TIM-3, LAG-3, or 2B4 gene disruptions increase the anti-tumor response of engineered T cells.

Autor: Cianciotti BC; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Magnani ZI; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Ugolini A; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Camisa B; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.; Innovative Immunotherapies Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Merelli I; Institute for Biomedical Technologies, National Research Council, Segrate, Italy., Vavassori V; Gene Transfer Technologies and New Gene Therapy Strategies Unit, San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), IRCCS San Raffaele Scientific Institute, Milan, Italy., Potenza A; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Imparato A; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Manfredi F; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Abbati D; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Perani L; Experimental Imaging Centre, IRCCS San Raffaele Scientific Institute, Milan, Italy., Spinelli A; Experimental Imaging Centre, IRCCS San Raffaele Scientific Institute, Milan, Italy., Shifrut E; The School of Neurobiology, Biochemistry and Biophysics, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.; Department of Pathology, Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.; Dotan Center for Advanced Therapies, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel., Ciceri F; Hematology and Bone Marrow Transplantation Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.; Università Vita-Salute San Raffaele, Milan, Italy., Vago L; Università Vita-Salute San Raffaele, Milan, Italy.; Unit of Immunogenetics, Leukemia Genomics and Immunobiology, IRCCS San Raffaele Scientific Institute, Milan, Italy., Di Micco R; San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Milan, Italy., Naldini L; Gene Transfer Technologies and New Gene Therapy Strategies Unit, San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), IRCCS San Raffaele Scientific Institute, Milan, Italy.; Università Vita-Salute San Raffaele, Milan, Italy., Genovese P; Gene Transfer Technologies and New Gene Therapy Strategies Unit, San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), IRCCS San Raffaele Scientific Institute, Milan, Italy.; Gene Therapy Program, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Department of Pediatric Oncology, Harvard Medical School, Boston, MA, United States., Ruggiero E; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy., Bonini C; Experimental Hematology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.; Università Vita-Salute San Raffaele, Milan, Italy.

Publikováno v: Frontiers in immunology [Front Immunol] 2024 Feb 29; Vol. 15, pp. 1315283. Date of Electronic Publication: 2024 Feb 29 (Print Publication: 2024).

Harnessing T cell exhaustion and trogocytosis to isolate patient-derived tumor-specific TCR.

Autor: Manfredi F; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Stasi L; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Buonanno S; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Marzuttini F; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Noviello M; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Mastaglio S; IRCCS San Raffaele Scientific Institute, Hematology and Hematopoietic Stem Cell Transplantation Unit, via Olgettina 60, Milan 20132, Italy., Abbati D; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Potenza A; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Balestrieri C; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy.; Center for Omics Sciences, IRCCS San Raffaele Scientific Institute, via Olgettina 60, Milan 20132, Italy., Cianciotti BC; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Tassi E; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Feola S; University of Helsinki, ImmunoVirotherapy Lab, Yliopistonkatu 4, 00100 Helsinki, Finland., Toffalori C; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Disease, Unit of Immunogenetics, Leukemia Genomics and Immunobiology, via Olgettina 60, Milan 20132, Italy., Punta M; Center for Omics Sciences, IRCCS San Raffaele Scientific Institute, via Olgettina 60, Milan 20132, Italy.; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Disease, Unit of Immunogenetics, Leukemia Genomics and Immunobiology, via Olgettina 60, Milan 20132, Italy., Magnani Z; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Camisa B; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Tiziano E; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy., Lupo-Stanghellini MT; IRCCS San Raffaele Scientific Institute, Hematology and Hematopoietic Stem Cell Transplantation Unit, via Olgettina 60, Milan 20132, Italy., Branca RM; Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institute, 171 65 Solna, Sweden., Lehtiö J; Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institute, 171 65 Solna, Sweden., Sikanen TM; Drug Research Program, Faculty of Pharmacy, Division of Pharmaceutical Chemistry and Technology, Helsinki University,, Viikinkaari 5E, 00014 Helsinki, Finland., Haapala MJ; Drug Research Program, Faculty of Pharmacy, Division of Pharmaceutical Chemistry and Technology, Helsinki University,, Viikinkaari 5E, 00014 Helsinki, Finland., Cerullo V; University of Helsinki, ImmunoVirotherapy Lab, Yliopistonkatu 4, 00100 Helsinki, Finland., Casucci M; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Disease, Innovative Immunotherapies Unit, via Olgettina 60, Milan 20132, Italy., Vago L; IRCCS San Raffaele Scientific Institute, Hematology and Hematopoietic Stem Cell Transplantation Unit, via Olgettina 60, Milan 20132, Italy.; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Disease, Unit of Immunogenetics, Leukemia Genomics and Immunobiology, via Olgettina 60, Milan 20132, Italy.; Vita Salute San Raffaele University, Milan, Italy., Ciceri F; IRCCS San Raffaele Scientific Institute, Hematology and Hematopoietic Stem Cell Transplantation Unit, via Olgettina 60, Milan 20132, Italy.; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Disease, Innovative Immunotherapies Unit, via Olgettina 60, Milan 20132, Italy., Bonini C; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy.; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation and Infectious Disease, Innovative Immunotherapies Unit, via Olgettina 60, Milan 20132, Italy., Ruggiero E; IRCCS San Raffaele Scientific Institute, Division of Immunology, Transplantation, and Infectious Diseases, Experimental Hematology Unit, via Olgettina 60, Milan 20132, Italy.

