HD neurons for DARPP32, TUJ1, MAP 2 and GAD67. DAPI was used for nuclei staining.

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Additional file 8 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 8: Fig. S8. Precise values describing WT/MUT ratio of HTT transgene in Hu128/21 mice. A) Table with mean %WT and %MUT values obtained using mice brain tissues and two ddPCR assays (mean values from three biological replicates). B-C) R

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Additional file 1 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 1: Fig. S1. Analyses of ATXN3 and HTT assays specificity. Results from ddPCR assays performed on seven samples with predefined ratios of WT/MUT ATXN3 (A) and HTT (B-C) plasmids (I—100% WT and 0% MUT; II—90% WT and 10% MUT; III—7

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Additional file 7 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 7: Fig. S7. Precise values describing WT/MUT ratio of HTT in isogenic controls to HD cells. A) Table with mean %WT and %corrMUT values obtained for lines and presented SNP assays (mean values from three biological replicates). B-C) Re

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Additional file 5 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 5: Fig. S5. RNA-Seq analysis of HTT reads performed on HD iPSCs and NSCs. A-B) Results from RNA-Seq are presented as a mean WT and MUT HTT transcript allele abundance, calculated based on reads from three HTT SNP regions (SNP2, SNP5 a

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Additional file 4 of Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Additional file 4: Fig. S4. Precise values describing WT/MUT ratio of HTT in selected HD cells. A) Table with mean %WT and %MUT values obtained using three types of HD cell lines and three SNP assays (mean values from three biological replicates). B-

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