Role for the IFT-A Complex in Selective Transport to the Primary Cilium

Autor: Wenxiang Fu, Lei Wang, Sehyun Kim, Ji Li, Brian David Dynlacht

Publikováno v: Cell Reports, Vol 17, Iss 6, Pp 1505-1517 (2016)

Intraflagellar transport sub-complex A (IFT-A) is known to regulate retrograde IFT in the cilium. To rigorously assess its other possible roles, we knocked out an IFT-A subunit, IFT121/WDR35, in mammalian cells and screened the localization of more t

Externí odkaz: https://doaj.org/article/cc98e842bd024865b222b723411c8268

Comparison of Ciliary Targeting of Two Rhodopsin-Like GPCRs: Role of C-Terminal Localization Sequences in Relation to Cilium Type

Autor: Abhishek Chadha, David S. Williams, Antonio E. Paniagua

Publikováno v: J Neurosci
The Journal of neuroscience : the official journal of the Society for Neuroscience, vol 41, iss 36

Primary cilia exhibit a distinct complement of proteins, including G-protein-coupled receptors (GPCRs) that mediate sensory and developmental signals. The localization of GPCRs to the ciliary membrane involves ciliary localization sequences (CLSs), b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c7bbba12950bde92e1a211f459828b1
https://doi.org/10.1523/jneurosci.0357-21.2021

STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena

Autor: Zhu Zhou, Jarema Malicki, Natalia A. Bulgakova, Dorota Wloga, Khodor S. Hazime, Ewa Joachimiak

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Hazime, K 2021, ' STORM imaging reveals the spatial arrangement of transition zone components and IFT particles at the ciliary base in Tetrahymena ', Scientific Reports, vol. 11, no. 1, 7899 . https://doi.org/10.1038/s41598-021-86909-5
Scientific Reports

The base of the cilium comprising the transition zone (TZ) and transition fibers (TF) acts as a selecting gate to regulate the intraflagellar transport (IFT)-dependent trafficking of proteins to and from cilia. Before entering the ciliary compartment

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5acfd215b41b18c3ff92811e9643cce
https://doaj.org/article/ad03647e47844e35a88866b74e826023

Formation of the B9-domain protein complex MKS1–B9D2–B9D1 is essential as a diffusion barrier for ciliary membrane proteins

Autor: Shuhei Chiba, Luxiaoxue Liang, Ryota Takei, Kazuhisa Nakayama, Yohei Katoh, Takuya Kobayashi, Misato Okazaki

Publikováno v: Molecular Biology of the Cell

Cilia are plasma membrane protrusions that act as cellular antennae and propellers in eukaryotes. To achieve their sensory and motile functions, cilia maintain protein and lipid compositions that are distinct from those of the cell body. The transiti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::deb1a6c56c2bb37eaf5949083c8f17e9
https://doi.org/10.1091/mbc.e20-03-0208

Analysis of ciliary status via G-protein-coupled receptors localized on primary cilia

Autor: Akie Hamamoto, Yumiko Saito, Yuki Kobayashi

Publikováno v: Microscopy. 69:277-285

G-protein-coupled receptors (GPCRs) comprise the largest and most diverse cell surface receptor family, with more than 800 known GPCRs identified in the human genome. Binding of an extracellular cue to a GPCR results in intracellular G protein activa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d81c3af582b2014d5498bd23ee97217e
https://doi.org/10.1093/jmicro/dfaa035