Publikováno v: Science advances [Sci Adv] 2023 Dec; Vol. 9 (48), pp. eadg8014. Date of Electronic Publication: 2023 Dec 01.

CRISPR-based gene disruption and integration of high-avidity, WT1-specific T cell receptors improve antitumor T cell function.

Autor: Ruggiero E; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Carnevale E; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Prodeus A; Intellia Therapeutics, Cambridge, MA 02139, USA., Magnani ZI; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Camisa B; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Merelli I; San Raffaele Telethon Institute for Gene Therapy (SR-TIGET), Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy.; National Research Council, Institute for Biomedical Technologies, Segrate, Italy., Politano C; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Stasi L; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Potenza A; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy.; School of Medicine and Surgery, Milano-Bicocca University, 20126 Milan, Italy., Cianciotti BC; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Manfredi F; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy.; Vita-Salute San Raffaele University, 20132 Milan, Italy., Di Bono M; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Vago L; Immunogenetics, Leukemia Genomics and Immunobiology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy.; Hematology and Bone Marrow Transplantation Unit, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Tassara M; Immunohematology and Transfusion Medicine Unit, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Mastaglio S; Hematology and Bone Marrow Transplantation Unit, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Ponzoni M; Vita-Salute San Raffaele University, 20132 Milan, Italy.; Pathology Unit, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Sanvito F; Pathology Unit, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Liu D; Intellia Therapeutics, Cambridge, MA 02139, USA., Balwani I; Intellia Therapeutics, Cambridge, MA 02139, USA., Galli R; Neural Stem Cell Biology Unit, Division of Neurosciences, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Genua M; Genomics of the Innate Immune System Unit, San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Ostuni R; Vita-Salute San Raffaele University, 20132 Milan, Italy.; Genomics of the Innate Immune System Unit, San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Doglio M; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., O'Connell D; Intellia Therapeutics, Cambridge, MA 02139, USA., Dutta I; Intellia Therapeutics, Cambridge, MA 02139, USA., Yazinski SA; Intellia Therapeutics, Cambridge, MA 02139, USA., McKee M; Intellia Therapeutics, Cambridge, MA 02139, USA., Arredouani MS; Intellia Therapeutics, Cambridge, MA 02139, USA., Schultes B; Intellia Therapeutics, Cambridge, MA 02139, USA., Ciceri F; Vita-Salute San Raffaele University, 20132 Milan, Italy.; Hematology and Bone Marrow Transplantation Unit, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy., Bonini C; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, Ospedale San Raffaele Scientific Institute, 20132 Milan, Italy.; Vita-Salute San Raffaele University, 20132 Milan, Italy.

Publikováno v: Science translational medicine [Sci Transl Med] 2022 Feb 09; Vol. 14 (631), pp. eabg8027. Date of Electronic Publication: 2022 Feb 09.

TCR Redirected T Cells for Cancer Treatment: Achievements, Hurdles, and Goals.

Autor: Manfredi F; Vita-Salute San Raffaele University, Milan, Italy.; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Cianciotti BC; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.; Fondazione Centro San Raffaele, Milan, Italy., Potenza A; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.; School of Medicine and Surgery, University of Milano - Bicocca, Milan, Italy., Tassi E; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Noviello M; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Biondi A; Clinica Pediatrica Università degli Studi di Milano Bicocca, Fondazione MBBM, Monza, Italy., Ciceri F; Vita-Salute San Raffaele University, Milan, Italy.; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Bonini C; Vita-Salute San Raffaele University, Milan, Italy.; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Ruggiero E; Experimental Hematology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Publikováno v: Frontiers in immunology [Front Immunol] 2020 Sep 03; Vol. 11, pp. 1689. Date of Electronic Publication: 2020 Sep 03 (Print Publication: 2020).

Tailored chemokine receptor modification improves homing of adoptive therapy T cells in a spontaneous tumor model.

Autor: Garetto S; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Sardi C; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Martini E; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Roselli G; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Morone D; Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Angioni R; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Cianciotti BC; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Trovato AE; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Franchina DG; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Castino GF; Laboratory of Cellular Immunology, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Vignali D; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Erreni M; Laboratory of Cellular Immunology, Humanitas Clinical and Research Center, Rozzano (Milano), Italy., Marchesi F; Laboratory of Cellular Immunology, Humanitas Clinical and Research Center, Rozzano (Milano), Italy.; Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano, Milan, Italy., Rumio C; Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy., Kallikourdis M; Adaptive Immunity Laboratory, Humanitas Clinical and Research Center, Rozzano (Milano), Italy.; Humanitas University, Rozzano (Milano), Italy.

Publikováno v: Oncotarget [Oncotarget] 2016 Jul 12; Vol. 7 (28), pp. 43010-43026.