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pro vyhledávání: '"Ciarán, O'Fágáin"'
Publikováno v:
Protein Chromatography
Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical mat
Autor:
Ciarán, O'Fágáin
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 681
This article defines protein stability, emphasizes its importance and surveys some notable recent publications (2004-2008) in the field of protein stability/stabilization. Knowledge of the factors stabilizing proteins has emerged from denaturation st
Autor:
Ciarán, O'Fágáin
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 681
This article outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation, and the use
Autor:
Ciarán, O'Fágáin
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 244
Autor:
Ciarán, O'Fágáin
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 244
Publikováno v:
Biotechnology and bioengineering. 81(2)
Phthalic anhydride (PA) modification stabilizes horseradish peroxidase (HRP) by reversal of the positive charge on two of HRP's six lysine residues. Native and PA-HRP had half-inactivation temperatures of 51 and 65 degrees C and half-lives at 65 degr