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pro vyhledávání: '"Cindy L. Boerma"'
Autor:
Cindy L. Boerma, James R. Y. Rawson
Publikováno v:
Biochemistry. 15:588-592
The fraction of chloroplast DNA transcribed at different stages of chloroplast development in Euglena gracilis was measured by RNA-DNA hybridization. Euglena cells were grown in the dark in a heterotrophic medium to stationary phase and then transfer
Autor:
James R. Y. Rawson, Cindy L. Boerma
Publikováno v:
Biochemical and Biophysical Research Communications. 74:912-918
Chloroplast DNA from Euglena gracilis was separated in an alkaline CsC1 density gradient into a heavy and a light component. The heavy and the light DNA components each contain 18 percent self-complementary nucleotide sequences. Equal masses of the h
Publikováno v:
Gene. 3:191-209
Chloroplast ribosomal DNA from Euglena gracilis was partially purified, digested with restriction endonucleases Bam HI or Eco RI and cloned into bacterial plasmids. Plasmids containing the ribosomal DNA were identified by their ability to hybridize t
Publikováno v:
Biochemistry. 20:2639-2644
Chloroplast DNA from Euglena gracilis was used to construct a partial library of recombinant plasmids representing 45% of the DNA. Each plasmid was radioactively labeled in vitro by nick translation and hybridized in liquid to a vast excess of total
Publikováno v:
Biochimica et biophysica acta. 563(1)
The sequence organization of nuclear DNA in the single-celled alga Euglena gracilis has been studied by a combination of techniques: (1) the comparison of the reassociation kinetics of DNA fragments 300, 2000 and 8100 nucleotides long; (2) the reasso
Autor:
James R. Y. Rawson, Cindy L. Boerma
Publikováno v:
Biochemical and biophysical research communications. 89(2)
Transcription of Euglena chloroplast DNA was visualized by autoradiography of hybrids formed between electrophoretically separated EcoRI endonuclease fragments and [32P]-pulse labeled RNA prepared from cells at different stages of chloroplast develop
Autor:
James R. Y. Rawson, Cindy L. Boerma
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 73(7)
The number of chloroplast DNA molecules in Euglena gracilis cells was measured by determining the shift in the observed second-order rate constant for the reassociation of 125 I-labeled chloroplast DNA in the presence of unlabeled total cell DNA. Cel